Reagent for testing foot and mouth disese virus by fluorescent quantity RI-PCR and its preparation process

Abstract

The invention relates to a biopreparate designed and synthesized by applying primer Express and primer prere 5.0 softwares and using the gene fragment of foot and mouth disease virus as a target, especially a reagent able to detect the foot and mouth disease virus and a method of preparing the reagent. The fluorescent quantitative RT-PCR detecting reagent of foot and mouth disease virus contains a pair of specific primers and a specific fluorescent probe. It uses the most conservative fragment of the gene sequence in FMDV 3D region for the first time to design and synthesize the primers and probe, and builds the fluorescent quantitative RT-PCR and prepares a detecting reagent box, and can rapidly detect FMDV in cell culture substance, hydatid fluid, hydatid skin and secretion, blood, and meat products. It creates a fluorescent quantitative RT-PCR of FMDV.

Description

technical field [0001] The invention relates to a biological agent designed and synthesized by using primer Express software and primer prere5.0 software, especially a reagent capable of detecting the foot-and-mouth disease virus and a preparation method of the reagent, taking the foot-and-mouth disease virus gene fragment as the target. Background technique [0002] Foot-and-mouth disease (FMD) is a highly contagious disease of wild and domestic artiodactyls caused by foot-and-mouth disease virus (FMDV). Rapid and reliable diagnosis is crucial to the control of FMD. In order to take effective measures, laboratory diagnosis must make rapid and accurate conclusions within 24 hours. Foot-and-mouth disease caused by viruses in animal and meat products has been the main cause of several pandemics. Methods for accurate detection of low levels of FMD virus in meat products, recessive infection or persistently infected hosts must have high sensitivity, high specificity and high ac...

AI Technical Summary

Problems solved by technology

Conventional diagnostic methods, such as virus isolation, serological tests, animal tests, etc., are time-consuming and labor-intensive, or have low specificity and sensitivity, and cannot meet the requirements of rapid and accurate diagnosis

RT-PCR, competitive PCR and ELISA-PCR methods overcome the shortcomings of these methods and can be used for the diagnosis of FMD, but they also have disadvantages such as time-consuming, easy to pollute, electrophoresis detection after amplification, and a small number of samples for each detection.