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A kind of erythrocyte sedimentation rate standard substance and preparation method thereof

A technology for erythrocyte sedimentation and standard product, applied in the medical field, can solve the problem of high preparation cost, and achieve the effects of economical source, no biological safety hazards, and controllable stability.

Active Publication Date: 2018-04-17
珠海朗泰生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, only some advanced countries in the world have such products. The preparation cost is high and the standard product has only one value.

Method used

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  • A kind of erythrocyte sedimentation rate standard substance and preparation method thereof

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Embodiment 1

[0016] see figure 1 As shown, the present invention provides a kind of erythrocyte sedimentation rate standard product and preparation method thereof, first utilize glucose 15g / L, boric acid 6g / L, sodium borate 2g / L, adenine 3g / L, sodium fluoride 1g / L to be configured into Cell activity protection solution (liquid A), then utilize disodium hydrogen phosphate 36g / L, sodium bicarbonate 31g / L, glutaraldehyde 25% 25mg / L, propylene glycol 3g / L, calf serum 0.9g / L to be configured into Cell activity preservation solution (liquid B); then take the fresh blood of slaughtered livestock that has passed the inspection and quarantine and anticoagulate with 6:1 EDTA, remove the supernatant after centrifugation for 5 minutes, wash the bottom cells with a protective agent, centrifuge, Repeat three times, keep the lower layer of red blood cells, add 20 times the preservative and mix well for 1 hour, remove the supernatant after standing for 24 hours, collect the prepared mixture and mix it in ...

Embodiment 2

[0019] First, use glucose 25g / L, boric acid 5g / L, sodium borate 1g / L, adenine 5g / L, and sodium fluoride 2g / L to prepare a cell activity protection solution (A solution), then use disodium hydrogen phosphate 36g / L, Sodium bicarbonate 31g / L, glutaraldehyde 25% 25mg / L, propylene glycol 3g / L, and calf serum 0.9g / L were prepared into a cell viability preservation solution (B liquid); Anticoagulate the fresh blood of domestic animals with 6:1 EDTA, centrifuge for 5 minutes, remove the supernatant, wash the bottom cells with a protective agent, and centrifuge for three times, keep the lower red blood cells, add 20 times the preservative and mix well for 1 hour. After standing for 24 hours, remove the supernatant, collect the prepared mixture and mix it in the protective agent (solution A) at a ratio of 1:1 to make humanoid red blood cells; Red blood cell granules are injected into the scale of Wei's ESR tube according to the following concentration ratio, and stand at room temperatur...

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Abstract

A kind of erythrocyte sedimentation rate standard substance and preparation method thereof, including imitation human erythrocytes, protective agent (A liquid) and preservative (B liquid) placed in Wei's erythrocyte sedimentation tube, the preparation method is as follows; Step1, take the tested The fresh blood of livestock qualified for quarantine is anticoagulated with 6:1 EDTA, and the supernatant is removed after centrifugation for 5 minutes; Step2, the bottom cells after centrifugation are washed with solution A, and then centrifuged again, and the bottom red blood cells are retained; Step3, the The reserved red blood cells in the lower layer were added with 20 times of B solution and mixed thoroughly for 1 hour, and the supernatant was removed after standing for 24 hours; Step4, and then the collected and prepared mixture was mixed in A solution at a ratio of 1:1 to make the imitation Human red blood cell granules, through the flow cytometer or hematology analyzer, calculate the exact number of cells; Step5, inject the human red blood cell granules and sodium citrate in different proportions and concentrations in a 1:1 mixture At the scale inside the Weisser's ESR tube, let it stand at room temperature for 1 hour, and then seal it for storage.

Description

technical field [0001] The invention relates to the medical field, in particular to an erythrocyte sedimentation rate standard product and a preparation method thereof. Background technique [0002] Erythrocyte sedimentation rate, referred to as erythrocyte sedimentation rate, refers to the natural sinking rate of red blood cells in anticoagulated whole blood in vitro under specified conditions. The erythrocyte sedimentation standard is the metering substance for the determination of the erythrocyte sedimentation analyzer, operating technology, and quantitative analysis of the traceability of the environment. It is the only basis for the evaluation of the quality control of the room and the outside of the erythrocyte sedimentation rate analysis and to ensure the accuracy and precision of clinical test results. , but also according to user needs for high, medium and low value. At present, only some advanced countries in the world have such products. The preparation cost is h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/05G01N1/28
Inventor 丁焕红王骕朱青王文东
Owner 珠海朗泰生物科技有限公司
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