Detection method for newcastle disease virus

Abstract

The invention provides a detection method for a newcastle disease virus. The method comprises operating steps of performing homology comparison by selecting a nucleoprotein (NP) gene sequence of the newcastle disease virus in an available DNA sequence database (GENEBANK), and designing primers and probes corresponding to each gene segment according to a conserved domain; synthesizing and modifying the primers and the probes, coupling the specificity probes with fluorescent-encoded microspheres to prepare specificity detection microspheres (namely a liquid chip). The liquid chip can perform specific recognition of a PCR amplification product of each of the gene segments and detection results can be read from a liquid chip detector. The detection method has advantages of high sensitivity, strong specificity, high repeatability, simple operation, short consumed time, relatively low cost, using safety, and the like.

Description

technical field [0001] The invention relates to the technical field of poultry infection virus detection, in particular to a detection method for Newcastle disease virus. Background technique [0002] Newcastle disease (ND) is an acute, febrile, septic and highly contagious infectious disease of poultry caused by Newcastle disease virus (NDV). It is characterized by high fever, dyspnea, diarrhea, nervous disturbance, and mucosal and serosal hemorrhage. It has a high morbidity and fatality rate and is a major infectious disease that endangers the poultry industry. The World Organization for Animal Health (OIE) classifies it as a category A disease. The current surveillance methods for Newcastle disease virus include traditional methods and molecular biology methods. Traditional methods, such as virus isolation and culture and serological diagnosis, have the disadvantages of cumbersome operation and long diagnosis time, while molecular biological methods such as RT-PCR are ...

AI Technical Summary

Problems solved by technology

Traditional methods, such as virus isolation and culture and serological diagnosis, have the disadvantages of cumbersome operation and long diagnosis time, while molecular biological methods such as RT-PCR are specific, sensitive, convenient and quick, and overcome the limitations of traditional methods. Limitations, but high-throughput testing cannot be performed, so it cannot meet the customs' entry-exit quarantine requirements

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