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An identification method, blood technology, applied in the field of blood identification, can solve the problems of slow measurement speed, hidden dangers of biological safety, human eye damage, etc.
Active Publication Date: 2016-10-26
盈开生物科技(上海)有限公司
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Problems solved by technology
[0005] The existing methods have the following problems: 1. Due to the need for mechanical movement, the measurement speed is relatively slow
2. The laser will cause damage to the human eye, and there are hidden dangers to biological safety
3. When there is a problem with the device, the operator cannot detect it
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Embodiment 1
[0036] see figure 2 , the present invention discloses a blood stratification identification method, the identification method includes the following steps:
[0037] [Step S1 ] Calibrate the sample tube.
[0038] Add liquid with a volume of V1 to the sample tube, measure its first physical height Hphysic1, take a picture of the sample tube at a fixed object distance, set the region of interest ROI on the photo, and record its corresponding position in the image HpictureCalib1 ;
[0039] Make a calibration every set unit volume, record its physical height Hphysic, and the corresponding position HpictureCalib in the image, and form a calibration table;
[0040] [Step S2] Identify the boundaries of the blood in the sample tube.
[0041] Take a picture of the sample tube at a fixed object distance, and there are 2 color change positions in the ROI of the image region of interest, such as image 3 shown. see Figure 4 , count the sum of the gray values of all pixels at each...
Embodiment 2
[0048] A blood stratification identification method, said identification method comprising the steps of:
[0049] Step S1, calibrate the sample tube; perform a calibration every set unit volume, and record its physical height and corresponding position in the image;
[0050] Step S2, identify the boundary of the blood in the sample tube; take a picture of the sample tube at a fixed object distance, and there are 2 color change positions in the ROI of the image; count the gray value of all pixels at each height of the ROI in the ROI The sum of the generated pixel curves has two cliff-like drops. In the pixel curve diagram, the corresponding sample tube image finds the first jump position from top to bottom. In the pixel curve diagram, the corresponding sample tube image goes from bottom to bottom. Find the position of the first upward jump, so as to obtain the specific image positions at the two boundaries; the difference between the specific image positions at the two boundari...
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Abstract
The invention discloses a blood layered identification method, which comprises the steps of: calibrating a sample tube; making a calibration every other set unit volume, recording physical heights of the calibrations and corresponding positions in an image; identifying boundary of blood in the sample tube; and executing a volume calculation step including the processes of calculating volume by adopting a piecewise interpolation algorithm, conducting interpolating calculation when calculating a result according to intervals to which pixel positions belong, acquiring the volume V1 and the pixel position P1 of a first calibration point as well as the volume V2 and the pixel position P2 of a second calibration point from the calibration data, and calculating V3 according to similar triangles when the pixel position of a point to be calculated is P3. The blood layered identification method can utilize 2 or even 4 cameras for shooting 16 samples at one time, and the speed is dozens of times faster than that of laser scanning. By adopting the blood layered identification method, biological safety hazards do not exist, each sample has a picture, and the operator can find out interference easily when encountering the interference.
Description
technical field [0001] The invention belongs to the technical field of blood identification, and relates to a blood identification method, in particular to a blood layer identification method. Background technique [0002] Blood is composed of plasma white blood cells and blood cells. Due to the different density of the three, after centrifugation, it will be divided into 3 layers. See figure 1 shown. [0003] Human DNA only exists in plasma, so all DNA sequencing only needs to extract plasma. In automated testing, it is necessary to automatically determine the volume of plasma. Since the diameter of the tube is known, the problem can be converted to air and plasma. The height H1 of the stratification, and the height H2 of the plasma and white blood cells are judged. [0004] Existing blood layer identification usually adopts laser scanning method. The sample tube is grasped to the measurement position by an automatic manipulator, scanned from top to bottom, and H1 and H2 ...
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