Multi-fluorescent staining solution for genital tract secretions as well as preparation method and application of multi-fluorescent staining solution

Abstract

The invention belongs to the technical field of microbial fluorescent staining, and particularly relates to a genital tract secretion multi-fluorescent staining solution as well as a preparation method and application thereof. The genital tract secretion multi-fluorescent staining solution comprises fluorescein, nucleic acid dye, a bacteriostatic agent, an anti-quenching agent, a permeable agent and a buffer solution, and the pH is 4.5 to 6.0. The genital tract secretion multiple-fluorescence staining solution has short staining time on vaginal secretion samples, and is suitable for large-scale clinical work; the staining result is stable and reliable, the morphological structures of cells and various pathogenic microorganisms are clear, the contrast is distinct, and the morphology, size and distribution of various pathogenic microorganisms and the variety and number of various cells in the smear can be clearly observed under a microscope. In addition, the multi-fluorescent staining solution is good in stability and can be used for a long time, and the staining cost is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of microbial fluorescent dyeing, in particular to a multiple fluorescent dyeing solution for reproductive tract secretions and its preparation method and application. Background technique [0002] The World Health Organization (WHO) shows to global women's health investigation and research, the global women's vaginitis incidence rate is 13.2%, and the recurrence rate is more than 80%. Vaginal inflammation caused by female reproductive tract infection mainly includes bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), trichomonas vaginitis (TV), aerobic vaginitis (AV), cell Lytic vaginosis (CV) and mixed infections. [0003] Vaginal secretion examination is the most common examination item in gynecology, and it is the simplest and most commonly used examination method for diagnosing female external genital tract infection. Through the inspection of the appearance, cleanliness, epithelial cells, white ...

AI Technical Summary

Problems solved by technology

This kind of culture method has a long cycle, strict requirements on equipment and environment, requires a complete set of microbial culture medium identification equipment, and has high requirements on operators, which limits its clinical application

[0007] In recent years, there are some fluorescent staining solutions and corresponding detection kits (fluorescence method) on the market, but these methods have various disadvantages: (1) The detection target is single, and each reagent can only detect one microorganism; (2) Two reagents are required for two staining, and the operation steps are cumbersome; (3) It needs to be incubated for 30 minutes, and the operation time is long; (4) Different microscope light sources need to be switched during the observation process

In short, the operation is very cumbersome and time-consuming, which is not conducive to carrying out a large number of clinical diagnostic work

For example, patent CN110940646A discloses a double fluorescent staining solution for vaginal microorganisms and its application. The disclosed fluorescent staining solution has two reagents, staining solution A and staining solution B, and requires two steps of dyeing, although it can effectively detect pathogenic microorganisms. , but the operation is cumbersome

Another example is that patent CN111019999A discloses a microbial fluorescent staining solution and its application. The disclosed microbial fluorescent staining solution includes lectin, fluorescein, nucleic acid dye, buffer solution, anti-fade agent, bacteriostatic agent and water. The buffer solution is preferably a phosphate buffer solution. This patent has the disadvantages that the phosphate buffer cannot be stored for a long time, is prone to precipitation, and affects the dyeing effect.

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