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Acid-fast staining method and quality monitoring method

A technology for acid-resistant dyeing and quality monitoring, which is applied in the direction of measuring devices, instruments, and analytical materials, and can solve problems such as inability to decolorize

Active Publication Date: 2021-09-10
GUANGZHOU KINGMED DIAGNOSTICS CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to address the above problems and provide a quality monitoring method for acid-fast dyeing, which can monitor the quality of the acid-resistant dyeing process and avoid the problem of inability to decolorize

Method used

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  • Acid-fast staining method and quality monitoring method
  • Acid-fast staining method and quality monitoring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Exploration of the causes of inability to decolorize in acid-fast staining.

[0038] 1. Find the problem.

[0039] The inventor carried out acid-fast staining according to the conventional method, and found that the background of acid-fast staining sometimes appeared to be red-stained, which could not be removed by 20% sulfuric acid decolorization. figure 1 As shown, the tissue section is generally purple-red, the background is not well differentiated, and the contrast is poor, making it difficult to distinguish whether there are mycobacteria.

[0040] After re-slicing and staining, the section was slightly reddish under the microscope, as shown in the microscope. figure 2 As shown, Mycobacterium tuberculosis is red in number and easy to observe. The background is light blue and the contrast is clear.

[0041] 2. Preliminary screening of the reasons.

[0042] In order to find the root of the problem systematically, we list the key links and elements in the experimen...

Embodiment 2

[0060] Study on the standard of formaldehyde content in the quality control of acid-fast dyeing.

[0061] 1. Method.

[0062] During the experiment, the environmental formaldehyde concentration monitoring link was set up to compare the effects of different environmental formaldehyde concentrations on acid staining.

[0063] 2. Results.

[0064] After sorting out the experimental results, it was found that when the concentration of formaldehyde in the environment is less than 0.20mg / m 3 When the section is stained, it can be successfully decolorized and differentiated with an acid decolorizer. After the section is washed with water, the tissue is slightly light red, and the background is clean under the microscope, and Mycobacterium tuberculosis can be clearly observed; when the concentration of formaldehyde in the environment is greater than 0.20mg / m 3 The red background of the section cannot be decolorized with acid decolorizer. After the section is washed with water, the...

Embodiment 3

[0067] A method for monitoring the quality of acid-fast staining, including a formaldehyde detection step in the staining incubation process, specifically as follows:

[0068] 1. Method: Put the formaldehyde detection reagent in the corner of the incubation box (transparent, with a cover, convenient for observing the situation in the box), place the absorption box in the formaldehyde detection kit (brand: Lvzhiyuan, model: Z-0434), Then pour all the detection solution in the formaldehyde detection kit into the absorption box and mix well. Cover the lid of the incubation box and wait for the reagents to react for 30 minutes. Pour the chromogen in the formaldehyde detection kit into the absorption box, mix well, and let stand for 10 minutes.

[0069] 2. Observation results: Use the absorption box and the color comparison card in the formaldehyde detection kit to perform color comparison, and obtain the result of the formaldehyde concentration in the incubation box.

[0070] 3....

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Abstract

The invention relates to an acid-fast staining method and a quality monitoring method, and belongs to the technical field of tissue staining. The method comprises a formaldehyde detection step in the staining incubation process, when the formaldehyde concentration in the incubation environment is detected to be smaller than a threshold value, the staining process is judged to be qualified, and otherwise, the staining process is judged to be unqualified. By adopting the method, the quality of the acid-resistant staining process can be monitored, the problem of incapability of decoloring is avoided, and the detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of tissue staining, in particular to an acid-fast staining method and a quality monitoring method. Background technique [0002] Acid-fast staining is a common method for displaying mycobacteria such as Mycobacterium tuberculosis and Bacillus leprae. The cell wall of mycobacteria contains a large amount of lipids, which can resist the decolorization of acid decolorizing agents (hydrochloric acid, sulfuric acid, etc.) after being combined with phenol basic fuchsin dye, so it is also called acid-fast bacillus. [0003] In acid-fast staining, the step of decolorization and differentiation with acid decolorizer is very important. If the decolorization is not clean, the background of the whole tissue section will be red, which will greatly interfere with the observation of mycobacteria under the microscope. Sometimes in the laboratory process, the background of acid-fast staining is red, and it cannot be removed...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/34G01N1/28G01N21/78
CPCG01N1/30G01N1/34G01N1/286G01N21/78G01N21/783G01N2001/2873
Inventor 钟学军黎锋孙成刚赖辉龙张杰覃韦双
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT
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