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Routine paraffin wax flaking HE dyeing non-xylol whole-course treatment liquid for biological organization sample

A biological tissue and xylene technology, applied in the field of in vitro diagnostic reagents, can solve problems such as waste of resources, complicated operation, lack of uniform standards, etc., and achieve the effect of stable use, simple and fast operation, and easy mastery

Inactive Publication Date: 2009-01-14
赵晓辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process puts operators in a toxic environment, complicates operations, wastes resources, and lacks uniform standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The tissue fixative set number of the conventional paraffin-waxed HE staining and xylene-free full treatment solution of the biological tissue sample of the present invention is the first tank, the tissue dehydrating solution I is the second tank, and the tissue dehydrating solution II is the third tank. The transparent liquid is the fourth tank, the tissue wax solution I is the fifth tank, and the tissue wax solution II is the sixth tank. When biological tissue samples are dehydrated, the biological tissue samples are put into the first to sixth tanks in sequence. The operation requirements are as follows:

[0042] Putting into the first tank: 16:00 to 19:30 that day; (Soaking time: 3 hours and 30 minutes)

[0043] Time to put in the second tank; 19:30 to 21:00 that night; (Soaking time: 1 hour 30 minutes)

[0044] Time to put in the third tank: 21:30 to 22:30 that day; (Soaking time: 1 hour 30 minutes)

[0045] Putting into the fourth tank: 22:30 in the evening of the sam...

Embodiment 2

[0050]The section dewaxing solution I described in the general treatment solution for HE staining of conventional paraffin slices of biological tissue samples of the present invention without xylene is the seventh tank, the section dewaxing solution II is the eighth tank, and the hematoxylin staining solution is the ninth tank. , Eosin staining solution is the tenth tank, the de-floating solution is the eleventh tank, the dewatering clear solution is the twelfth tank, and the slice clear solution is the thirteenth tank.

[0051] The following is the staining process of biological tissue sample sections:

[0052] (1) Put the biological tissue sample slice into the seventh tank and soak for five minutes, then take it out and put it in the eighth tank.

[0053] (2) Immerse the biological tissue sample slice in the eighth tank for five minutes and take it out;

[0054] (3) Wash the biological tissue sample section with tap water for two minutes, then put it in the ninth tank, soak in ...

Embodiment 3

[0059] Procedure requirements for HE staining of large and small tissue sections:

[0060] (1) The HE staining method and time of large tissue and micro tissue sections are the same. The frosted part of the slide can only be marked with a 2B pencil, otherwise the writing will be difficult to retain. When the room temperature is above 15°C, the wax block should be cooled on ice to prevent the wrinkles from opening and sectioning. The thickness of the tissue section is 3 to 4 μm, and the wrinkles are fully opened.

[0061] (2) How to use the anti-slipping solution: mainly used for blood clots and tissue slices that are easy to peel off. Each slice should be placed on the appropriate position of the slide with very little solution, and another slide can be attached and opened. Can be used, or wipe off excess anti-dropping tablets with your fingers.

[0062] (3) Drying treatment: The prepared tissue sections are dried in a wax melting incubator or in a dryer for 20 to 30 minutes.

[0...

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Abstract

The invention aims at providing a pathological diagnosis reagent, which is mainly used for processing various tissue samples in the pathology department in all levels of hospitals, can satisfy the requirements of a global non-xylene HE dyeing treating fluid of routine paraffin slices of biological tissue samples that is required in the slicing technique of the tissue samples with manual operation or operation of an automatic dehydrator and in the HE dyeing technique of cast-off cells, and is separated into two parts of tissue dehydration and section staining. As a novel conservation-conscious environmental protection technique, the pathological diagnosis reagent quickens splicing speed and simultaneously leads the tissue not to become over shrunk, hardened, split and crisp and leads the cells and the tissue structure to be closer to original patterns; the pathological diagnosis reagent has more complete tissue dehydration and adopts various novel mixed reagents that are pure analyzing organic chemical reagents with low volatility or non-volatility, non-toxicity and non-pollution, thus replacing originally widely used xylene reagents that have toxicity and heavy environmental pollution and eliminating occupational diseases that are caused by the xylene reagents in the global slicing of tissue sample preparation and tissue slice HE dyeing.

Description

(1) Technical field [0001] The invention relates to an in vitro diagnostic reagent in a medical device, in particular to a full-process treatment solution for HE staining without xylene in conventional paraffin slices of biological tissue samples. (2) Background technology [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, as long as the operation is performed, the pathology department of the hospital will make histopathological diagnosis on the surgical tissue samples. Conventional paraffin-embedded HE staining is still the most basic technique currently used to process tissue samples. It is the basis of pathological diagnosis methodology and a reference standard for evaluating the pros and cons of many new methods and technologies. Professor Chen Jie, a well-known pathologist from Peking Union Medical College Hospital in my country, emphasized at the conference many times in recent years: "Currently, pathological techniques are mostly a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 赵晓辉
Owner 赵晓辉
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