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Application of functional molecular marker of tomato fruit development associated genes

A technology of molecular markers and molecular markers, applied in the field of molecular biology, can solve problems such as inability to regulate tomato fruit development and ripening, and achieve efficient identification, simple operation, and reliable experimental results

Active Publication Date: 2020-05-22
SHENZHEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a functional molecular marker application of tomato fruit development-related genes, aiming to solve the problem that the development of tomato fruit cannot be accurately assessed in the prior art. and mature regulation issues

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  • Application of functional molecular marker of tomato fruit development associated genes
  • Application of functional molecular marker of tomato fruit development associated genes
  • Application of functional molecular marker of tomato fruit development associated genes

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Embodiment Construction

[0016] In order to make the purpose, technical solutions and technical effects of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than Full examples. In combination with the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0017] In the description of the present invention, it should be understood that the terms "first" and "second" are used for description purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly indicating the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these featu...

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Abstract

According to the invention, a functional molecular marker Sly_miRNA164b of tomato fruit development associated genes is used to express tomato fruit development associated genes, and then GUS stainingtechnology can be used to rapidly verify development status of plant fruits. The method has simple and easy operations and obtains reliable results. Meanwhile, the correlation of the Sly_miRNA164b toshape and maturity of tomato fruits is obtained through functional molecular marker analysis. The functional molecular marker can be used to quickly, accurately and efficiently identify the shape andmaturity of the tomato fruits, provides a basis for the screening of tomatoes and offers important way for molecular markers in identification of germplasm resources.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to the application of a functional molecular marker of a gene related to tomato fruit development. Background technique [0002] Tomato (Solanum lycopersicum), belonging to the family Solanaceae, is a berry rich in carbohydrates, organic acids, vitamins and minerals, and is also an important source of food and nutrition for humans. [0003] Tomato is a temperature-loving vegetable. Under normal conditions, the optimum temperature for assimilation is 20-25°C, and the optimum soil temperature for root growth is 20-22°C. It likes light, the light saturation point is 70000lx, and the suitable light intensity is 30000-50000lx. It is also a short-day plant, and tomato likes water. Generally, the soil humidity is 60-80%, and the air humidity is 45-50%. [0004] Tomato fruits can be divided into four stages during the ripening process, namely, the ripening stage (MG), the breaking stage (...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158C12Q2600/178
Inventor 张永夏朱小恩黄腾波
Owner SHENZHEN UNIV
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