Dynamic cell microenvironment simulation platform and preparation method thereof

A cell microenvironment and simulation platform technology, which is applied in the carrier field of simulating dynamic cell microenvironment, can solve the problem that the time and degree of cells and HAVDI cannot be precisely regulated, and achieve effective simulation of dynamic cell microenvironment, good biocompatibility, easy-to-operate effect

Active Publication Date: 2021-11-09
XI AN JIAOTONG UNIV
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However, the timing and extent of the interaction b

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  • Dynamic cell microenvironment simulation platform and preparation method thereof
  • Dynamic cell microenvironment simulation platform and preparation method thereof
  • Dynamic cell microenvironment simulation platform and preparation method thereof

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[0041] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. The embodiments are only used to explain the purpose, technical solutions and advantages of the present invention, but not to limit the protection scope of the present invention.

[0042] (1) Acquisition of a platform for simulating a dynamic cellular microenvironment

[0043] 1. Regulating the modification and removal of a ligand on a substrate

[0044] see figure 1 , modify multiple single-stranded DNA molecules on the substrate, and each single-stranded DNA molecule can modify the same ligand to the substrate by hybridizing with the corresponding ligand-DNA. This kind of ligand can be removed from the substrate through the corresponding replacement chain, so as to achieve the purpose of dynamic regulation of the ligand, as follows:

[0045] (1) Preparation of DNA1-modified PEG hydrogel: 5% w / v (g / mL) of 8-arm PEG-maleimide and 3% w / v (g / mL...

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Abstract

The invention discloses a dynamic cell microenvironment simulation platform and a preparation method thereof. The platform takes hydrogel as a cell culture carrier, and can selectively regulate and control the combination and dissociation of one or more ligands in the hydrogel and a cross-linked network structure of the hydrogel in real time, wherein the ligands are proteins and polypeptides with specific structures determined according to the types of cells and receptor types expressed by the cells, and are modified in the hydrogel based on a DNA hybridization principle, so that a dynamic cell microenvironment is simulated in vitro, and the platform can be used for regulating and controlling the interaction between the cells and the microenvironment. The platform is simple in preparation method, easy to operate and good in biocompatibility, and meanwhile, the physical and chemical properties in a biological environment are stable.

Description

technical field [0001] The invention belongs to the intersection field of biomaterials and biomechanics, and relates to a carrier that modifies various ligands through the principle of DNA hybridization, regulates the removal of various ligands in real time through the principle of DNA chain replacement reaction, and simulates a dynamic cell microenvironment. Background technique [0002] Cells are in a complex and dynamically changing microenvironment in vivo. The state of interaction between cells and the microenvironment is not static, but dynamic and even reversible. Many physiological or pathological processes involve dynamic interactions between cells and their microenvironment. Simulating the response of cells to the dynamic microenvironment in vitro can provide more experimental basis for obtaining ways to treat cancer and other diseases, and can provide effective and convenient experimental methods for regenerative medicine and tissue engineering. [0003] At pres...

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Application Information

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IPC IPC(8): C12N5/00C12N5/0775
CPCC12N5/0068C12N5/0662C12N2533/12C12N2533/50C12N2533/30C12N2537/00Y02A50/30
Inventor 林敏张政赵灵珠冯锦腾张欢
Owner XI AN JIAOTONG UNIV
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