A method for detecting the influence of cigarette smoke on cellular water permeability

Abstract

The invention relates to a detection method for influence of cigarette smoke on cell water permeability, which belongs to the technical field of biological application. A biochemical and cytology method is adopted, BMP6 is used for processing cells, a cell micro environment when a sjogren syndrome occurs is simulated, so that the influence of the cigarette smoke on the water permeability is researched, and compared with the animal experiment, the detection method is simpler and more efficient; an oral bionic simulation apparatus is adopted for trapping the cigarette smoke, thereby being closerto the actual human smoking state; and meanwhile, by adopting a high content system, the influence of a plurality of samples on the cell water permeability can be simultaneously detected at differenttime, the multi-sample detection can be rapidly, accurately and visibly performed, and a novel rapid means can be provided for the efficacy evaluation of the cigarette smoke.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a method for detecting the influence of cigarette smoke on the water permeability of cells. Background technique [0002] Saliva is the main factor to maintain the balance of the oral micro-ecological environment. It has many functions such as moistening the mouth and food, facilitating speaking and swallowing, removing food particles on the taste buds, so as to continuously taste the taste of food, cleaning and protecting the oral cavity. The sensory evaluation of cigarettes shows that some cigarette smoke can cause negative feelings such as dry mouth, dryness, and discomfort. Therefore, the development of cigarette products with the function of reducing dryness and promoting body fluids is of great significance for improving oral comfort and smoking quality of cigarettes. [0003] The aquaporin family (Aquaporins, AQP) is a group of proteins that spe...

AI Technical Summary

Problems solved by technology

Studies have shown that there is down-regulation or abnormal subcellular distribution of AQP5 in the salivary gland cells of Sjogren's syndrome patients and Sjögren's syndrome model mice, which leads to the imbalance of cellular water permeability

At present, existing studies have found that the hypotonic environment is easy to cause some changes in cell morphology, which makes volume measurement difficult under traditional fluorescence microscopes or confocal microscopes, cumbersome operations, and is not conducive to the detection of large quantities of samples.

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