A method for detecting the influence of cigarette smoke on cellular water permeability

A water permeability, cigarette smoke technology, applied in permeability/surface area analysis, measurement device, suspension and porous material analysis, etc., can solve the problems of difficult volume measurement, cumbersome operation, unfavorable sample detection, etc.

Active Publication Date: 2020-08-14
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that there is down-regulation or abnormal subcellular distribution of AQP5 in the salivary gland cells of Sjogren's syndrome patients and Sjögren's syndrome model mice, which leads to the imbalance of cellular water permeability
At present, existing studies have found that the hypotonic environment is easy to cause some changes in cell morphology, which makes volume measurement difficult under traditional fluorescence microscopes or confocal microscopes, cumbersome operations, and is not conducive to the detection of large quantities of samples.

Method used

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  • A method for detecting the influence of cigarette smoke on cellular water permeability
  • A method for detecting the influence of cigarette smoke on cellular water permeability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for detecting the influence of cigarette smoke on cell water permeability, comprising the steps of:

[0037] Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying mainstream smoke exposure in the oral cavity, using DMEM / F12 cell culture medium to infiltrate the simulated artificial oral mucosa inner wall of the device; select 10 weight A cigarette consistent with the suction resistance is used as the sample to be tested. After the balance, the automatic rotary smoking machine is used for suction. After the suction is completed, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; The equilibrium conditions are: temperature 21°C, humidity 58%, time 48h. The suction frequency of the suction is 1 puff / min, the suction duration is 2 s, and the suction volume is 34.85 mL / puff.

[0038] In step (2), the salivary gland adenoid cystic carcinoma cells were digested wit...

Embodiment 2

[0047]A method for detecting the influence of cigarette smoke on cell water permeability, comprising the steps of:

[0048] Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying mainstream smoke exposure in the oral cavity, using 10mL DMEM / F12 cell culture medium to infiltrate the simulated artificial oral mucosa inner wall of the device; select 20 The cigarette with the same weight and suction resistance is used as the sample to be tested, and after being balanced, it is sucked by a fully automatic rotary smoking machine. After the suction is completed, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; The equilibrium conditions are: temperature 23°C, humidity 62%, time 48h. The suction frequency of the suction is 1 puff / min, the suction duration is 2 s, and the suction volume is 35.15 mL / puff.

[0049] In step (2), the salivary gland adenoid cystic carcinoma cells were d...

Embodiment 3

[0059] A method for detecting the influence of cigarette smoke on cell water permeability, comprising the steps of:

[0060] Step (1), using a bionic absorption device with patent application number 201520354840.8 for studying mainstream smoke exposure in the oral cavity, using 8mL DMEM / F12 cell culture medium to infiltrate the simulated artificial oral mucosa inner wall of the device; select 15 The cigarette with the same weight and suction resistance is used as the sample to be tested, and after being balanced, it is sucked by a fully automatic rotary smoking machine. After the suction is completed, the liquid in the bottle of the bionic absorption device is taken out to obtain the bionic suction liquid; The equilibrium conditions are: temperature 22°C, humidity 60%, time 48h. The suction frequency of the suction is 1 puff / min, the suction duration is 2 s, and the suction volume is 35 mL / puff.

[0061] In step (2), the salivary gland adenoid cystic carcinoma cells were dige...

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Abstract

The invention relates to a detection method for influence of cigarette smoke on cell water permeability, which belongs to the technical field of biological application. A biochemical and cytology method is adopted, BMP6 is used for processing cells, a cell micro environment when a sjogren syndrome occurs is simulated, so that the influence of the cigarette smoke on the water permeability is researched, and compared with the animal experiment, the detection method is simpler and more efficient; an oral bionic simulation apparatus is adopted for trapping the cigarette smoke, thereby being closerto the actual human smoking state; and meanwhile, by adopting a high content system, the influence of a plurality of samples on the cell water permeability can be simultaneously detected at differenttime, the multi-sample detection can be rapidly, accurately and visibly performed, and a novel rapid means can be provided for the efficacy evaluation of the cigarette smoke.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a method for detecting the influence of cigarette smoke on the water permeability of cells. Background technique [0002] Saliva is the main factor to maintain the balance of the oral micro-ecological environment. It has many functions such as moistening the mouth and food, facilitating speaking and swallowing, removing food particles on the taste buds, so as to continuously taste the taste of food, cleaning and protecting the oral cavity. The sensory evaluation of cigarettes shows that some cigarette smoke can cause negative feelings such as dry mouth, dryness, and discomfort. Therefore, the development of cigarette products with the function of reducing dryness and promoting body fluids is of great significance for improving oral comfort and smoking quality of cigarettes. [0003] The aquaporin family (Aquaporins, AQP) is a group of proteins that spe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/08
CPCG01N15/08
Inventor 高茜管莹曾婉俐米其利夭建华张涛朱洲海陆舍铭徐玉琼
Owner CHINA TOBACCO YUNNAN IND
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