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Preparation method of composite hydrogel, and construction method of cell microenvironment bionic system

A technology of composite hydrogel and cell culture, which is applied in the construction of cell microenvironment bionic system and the preparation of composite hydrogel, which can solve the problem that three-dimensional scaffold is difficult to simulate cell microenvironment.

Pending Publication Date: 2021-09-10
NORTHWESTERN POLYTECHNICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The main purpose of the present invention is to provide a method for preparing a composite hydrogel and a method for constructing a cell microenvironment bionic system, so as to solve the problem in the prior art that it is difficult to simulate a real and complex cell microenvironment using a three-dimensional scaffold with a single physical variable

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  • Preparation method of composite hydrogel, and construction method of cell microenvironment bionic system
  • Preparation method of composite hydrogel, and construction method of cell microenvironment bionic system
  • Preparation method of composite hydrogel, and construction method of cell microenvironment bionic system

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Embodiment Construction

[0022] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments of the present invention and corresponding drawings. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] As a kind of extremely hydrophilic three-dimensional network structure material, hydrogel has biophysical properties very similar to natural extracellular matrix, and has been widely used to construct tissue engineering scaffolds. As a natural polysaccharide, sodium alginate has good degradability and biocompatibility, and can quickly cross-link with divale...

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Abstract

The invention discloses a preparation method of composite hydrogel, and a construction method of a cell microenvironment bionic system. The preparation method of the composite hydrogel comprises the following steps: mixing a gelatin solution, a sodium alginate solution, a glycerin solution, a glutaraldehyde solution, a phosphate buffer salt solution and a sterile aqueous solution according to a preset volume ratio to obtain a precursor solution the glycerol volume concentration being 1-5%; freezing the precursor solution to convert gelatin from sol to gel, and freezing glycerol to form pores; and adding a calcium chloride solution to carry out ionic crosslinking on sodium alginate in the gel to obtain the composite hydrogel, wherein the concentration of calcium chloride is associated with the rigidity of the composite hydrogel. The composite hydrogel prepared by the embodiment of the invention is good in forming, stable in mechanical property and uniform in pore structure, and can be used for simulating an extracellular matrix microenvironment in vitro.

Description

technical field [0001] The invention relates to the technical field of biological materials and tissue engineering, in particular to a method for preparing a composite hydrogel and a method for constructing a cell microenvironment bionic system. Background technique [0002] The extracellular matrix is ​​a three-dimensional network composed of extracellular macromolecules, which is the living environment of cells and can provide structural and biochemical support for cells. At present, in vitro cell culture mostly adopts two-dimensional culture with simple operation and low cost, which is far from the complex three-dimensional structure in vivo. Three-dimensional cell culture technology can well construct and simulate the cell-cell and cell-microenvironment intercellular space. interaction. Cells can perceive the biochemical and physical stimuli in their microenvironment and respond to the stimuli, such as adhesion, migration, proliferation, differentiation, and metabolism....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J3/24C08L89/00C08L5/04C08K5/053C08K5/07C08K3/16C12N5/00
CPCC08J3/075C08J3/246C12N5/0062C08J2389/00C08J2405/04C08K5/053C08K5/07C08K2003/162C12N2513/00C12N2533/74C12N2533/54
Inventor 杨慧许琳牧张盼张弩
Owner NORTHWESTERN POLYTECHNICAL UNIV
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