Preparation method of composite hydrogel, and construction method of cell microenvironment bionic system

Abstract

The invention discloses a preparation method of composite hydrogel, and a construction method of a cell microenvironment bionic system. The preparation method of the composite hydrogel comprises the following steps: mixing a gelatin solution, a sodium alginate solution, a glycerin solution, a glutaraldehyde solution, a phosphate buffer salt solution and a sterile aqueous solution according to a preset volume ratio to obtain a precursor solution the glycerol volume concentration being 1-5%; freezing the precursor solution to convert gelatin from sol to gel, and freezing glycerol to form pores; and adding a calcium chloride solution to carry out ionic crosslinking on sodium alginate in the gel to obtain the composite hydrogel, wherein the concentration of calcium chloride is associated with the rigidity of the composite hydrogel. The composite hydrogel prepared by the embodiment of the invention is good in forming, stable in mechanical property and uniform in pore structure, and can be used for simulating an extracellular matrix microenvironment in vitro.

Description

technical field [0001] The invention relates to the technical field of biological materials and tissue engineering, in particular to a method for preparing a composite hydrogel and a method for constructing a cell microenvironment bionic system. Background technique [0002] The extracellular matrix is ​​a three-dimensional network composed of extracellular macromolecules, which is the living environment of cells and can provide structural and biochemical support for cells. At present, in vitro cell culture mostly adopts two-dimensional culture with simple operation and low cost, which is far from the complex three-dimensional structure in vivo. Three-dimensional cell culture technology can well construct and simulate the cell-cell and cell-microenvironment intercellular space. interaction. Cells can perceive the biochemical and physical stimuli in their microenvironment and respond to the stimuli, such as adhesion, migration, proliferation, differentiation, and metabolism....

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Problems solved by technology

[0003] The main purpose of the present invention is to provide a method for preparing a composite hydrogel and a method for constructing a cell microenvironment bionic system, so as to solve the problem in the prior art that it is difficult to simulate a real and complex cell microenvironment using a three-dimensional scaffold with a single physical variable

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