Active oxygen content automatic detection system suitable for cell microenvironment

Abstract

The invention relates to a biochemical detection instrument. The invention aims to provide a rapid, accurate and automatic detection system for realizing the trace volume of a large number of ROS content samples aiming at cell, tissue and organ microenvironments such as pleural fluid, peritoneal fluid, cerebrospinal fluid, amniotic fluid, follicular fluid, cell culture fluid and the like. The technical scheme is as follows: the active oxygen content automatic detection system suitable for a cell microenvironment is characterized in that the automatic detection system comprises a sample transmission reaction system, a detection system, a washing system communicated with the sample transmission reaction system through a water pipe pipeline and a purging system communicated with the sample transmission reaction system through an air pipe pipeline, wherein the sample transmission reaction system and the detection system are sequentially communicated through a light shielding pipeline; thesample transmission reaction system comprises a sample injector and a DCFH supply bin which are connected in parallel and then communicated with a reaction bin through a light shielding pipeline; andsample injection valves are respectively arranged between the sample injector and the reaction bin and between the DCFH supply bin and the reaction bin.

Description

technical field [0001] The invention relates to a biochemical detection instrument, in particular to an instrument for automatically detecting the content of active oxygen in the microenvironment trace liquid of cells, tissues and organs. Background technique [0002] Reactive oxygen species (reactive oxygen species, ROS) refers to in aerobic organisms, the oxygen entering the mitochondria is converted into peroxides and oxygen-containing free radicals with strong reducing and strong oxidizing properties through metabolic processes. Common reactive oxygen species include hydrogen peroxide (H 2 o 2 ), hypochlorous acid / hypochlorite ion (HClO / ClO - ), peroxynitrite ion (ONOO - ),Hydroxyl radicals( · OH), nitric oxide (NO · ), superoxide anion (O 2 ·- ), lipid peroxidation free radicals (ROO · ). ROS are constantly produced in the body and will be constantly cleared by some antioxidants in the body, maintaining a dynamic balance level and affecting various physiologica...

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Problems solved by technology

[0005] In the detection of ROS based on chemiluminescence, colorimetry, fluorescence and electrochemical fluorescence, commonly used instruments include flow cytometer, multi-functional microplate reader, fluorescence microscope, etc., because ROS in the sample is unstable when exposed to air, However, the existing detection methods all need to go through multiple steps such as probe loading, incubation, washing, and loading on the machine. Therefore, it is difficult for the above-mentioned instruments to achieve the purpose of rapid, accurate, and automatic detection of ROS in a large number of samples, so there is no such method in existing clinical tests. Available ROS level detection methods

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