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Methods for therapeutics prescreening in bodily fluids

A therapeutic agent, carrier technology, used in the field of trials to predict the efficacy of personalized treatment and the activity of the therapeutic agent, which can solve the problems of expensive, missed, and patient invasive for caregivers

Pending Publication Date: 2020-04-21
BARCODE DIAGNOSTICS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such frequent extractions in the form of biopsies are invasive for the patient, expensive for the caregiver, and sometimes impossible depending on the location of the disease
Further, cell extraction runs the risk of sampling only some cells of the disease and missing others

Method used

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  • Methods for therapeutics prescreening in bodily fluids
  • Methods for therapeutics prescreening in bodily fluids
  • Methods for therapeutics prescreening in bodily fluids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0173] Example 1: Equal numbers of liposomes with equal relative drug concentrations are required for accurate evaluation of efficacy

[0174] Experiments were performed to test the ideal dose of each drug included in the liposome mixture for theranostic applications. Liposomes loaded with doxorubicin (barcode 2), gemcitabine (barcode 3) or cisplatin (barcode 4) were generated according to pre-existing protocols (see International Patent Publication WO2016024281, incorporated herein by reference in its entirety). For each batch of liposomes, lipid concentration, encapsulated drug concentration, size distribution, and zeta potential were measured. The results are summarized in Table 1. Because all the different batches were liposomes with the same lipid composition (55 mol% HSPC, 40 mol% cholesterol, and 5 mol% DSPE-PEG) and size (about 100 nm), it was assumed that the lipid concentration (assessed by Stewart test ) and the derivatized amount of lipid (in moles) is proportion...

Embodiment 2

[0183] Example 2: Theranostic Assays in Fluid Biopsies

[0184] Using the method of generating liposome mixtures, which yielded more accurate results at hand, it was hypothesized that barcoding not only tumor biopsies but also cell-free tumor DNA (cftDNA) circulating in the bloodstream could be performed. The mouse 4T1 mammary tumor model was used to test the feasibility of liposomal barcoding in blood for theranostic applications. 4T1 breast cancer cells were grown under standard tissue culture conditions. Mix 50 μl of 6x10 6 cells / ml of 4T1 cells (3x10 5 cells / mouse) subcutaneously into the left anterior papilla of 8-week-old BALB / c female mice. Animal weight and tumor size (length and width) and body condition were recorded every 2-3 days starting from injection. Tumor volume was measured with calipers and calculated as length / 2×(width) 2 .

[0185] Three types of liposomes were tested together in the mixture: doxorubicin-containing (barcode 2), gemcitabine-containing...

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Abstract

A method for using bodily fluids in determining the cell-specific potency of drugs is provided. A composition comprising a plurality of types of carriers, wherein each carrier comprises a single-celllethal amount of a therapeutic agent and a unique barcode identifying that agent and wherein the composition comprises equal numbers of each carrier type, equal concentrations of the agent and equal concentrations of each barcode is also provided. Further, methods useful for studying the therapeutic profile of one or more drugs within a cell microenvironment, including but not limited to a tumor,are provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 529,023, filed July 6, 2017, and entitled "METHODS FOR THERAPEUTICSPRESCREENING IN BODILY FLUIDS," the contents of which are incorporated herein by reference in their entirety. technical field [0003] The present invention relates to assays that can be used to predict the efficacy of personalized treatments and the activity of therapeutic agents. Background technique [0004] Patients respond differently to drug therapy; treatment success depends greatly on choosing the right drug for each patient. "Personalized Medicines" aims to address each patient's unique disease presentation. Diseases and disorders requiring personalized medicine include malignant, inflammatory, and neurodegenerative diseases. [0005] Cancer, as a non-limiting example, has a low actual response rate to drugs in the clinic. For example, fewer than 60% of patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/6886
CPCC12Q1/68C12Q1/6886C12Q2600/106C12Q2600/136C12Q2563/185G01N2800/52A61K49/0017A61K49/00
Inventor R·伊夫里A·萨巴F·巴丁特R·图瓦A·施罗德
Owner BARCODE DIAGNOSTICS LTD
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