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Vasculation bone cell membrane sheet stent complex and preparation thereof

A cell membrane, complex technology, applied in the direction of vascular endothelial cells, bone/connective tissue cells, cell culture support/coating, etc. , the effect of maintaining the osteogenic space

Pending Publication Date: 2022-02-15
STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the shortcomings of osteogenesis and vascularization and the low strength of cell membranes in traditional tissue engineering, and provides a method for constructing a vascularized osteoblast membrane scaffold complex

Method used

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  • Vasculation bone cell membrane sheet stent complex and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of a vascularized osteoblast membrane scaffold complex

[0032] 1. Medium

[0033] Osteogenic Differentiation Medium (OM):

[0034] 50nM dexamethasone, 5mM β-glycerophosphate, 10μg / mL L-ascorbic acid, the balance is proliferation medium (including 10% fetal bovine serum, 1% penicillin-streptomycin mixed solution, the balance is Dulbecco's modified Eagle medium - high glucose).

[0035] Endothelial Cell Medium (ECM):

[0036] Produced by ScienceCell, it contains the following components: 500mL basal medium, 25mL fetal bovine serum, 5mL endothelial cell growth factor, 5mL penicillin-streptomycin solution.

[0037] 2. Preparation method

[0038] (1) Orifice plate co-cultivation: human periodontal ligament mesenchymal stem cells and human umbilical vein endothelial cells were mixed according to the quantitative ratio of 3:1, 2:2, and 1:3 respectively, and the volume ratios of OM and ECM were respectively 3:1, 2:2, 1:3 mixed media were cultured for...

Embodiment 2

[0041] Embodiment 2 performance test

[0042] The performance test was performed on the cell sheet prepared according to the ratio of human periodontal ligament-derived mesenchymal stem cells and human umbilical vein endothelial cells at a ratio of 3:1 in Example 1, and the control 1 cell sheet (4:0).

[0043] 1. Experimental method

[0044] The acquired vascularized osteoblast membrane sheet and the control cell membrane sheet were wrapped on a β-TCP scaffold with a size of 5 mm × 10 mm × 15 mm, and 6 samples of each ratio of cell membrane TCP scaffold complex were placed on a ratio consistent with the size of the scaffold. The mandibular alveolar bone defect of the dog was fixed with titanium nails, covered with Bio-Gide collagen membrane, and sutured in layers. Three months after the operation, a small amount of mandibular alveolar bone samples were scraped, fixed with 4% paraformaldehyde, and sent for MicroCT scanning. Among them, blank refers to the mandibular alveolar ...

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Abstract

According to the vascularization bone cell sheet scaffold complex and the preparation method thereof, a method for co-culturing osteoblast cells and angiogenic cells is adopted, through a cell sheet technology, the loss of extracellular matrixes caused by a traditional cell digestion technology is eliminated, the original cell microenvironment is reserved, and the cell sheet scaffold complex has the advantages that the cell sheet scaffold complex is simple in structure and convenient to use. Through the synergistic effect of osteogenesis and vascularization, the survival and outcome of osteoblasts in the bone tissue engineering scaffold are facilitated; furthermore, the organic combination of the 3D printing bone tissue engineering scaffold and the cell sheet solves the problems that a pure cell sheet is low in strength and cannot maintain an osteogenesis space.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a vascularized osteoblast membrane scaffold complex and its preparation. Background technique [0002] Tumors, trauma, infection, and congenital deformities often lead to severe maxillofacial bone defects, which affect the function and appearance of patients. Autologous bone grafting is the "gold standard" for testing the repair of bone defects, but it has disadvantages such as large trauma and insufficient bone in the donor site. At present, tissue engineered bone constructed by seed cells and 3D printing can repair complex bone defects in a non-invasive, safe and personalized manner, and has a good application prospect. [0003] Currently available seed cells for bone tissue engineering include adult stem cells, embryonic stem cells and induced pluripotent stem cells. Theoretically, embryonic stem cells have the ability to differentiate into three germ layers and h...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61F2/28
CPCC12N5/0654A61F2/28A61F2002/2817C12N2506/1346C12N2506/28C12N2513/00C12N2533/20
Inventor 周苗魏志斌郭凯李树祎耿远明
Owner STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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