Isolated culture method for tea leaf suspension single cells

A separation culture and suspension culture technology, applied in the field of plant tissue culture, can solve the problems of lack of cytology platform, self-recession, hindering tea and other problems

Active Publication Date: 2017-08-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high degree of self-incompatibility, self-recession, and low seed setting rate of tea plants limit the genetic improvement work; in addition,

Method used

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  • Isolated culture method for tea leaf suspension single cells
  • Isolated culture method for tea leaf suspension single cells
  • Isolated culture method for tea leaf suspension single cells

Examples

Experimental program
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Embodiment

[0030] (1) Preparation of solid callus induction medium and solid callus subculture medium:

[0031] Take the preparation of 1L medium as an example, weigh the MS medium mother liquid prepared in advance according to the proportion, put it in a 5L beaker, add 2,4-D at a ratio of 2.0mg / L, and add KT at a ratio of 0.5mg / L , Add sucrose at a ratio of 30g / L, add agar at a ratio of 7g / L, add to boiling pure water and stir evenly, pour into a 5L beaker to the scale of 1L, and stir evenly, then adjust the pH with 1mol / L sodium hydroxide to 5.8, then divided into tissue culture bottles, sterilized under high pressure at 121°C for 15 minutes, fully cooled and solidified, and set aside to obtain a solid callus induction medium.

[0032] Take the preparation of 1L medium as an example, weigh the MS medium mother liquid prepared in advance according to the proportion, put it in a 5L beaker, add 2,4-D at a ratio of 1.0mg / L, and add KT at a ratio of 0.1mg / L , Add sucrose at a ratio of 30g / ...

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Abstract

The invention discloses an isolated culture method for tea leaf suspension single cells. The method comprises the following steps: selecting tea leaves; disinfecting aseptic leaves; inducing healing tissues; culturing liquid suspension cells; performing enlarged culture on the liquid suspension cells; separating the single cells and the like. The tea leaf suspension cells prepared by the method have high growing speed and higher metabolism activity. A rapid-growing suspension cell culture system and a single cell separating method are provided, so that a basis is laid for tea leaf cytological studies and researches of secondary metabolites. A good technical platform is provided for subsequent production of tea tree secondary metabolites, metabolite cellular localization, metabolic mechanism study as well as tea tree cell metabolite change and mechanism study under the stress condition of exogenous compounds.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for obtaining tea leaf suspension single cells by separating single cells from the callus after the plant tissue culture method is used to obtain the callus tissue of tea tree leaves. Background technique [0002] Tea tree, Latin name: Camellia sinensis (L.) O.Ktze, Camellia, Camellia shrub or small tree, twigs glabrous. Leaves leathery, oblong or elliptic. The leaves of the tea tree can be used to make tea (different from the tea tree), the seeds can be used to extract oil, the material of the tea tree is fine and dense, and its wood can be used for carving. [0003] my country is a traditional big exporter of tea. According to relevant data, among all tea-producing countries in the world, my country ranks first in terms of annual tea output and second in total export volume, only lower than Kenya. Tea contains tea polyphenols, amino acids, caffeine and o...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/04C12N2500/30C12N2500/34C12N2509/00
Inventor 侯如燕胡奕铮焦卫婷孙俊李建超肖昱
Owner ANHUI AGRICULTURAL UNIVERSITY
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