Preparation method and application of liposomes of shikonin and derivatives or modifiers thereof

Abstract

The invention belongs to the fields of pharmaceutical engineering and biotechnology, particularly relates to a prescription process for preparing a shikonin liposome and an effect of the shikonin liposome on resisting generation of new blood vessels. A human umbilical vein endothelial cell (HUVEC) strain is adopted in an anti-angiogenic test, and inhibition (MTT) on a proliferation effect thereof and inhibition (Transwell) on a migration effect thereof are measured. In addition, the invention also relates to shikonin cysts in liposomes, bright red fluorescent light can be observed under the excitation of green fluorescent light, and the shikonin cysts have important application values in the aspects of encapsulation rate measurement, in-vitro cellular localization, endocytosis mechanisms, in-vivo drug tracking and the like. After the shikonin liposomes act on the HUVEC or other tumor cells for a period of time, a confocal microscopy can be used for carrying out cellular fluorescent localization.

Description

Liposome preparation method and application of shikoning and its derivatives or modifications technical field The invention belongs to the field of pharmaceutical engineering and biotechnology, and specifically relates to the preparation of shikonin liposome and its anti-angiogenesis effect; The bright red fluorescence phenomenon can be observed under the environment, so it has important application value in the determination of encapsulation efficiency, in vitro cell localization, cell entry mechanism, and in vivo drug tracking. Background technique Shicaoning is an active ingredient isolated from the root of the Chinese medicinal material Comfrey, which was mainly used in the treatment of macule, measles, carbuncle, burns and other diseases in ancient times (Huang et al., 2002). Modern studies have shown that it has a variety of pharmacological activities, such as anti-inflammatory, anti-oxidation, anti-gonadotropin, anti-bacterial, anti-angiogenesis, anti-HIV, anti-cell...

AI Technical Summary

Problems solved by technology

However, due to the low solubility and strong cytotoxicity of shikoning, it has not been used clinically

Images