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Flow cytometry electrofusion apparatus

A technology of flow cytometry and cell fusion, which is applied in the field of biomedicine, can solve problems such as the inability to achieve cell fusion, and achieve the effect of facilitating follow-up work and high matching rate

Inactive Publication Date: 2013-06-05
BEIHANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the existing cell fusion technology and equipment cannot achieve a large number of specific cell fusion

Method used

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Embodiment Construction

[0023] Such as figure 1 , 2 , 3, the present invention includes a cell one-to-one fusion part 1, the cell one-to-one fusion part 1 is an integral chip, and a hollow and airtight cell fusion chamber 2 is arranged in the integral chip. The cell fusion chamber 2 has a width of 30-60 μm, a height of 30-60 μm, and a length of 2 cm. The two ends of the cell fusion chamber 2 are respectively a first cell injection hole 6 and a second cell injection hole 7 . A plurality of parallel cell positioning channels 3 are distributed on the bottom of both sides of the cell fusion chamber 2 . A plurality of parallel cell positioning channels 3 are located at the bottom of both sides of the cell fusion chamber 2 at an interval of 80-100 μm, and the cross section of each positioning channel is a square with a side length of 3-10 μm, and the channel length is 100-200 μm.

[0024] The cell positioning channel 3 communicates with the cell absorption channel 4. The end of the cell absorption chann...

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Abstract

The invention discloses a flow cytometry electrofusion apparatus. The flow cytometry electrofusion apparatus comprises: a one-to-one cell fusion portion, a fusion cell detecting and sorting portion and a single cell collection portion. The one-to-one cell fusion portion is provided with a plurality of parallel cellular localization channels. Different cells can be adsorbed to ends of the channels, and two different cells can close to each other and be fused. The fused cells are placed until membranes of the different cells are completely fused, and then are put into the fusion cell detecting and sorting portion. The fusion cell detecting and sorting portion comprises a laser light source, a fluorescence detector and a deflector. The fused cells, i.e., red and green double fluorescent stained cells, are collected in a collecting tube. When collecting a fused cell, flow of droplet in the fluorescence detector is stopped. 200 [mu]l of cell culture medium is added to the collecting tube through a cell culture medium accumulator installed above the collecting tube to open the end of the collecting tube. The 200 [mu]l of the cell culture medium containing the fused cell flows into a well of a 96-well plate arranged blow the collecting tube. At this time, the flow of the liquid in a flow chamber is started, and the next fused cell is put into the next well of the 96-well plate. According to the present invention, one-time continuous accomplishment of one-to-one cell fusion can be realized, fusants can be detected correctly after fusing and each fusant can be cultured respectively.

Description

technical field [0001] The invention relates to a flow cell electrofusion instrument, which belongs to the technical field of biomedicine. Background technique [0002] Electrofusion technology is a new fusion-promoting technology established in the 1980s. In 1981, Zimmerman invented the electrofusion instrument and proposed the concept of electrofusion for the first time; in 1987, Schweiger established a single-pair protoplast electrofusion technology procedure. The basic principle is to place the mixture of two kinds of cells in a low-voltage AC electric field to make the cells aggregate into a beaded shape, and then apply high-voltage electric pulses to promote cell fusion. The main purpose of cell fusion is to study the influencing factors of gene recombination after heterogeneous cell fusion, produce hybridoma cells, and plant and microbial cell hybrid breeding. After decades of development, electrofusion technology has matured day by day, and its many advantages have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12N15/02
CPCC12M35/02
Inventor 樊瑜波孙艳崔绍艳王玮刘姚萍
Owner BEIHANG UNIV
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