Method for preparing bifluorescence emission nano-probes in post-encoding mode

A technology of nanoprobes and fluorescent probes, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of time-consuming, affecting codons, large codon requirements, etc., and achieve biocompatibility Good, good biocompatibility, good reproducible results

Active Publication Date: 2012-10-03
NANKAI UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the process of preparing probes in the pre-coding method is quite time-consuming, and the change of the amount of coding elements will serio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing bifluorescence emission nano-probes in post-encoding mode
  • Method for preparing bifluorescence emission nano-probes in post-encoding mode
  • Method for preparing bifluorescence emission nano-probes in post-encoding mode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] A method for preparing dual fluorescent emission nanoprobes by post-coding, using cadmium telluride quantum dots and gold nanoclusters as fluorescent coding elements, silicon spheres as the carrier of the coding elements, combined with the fluorescent properties of quantum dots and gold nanoclusters, Synthesizing a fluorescent probe precursor that can be used for post-coding, and then using the method of adding an optical regulator L-cysteine ​​to the precursor to prepare a dual fluorescent emission nanoprobe, including the following steps:

[0031] 1) Add 53 μL of mercaptopropionic acid (MPA) to 12.5 mL of CdCl 2 In, CdCl containing mercaptopropionic acid is obtained 2 solution;

[0032] 2) Mix 0.064g tellurium powder (Te), 0.36g NaBH 4 Mix with 8mL of distilled water, and carry out the reduction reaction under the condition of nitrogen protection and stirring to obtain NaHTe solution;

[0033] 3) Preparation of cadmium telluride quantum dot solution: quickly add Na...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing bifluorescence emission nano-probes in a post-encoding mode. A fluorescent probe precursor which can be used for post-encoding is synthesized by taking cadmium telluride quantum dots and gold nanoclusters as fluorescence encoding elements and silicon balls as a carrier of the encoding elements according to the fluorescent characteristics of the quantum dots and the gold nanoclusters; and then an optical regulator is added into the precursor in a post-encoding mode to prepare the bifluorescence emission nano-probes. The fluorescence intensity ratio of two encoding elements in the obtained bifluorescence emission nano-probes is relatively large, so that the observation on the change of a fluorescent signal at relatively high resolution is facilitated. The method for preparing the bifluorescence emission nano-probes comprises the following steps of: preparing the quantum dots, wrapping silicon on the quantum dots, synthesizing the gold nanoclusters, synthesizing fluorescent probe precursor microspheres, and preparing the bifluorescence emission nano-probes. The invention has the advantages that the synthetic method is simple; the encoded probes have the irreversibility; the reproducibility of different batches of probes is high; and an effective method for preparing a large number of bifluorescence emission nano-probes which can be used for fluorescent imaging is provided.

Description

technical field [0001] The invention relates to a preparation technology of a double fluorescence emission nano-probe, in particular to a method for preparing a double fluorescence emission nano-probe in a post-coding manner. Background technique [0002] In the field of biotechnology, the development of high-throughput imaging and multicolor flow counters has promoted the preparation of high-capacity fluorescent carriers and nanoprobes. When multivariate analysis, such as multicolor labeling and multicolor imaging, has a large demand for imaging codons, it is necessary to develop a novel probe encoding strategy to meet this urgent demand. There are currently two coding strategies, pre-coding and post-coding. Because the process of preparing probes in the pre-coding method is quite time-consuming, and the change of the amount of coding elements will seriously affect the smooth progress of the preparation process of codons, it cannot meet the problem of large demand for codo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09K11/88G01N21/64
Inventor 李文友王艳芹何锡文张玉奎
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap