Method for preparing bifluorescence emission nano-probes in post-encoding mode

Abstract

The invention discloses a method for preparing bifluorescence emission nano-probes in a post-encoding mode. A fluorescent probe precursor which can be used for post-encoding is synthesized by taking cadmium telluride quantum dots and gold nanoclusters as fluorescence encoding elements and silicon balls as a carrier of the encoding elements according to the fluorescent characteristics of the quantum dots and the gold nanoclusters; and then an optical regulator is added into the precursor in a post-encoding mode to prepare the bifluorescence emission nano-probes. The fluorescence intensity ratio of two encoding elements in the obtained bifluorescence emission nano-probes is relatively large, so that the observation on the change of a fluorescent signal at relatively high resolution is facilitated. The method for preparing the bifluorescence emission nano-probes comprises the following steps of: preparing the quantum dots, wrapping silicon on the quantum dots, synthesizing the gold nanoclusters, synthesizing fluorescent probe precursor microspheres, and preparing the bifluorescence emission nano-probes. The invention has the advantages that the synthetic method is simple; the encoded probes have the irreversibility; the reproducibility of different batches of probes is high; and an effective method for preparing a large number of bifluorescence emission nano-probes which can be used for fluorescent imaging is provided.

Description

technical field [0001] The invention relates to a preparation technology of a double fluorescence emission nano-probe, in particular to a method for preparing a double fluorescence emission nano-probe in a post-coding manner. Background technique [0002] In the field of biotechnology, the development of high-throughput imaging and multicolor flow counters has promoted the preparation of high-capacity fluorescent carriers and nanoprobes. When multivariate analysis, such as multicolor labeling and multicolor imaging, has a large demand for imaging codons, it is necessary to develop a novel probe encoding strategy to meet this urgent demand. There are currently two coding strategies, pre-coding and post-coding. Because the process of preparing probes in the pre-coding method is quite time-consuming, and the change of the amount of coding elements will seriously affect the smooth progress of the preparation process of codons, it cannot meet the problem of large demand for codo...

AI Technical Summary

Problems solved by technology

Because the process of preparing probes in the pre-coding method is quite time-consuming, and the change of the amount of coding elements will seriously affect the smooth progress of the preparation process of codons, it cannot meet the problem of large demand for codons in multivariate analysis.

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