40 results about "Thrombin aptamer" patented technology

The invention discloses preparation and application of a thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod. The thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod is prepared through the following steps of: synthesizing a polyacrylonitrile-maleic acid polymer by adopting a free radical water-phase precipitation polymerization method; then preparing a solid-phase extraction rod which has the advantages of large surface area, high stability and high durability by directly taking a stainless steel wire as a substrate and adopting an electrospining technology; and fixing a thrombin aptamer to the surface of the solid-phase extraction rod by adopting a carbodiimide method. The prepared thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod can be applied to the isolation analysis of a trace amount of thrombin contained in complex samples, such as human serum, human plasma, human blood, and the like. The method disclosed by the invention can be used for preparing a solid-phase microextraction base body coating and controlling the thickness of the solid-phase microextraction base body coating by taking the stainless steel wire as the substrate and adopting an electrospining technology; the solid-phase microextraction base body coating is uniform, loose, porous and large in surface area; and the prepared solid-phase microextraction rod has the advantages of high specificity and high stability.

The invention relates to a preparation method and application of a photoelectrochemical thrombin aptamer sensor based on PS@Au dual inhibition of ZnCdS, belonging to the field of photoelectrochemical sensors. Zinc and cadmium co-doped sulfide ZnCdS nanocrystals were synthesized by a simple hydrothermal method. Its rough surface and large specific surface area greatly increased the loading capacity of ZnCdS and enhanced its visible light absorption capacity. Using ZnCdS as the substrate material and PS@Au as the secondary antibody label, PS@Au has good insulation, which hinders the transfer of photogenerated electrons, and the competition for the absorption of visible light between PS@Au and ZnCdS will also effectively reduce the light current signal, this dual suppression effect effectively improves the sensitivity of the sensor and reduces the detection limit of the sensor. Combined with the excellent selectivity of the appropriate ligand, the highly sensitive detection of the thrombin aptamer is realized, which is of great significance for the analysis and detection of thrombin.

A carbon nanotube microcantilever biosensor is used to detect thrombin with a concentration range of 0.5-10µg / mL, which is realized by constructing a carbon nanotube microcantilever biosensor. The biosensor includes a support, a base material, a carbon nanotube, and a pick-up circuit, and a layer of nucleic acid aptamer is modified on the carbon nanotube through π-π superposition. First, a detection probe containing a thrombin nucleic acid aptamer is made on the carbon nanotube micro-cantilever beam. During detection, the detection probe is put into the sample to be tested, and the thrombin in the sample to be tested reacts with the detection probe through a specific reaction. The nucleic acid aptamer on the microcantilever forms a complex and attaches to the microcantilever; the mass change generated by the complex on the microcantilever causes the flexural displacement or the change of the resonance frequency of the microcantilever and the relationship between the mass size of the complex and the The concentration of thrombin in the sample to be tested is positively correlated, thereby realizing the detection of thrombin.

The application discloses a thrombin detection method and a kit thereof, the method comprising the following steps: 1) synthesizing a complementary PNA chain according to the DNA sequence of the thrombin aptamer; 2) combining the DNA sequence of the thrombin aptamer with The PNA chain synthesized in the step 1) is mixed to prepare a substrate solution; 3) adding a chromogen to the substrate solution to observe the color of the solution; 4) adding the sample to be tested, observing the color of the solution, and judging the The concentration of thrombin in the sample to be tested. The detection method of the present application is fast and convenient, has extremely low requirements on instruments and equipment, and has high sensitivity and good specificity.

The invention relates to a Ti-based 3 C 2 A thrombin aptamer sensor and a preparation method thereof belong to the technical field of biological analysis and detection. It solves the technical problems that the antibodies used in traditional thrombin detection methods are difficult to prepare and store, and the related reactions are complicated and time-consuming. The Ti-based 3 C 2 The thrombin aptamer sensor is composed of monolayer Ti 3 C 2 and its binding to Ti 3 C 2 The composition of the fluorescein-labeled thrombin aptamer on the surface; the sequence of the fluorescein-labeled thrombin aptamer is: 5′-FAM-GGT TGG TGT GGT TGG-3′. The present invention uses Ti 3 C 2 Material builds thrombin sensor, broadens Ti 3 C 2 In the application in the biological field, an excellent nano-detection platform has been constructed; the thrombin aptamer is used as the thrombin recognition agent, which is cheap, easy to synthesize, easy to store, and fast in response compared with traditional antibodies. The invention provides the Ti-based 3 C 2 The thrombin aptamer sensor has the characteristics of high specificity and high sensitivity, and the detection limit of thrombin is 2.5×10 ‑11 mol / L.

The invention discloses an isothymidine-modified thrombin nucleic-acid aptamer, and a preparation method and application thereof, and belongs to the field of biomedicine. By doping isothymidine shown as a chemical formula I and / or II into synthesized thrombin nucleic-acid aptamer, the isothymidine-modified thrombin nucleic-acid aptamer is obtained. Experiments prove that the human thrombin nucleic-acid aptamer modified by employing the method is capable of relatively well recognizing thrombin, is relatively strong in bonding force and has relatively strong anticoagulation effect, and therefore the isothymidine-modified thrombin nucleic-acid aptamer is hopeful to be prepare a high-efficiency high-selectivity anticoagulation medicine with low toxic and side effects.

A titanium carbide three-dimensional composite material, a preparation method therefor, and application thereof in constructing a thrombin aptasensor. A three-dimensional composite material substrate TiO2 / Ti3C2TX is formed by self-growing TiO2 nanorods on the surface of Ti3C2TX with a one-step hydrothermal method, and a three-dimensional composite material M NPs / TiO2 / Ti3C2TX is further obtained by reducing noble metal nanoparticles M NPs on the surface of a three-dimensional material. The composite material has the highest current intensity because a variety of enhancements are achieved in one step. The three-dimensional structure of the composite material can provide a particularly large accessible surface area, which facilitates anchoring of M NPs. The introduction of a transition metal carbonitride MXenes and the metal nanoparticles can improve the efficiency of charge separation and accelerate electron transfer. A sensitive unlabeled aptamer is successfully established by means of the combination of M NPs and aptamer chains, and is used for determining enzyme protein. The provided aptasensor has good electrochemical performance, a wide linear range, and a low detection limit; this indicates that M NPs / TiO2 / Ti3C2TX is promising to be used as an electrode material in an electrochemical biosensor.

The invention relates to a hairpin DNA probe and a method for quantitatively detecting thrombin. A thrombin aptamer is embedded into the hairpin DNA probe, the sequence of the thrombin aptamer refers to the base sequences of 5'-GAATTC TTAAA GGTTGGTGTGGTTG GAATTC-3' and GGTTGGTGTGGTTG G, and a part formed by pairing 5'-GAATTC and 3'-CTTAAG is a stem part of a hairpin DNA stem-loop structure. The method comprises the following steps: in a Tris-HCl buffer solution system, reacting a hairpin DNA solution and thrombin at the constant temperature of 37 DEG C, adding a CTBA solution to react with the previous solution, adding an MB solution, completely mixing and reacting at room temperature, and finally testing the analysis and detection performance of the probe on the thrombin on a fluorospectro photometer. The hairpin DNA probe disclosed by the invention is simple, convenient and high in specificity, can be used for effectively performing quantitative detection on the thrombin and has wide linear range.