40 results about "Thrombin aptamer" patented technology

The invention relates to a photoelectrochemical sensor, and preparation and application thereof. A preparation method for the photoelectrochemical sensor comprises the following steps: S1, pretreating ITO electro-conductive glass; S2, adding TiO2 nanoparticles onto the ITO electro-conductive glass drop by drop to prepare an ITO/TiO2 electrode; S3, soaking the electrode in a carbon quantum dot solution for 10 h so as to obtain an ITO/TiO2/CODs electrode; S4, adding chitosan onto the electrode drop by drop so as to obtain an ITO/TiO2/CODs/CS electrode; S5, adding a glutaraldehyde solution onto the electrode drop by drop; and S6, dropwise adding thrombin aptamer drops, carrying out a reaction at 4 DEG C under a wet condition for 10 to 15 h, continuing adding bovine serum albumin onto the electrode drop by drop after leaching and blow-drying, and carrying out leaching and blow-drying again. The prepared photoelectrochemical sensor can be used for detection of thrombin. According to the invention, the carbon quantum dots with a wide visible light absorption range, good luminosity and high-efficiency electron injection capability are used as a photosensitizer for titanium dioxide, so the obtained composite structure has good response to photoelectric current under visible light. The prepared photoelectrochemical sensor has the advantages of simple operation, low cost, environment friendliness, high sensitivity and good selectivity.

The invention relates to a three-dimensional composite material of the titanium carbide, and a preparation method thereof, and an application in constructing a thrombin aptasensor. A substrate of thethree-dimensional composite material TiO2/Ti3C2TX is formed by the self-growth of TiO2 nanorods on the surface of Ti3C2TX by one-step hydrothermal method; meanwhile, the three-dimensional composite material M NPs/TiO2/Ti3C2TX is obtained by reducing noble metal nanoparticles (M NPs) on the surface of the three-dimensional material. The material has a highest current intensity, due to the fact thata variety of enhancements are achieved in one step. The three-dimensional structure of the composite material may provide a particularly large accessible surface area, which facilitates the anchoringof the M NPs. The introductions of transition metal carbonitrides (MXenes) and metal nanoparticles can improve the efficiency of the charge separation, and accelerate the electron transfer rate. A sensitive unlabeled aptamer is successfully established through the combination of M NPs and aptamer chains, and is used for determining the enzyme protein. According to the three-dimensional compositematerial of the titanium carbide, and the preparation method thereof, and the application in constructing thrombin aptasensor, the aptasensor has a good electrochemical performance, a wide linear range, and a relatively low detection limit, thereby indicating that the M NPs/TiO2/Ti3C2TX will be promising for the use as electrode materials in electrochemical biosensors.

Belonging to the technical field of optical sensing, the invention discloses a thrombin detection method based on bio-dots and Au NPs fluorescence resonance energy transfer. A thrombin aptamer is employed as the template to prepare bio-dots, the bio-dots are mixed with a to-be-tested thrombin sample, then the mixed solution is added into an Au NPs solution, at the moment, the fluorescence intensity of the bio-dots is directly correlated with the concentration of thrombin, and the concentration of thrombin is judged according to the fluorescence intensity of bio-dots.

The invention discloses construction of a portable glucometer-based paper analysis device for thrombin detection. A hydrophobic wax printing pattern of a microfluidic paper chip is designed by an Adobe illustrator CS4 software on a computer; gold nanoparticles grow in a hydrophilic working area of the paper chip, a hairpin-structured probe containing a thrombin aptamer chain is loaded, and a target object is captured; and a sucrose-labeled recognition chain and the hairpin structure opened probe are hybridized, glucose is generated by the catalytic hydrolysis effect of sucrose on cane sugar so as to further realize detection of thrombin through a portable glucometer.

The invention discloses a method detecting harmful compounds in a plastic toy based on a surface plasma resonance technology, belonging to the technical field of residue detection of harmful compounds. The method comprises sensing surface aptamer fixing, sample marking, sample detecting and sensing element regenerating; the same sensing chip is used for detecting the residual contents of different harmful compounds; and the method is characterized by comprising the following steps of: connecting the surface of the sensing chip with a thrombin aptamer serving as a marking molecular probe; performing a series of pretreatment on a sample, adding thrombin markers for detecting harmful compounds, mixing, and competitively binding the aptamers which correspond to meal nanoparticle-modified harmful compounds; detecting the content of thrombin by using a surface plasma resonance detector, and indirectly detecting harmful compounds; and regenerating the sensing chip which is used for detecting other harmful compounds. A general detection method with high stability, high repeatability and high sensitivity is provided for detecting harmful compounds in plastic toys, detection of different harmful compounds with the same surface plasma resonance sensing chip is realized, and high generality is achieved.

The invention relates to a thrombin detection method based on aptamer and telomerase amplification. The method has the advantages that the method targets thrombin, aptamer is used to identify the target, the signal is further amplified through telomerase amplification, and visual thrombin detection is achieved by further using magnetic separation; the method controls reaction conditions, the surface of a magnetic bead is modified with a probe for identifying thrombin, the probe comprises a thrombin aptamer sequence and a telomerase identification sequence, and the telomerase identification sequence at the terminal of the probe is exposed after the probe is combined with the target; the separation method is convenient, all materials are commercially available, and high bio-safety is achieved; the method is simple in reaction, low in cost and sensitive and visual in detection.

The invention relates to a method for detecting thrombin based on a barometer, and belongs to the field of analytical chemistry. The method comprises the steps: first modifying catalase on an aptamer of the thrombin, then modifying another section of aptamer B of the thrombin on a magnetic ball, and taking the two sections of aptamers as a capturing recognition probe. When the thrombin exists, a polymer of a sandwiched structure of magnetic ball/aptamer B-thrombin-aptamerA/CAT can be formed, the polymer can be migrated and concentrated under the action of a magnetic field, so that the polymer is convenient to separate and purify; CAT can catalyze a hydrogen peroxide solution to be decomposed to generate hydrogen, thus causing the variation of air pressure in a closed environment, thereby being suitable for the detection application of the barometer. According to the method, the barometer that is low in price is used for substituting a traditional analytical detection tool as a detection means; the thrombin aptamer is used for efficiently recognizing and capturing the specificity of the target thrombin, and a novel method which is low in detection cost, simple to operate, accurate in quantization result and capable of immediately and rapidly detecting the thrombin is established.

The invention discloses preparation and application of a thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod. The thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod is prepared through the following steps of: synthesizing a polyacrylonitrile-maleic acid polymer by adopting a free radical water-phase precipitation polymerization method; then preparing a solid-phase extraction rod which has the advantages of large surface area, high stability and high durability by directly taking a stainless steel wire as a substrate and adopting an electrospining technology; and fixing a thrombin aptamer to the surface of the solid-phase extraction rod by adopting a carbodiimide method. The prepared thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod can be applied to the isolation analysis of a trace amount of thrombin contained in complex samples, such as human serum, human plasma, human blood, and the like. The method disclosed by the invention can be used for preparing a solid-phase microextraction base body coating and controlling the thickness of the solid-phase microextraction base body coating by taking the stainless steel wire as the substrate and adopting an electrospining technology; the solid-phase microextraction base body coating is uniform, loose, porous and large in surface area; and the prepared solid-phase microextraction rod has the advantages of high specificity and high stability.

The invention discloses a thrombin detection method based on magnetic separation of deoxyribozyme and cyclic cleavage and a thrombin kit, and the thrombin detection method comprises the following steps: adding magnetic beads into a coupling buffer solution, uniformly mixing, centrifugally separating the supernatant, washing, adding a coupling buffer solution and a DNA stock solution of thrombin modified with biotin, centrifugally separating a supernatant, and washing with an incubation buffer solution for 2-3 times to obtain a magnetic bead dispersion liquid, wherein the magnetic bead concentration in the magnetic bead dispersion liquid is 4-6mg/mL; uniformly mixing the magnetic bead dispersion liquid, thrombin, a thrombin aptamer modified with deoxyribozyme and an incubation buffer solution, separating a supernatant, washing the supernatant, and dissolving the obtained solid in an enzyme digestion buffer solution to obtain a mixed solution; and adding the mixed solution into an enzyme digestion buffer solution containing a substrate chain, oscillating and incubating for 2.5-3.5 hours, carrying out magnetic separation, taking supernate, carrying out fluorescence detection, and judging the content of thrombin according to the detected fluorescence intensity. The anti-interference capability is strong, and the sensitivity is high.

The invention discloses a thrombin detection method and a kit thereof. The method comprises the following steps: 1) synthesizing a complementary PNA chain according to a DNA sequence of a thrombin aptamer; 2) mixing DNA in the thrombin aptamer with the synthesized PNA chain in the step 1) to be prepared into a substrate solution; 3) adding a color developing agent into the substrate solution and observing solution color; 4) adding a sample to be tested, observing solution color and judging a concentration of thrombin in the sample to be tested. The detection method disclosed by the invention has the advantages of quickness and convenience in detection, extremely-low requirement for instruments and equipment, high sensitivity and good specificity.

The invention belongs to the technical field of biosensors, discloses a photoelectrochemical aptamer sensor based on a sensing separation strategy and a preparation method and application of the photoelectrochemical aptamer sensor, and particularly discloses a high-performance photoelectrochemical aptamer sensor constructed based on a three-dimensional cross-linked heterojunction structure and thesensing separation strategy. The thrombin aptamer is fixed at the sensing cathode to specifically capture thrombin, so that the impedance of a sensing interface is changed, and the change of a photocurrent detection signal is caused. Based on the excellent photoelectric property of the photo-anode and the specific recognition of the sensing cathode, the sensor has high sensitivity and good selectivity on thrombin detection, an efficient preparation strategy is provided for improving the photoelectric property of the photo-anode, the detection sensitivity of the sensor on related disease markers can be effectively improved, and the method is suitable for market popularization and application.

The invention discloses a method for detecting thrombin in an ultra-sensitive mode by utilizing a competitive type nano sensor. The method is characterized in that BPE and a gold nanoparticle are combined to form gold dimer which reacts with an aptamer-1, which in complementary pairing with TBA, to obtain a Raman signal probe; the Raman signal probe performs mixing reaction with a magnetic nanoparticle modified with the thrombin aptamer TBA to obtain a nano sensor system; when to-be-detected thrombin exists, the structure of the nano sensor system is broken by competition, so that changes of aRaman signal of liquid supernatant in the system are caused, and detection for content of thrombin is realized. The method is simple to operate, is good in detecting stability and is high in detecting sensitivity; while operates, the nano sensor system which is prepared in advance is sequentially added into a to-be-detected sample to react, and detection can be immediately performed through magnetic separation after reaction is ended, so that quick and quantitative detection is realized; and requirements of food safety and environmental monitoring department can be met, and practicability iswide.

The invention relates to the formulation, dosing, administration and use of an aptamer antagonist therapeutic that binds to thrombin.

The invention relates to a bio-electrochemical sensor for detecting thrombin as well as a preparation method and application of the bio-electrochemical sensor. The sensor is a three-electrode system sensor, wherein a counter electrode is a platinum electrode, a reference electrode is a saturated calomel electrode and a working electrode is a functionally-modified pyrolytic graphite electrode; the functionally-modified pyrolytic graphite electrode is obtained by sequentially modifying poly(diallyldimethylammonium chloride), gold nanoparticles, cucurbit(7)uril and a circulating mixed solution on the surface of a pyrolytic graphite electrode; the circulating mixed solution contains 100nM of MB marked probe DNA chain, 1nM of extended DNA chain, 1nM of thrombin aptamer chain, 1.6 unit/microliter of T4DNA ligase and 0.4 unit/microliter of ExoIII. According to the bio-electrochemical sensor, specific combining capacity of the thrombin and aptamer is utilized, ExoIII circulating cutting reaction is triggered by a ligase auxiliary effect and the rapid and sensitive detection advantages of electrochemistry are combined, so that the high-sensitivity detection of the thrombin is realized.

The invention relates to a pH-responsive intelligent anticoagulant nucleic acid aptamer, the anticoagulant nucleic acid aptamer is an HD1-HD22 bivalent aptamer, an HD1 aptamer or a derivative thereof is connected with an HD22 aptamer or a derivative thereof through a bridge arm, and the bridge arm is a sequence with a pH-responsive characteristic; the sequence of the bridge arm is 5 '-TTCCTATCCGTTCCGATCCG-3' or 5 '-TTCCTATCCGTTCCAATCCG-3', and the sequence of the bridge arm is 5 '-TTCCTATCCGTTCCAATCCG-3'; the bridge arm is rich in a nucleic acid sequence of a C basic group, has the characteristic of folding and shortening the length under an acidic condition, and does not form basic group complementation with G basic groups in HD1 and HD22 sequences or destroy the structure and function of a single-chain aptamer, and the thrombin aptamers HD1 and HD22 of the bridge arm are connected to obtain the pH-responsive intelligent anticoagulant bivalent aptamer. The bivalent aptamer not only has the excellent characteristics of a common bivalent aptamer, but also can solve the problem of massive hemorrhage caused by the fact that a cancer focus compresses a weak part of a blood vessel or continuous hemorrhage of the part where the cancer focus compresses the blood vessel due to thrombocytopenia while anti-cancer thrombus treatment is carried out.

The invention belongs to the technical field of analysis and detection, and relates to a photoisomerization-based biosensor and application thereof in thrombin detection. The biosensor is a fluorescent aptamer sensor, and the fluorescent aptamer sensor is formed by taking a stilbene compound as a fluorescent signal molecule to react with a thrombin aptamer in a covalent binding manner. The aptamer sensor is combined with thrombin, thrombin detection can be achieved, and the thrombin detection capacity of the aptamer is further confirmed by changing the sequence and modification sites of the aptamer; and the preparation operation is simple, interference of background fluorescence can be avoided theoretically, complex separation steps are not needed, analysis and detection of a sample can be completed within a short time, cyclic regeneration of the aptamer sensor can be achieved, the invention provides new inspiration for developing the design of biosensors for detecting biomacromolecules such as enzyme in the future, and has important academic and application prospects.

The invention provides a preparation method and application of a thrombin-responsive DNA nano-machine. The nano-machine comprises a DNA nano-structure, a thrombolytic drug (tPA) compound and a thrombin aptamer chain structure, a tPA molecular drug is accurately loaded through arranged DNA capture chains, a controllable DNA molecular lock catch chain is utilized, and a third thrombin aptamer chain is added, so that the DNA nanostructure can be locked, and the tPA drug is entrapped in the DNA nanostructure. The DNA nano-machine disclosed by the invention can be used as a nano-scale molecular machine for loading tPA thrombolytic drugs, has high thrombin responsiveness, and can effectively respond to a thrombin environment at a thrombus part and accurately release thrombin, so that thrombolytic treatment is effectively transported to the thrombus part, and the thrombolytic effect is improved. The novel thrombolysis machine is accurate in drug loading, controllable in release and high in thrombolysis effect.