47 results about "Molecular machine" patented technology

The invention discloses a lead ion detection kit based on constant-temperature cascade nucleic acid amplification and a detection method of the lead ion detection kit. Lead ions are recognized through deoxyribozyme, strand displacement amplification and a single chain triggered DNA (deoxyribonucleic acid) molecular machine are combined to realize constant-temperature cascade nucleic acid amplification, and the concentration of the lead ions is converted into a remarkably-amplified fluorescence detection signal, thus the concentration of trace lead ions can be precisely measured. Compared with other methods based on deoxyribozyme, the detection method is rapider and higher in sensitivity; and in addition, the requirement for equipment is lower, and the operation is simpler.

The invention discloses a tagatose preparation method and belongs to the field of multi-enzyme catalytic preparation of tagatose. Starch, cellulose, or their derivatives, or sucrose is used as substrate, and the substrate is efficiently catalyzed in a multi-enzyme reaction system by an in-vitro multi-enzyme molecular machine into tagatose. By adding enzymes capable of accelerating hydrolysis of starch, cellulose or sucrose and enzymes making use of the byproduct glucose, material conversion rate and tagatose yield are significantly increased. The method of the invention is high in tagatose yield, the materials are cheap, the production cost is low, and the method is suitable for industrial production of tagatose.

The present invention discloses a method for assembling and preparing novel organic solid fluorescent material by cucurbituril anions, and belongs to the field of organic luminous materials. According to the method, macrocycle host compound cucurbituril is introduced into a specific organic fluorescent compound solution system; and by virtue of unique molecular recognition and assembly properties of the cucurbituril, the macrocycle host compound cucurbituril and a fluorescent functional compound form an anion assembly. The method can change a deposition mode of fluorescent molecules and perform effective controllable adjustment on fluorescent wavelength of the original fluorescence organic by the anion assembly, and even organic compounds that do not have the fluorescence property originally have the fluorescence property after being assembled with the cucurbituril anion. The method is simple in preparation method, and the obtained solid fluorescent material is high in yield rate and has the advantage of being capable of performing designing and modulating on a target fluorescence emission wavelength; and the method has a potential application value in various field such as color displays, anti-counterfeit marks, fluorescence sensors, molecular machines and the like.

A method is provided for exfoliation of carbon nanotubes and for the preparation of a stable aqueous suspension thereof containing dispersed, essentially single tubes, using a water-soluble polymeric material. A powder of carbon nanotubes is further provided, that can be re-dispersed to form a stable suspension. The nanotubes can be used in electronics, printing, coatings, thin layers, molecular machines, and for the production of composite materials.

The invention belongs to the biotechnological field and particularly relates to a method for detecting miRNA. The method comprises the steps that S1, a DNA molecular machine for specific recognition of miRNA is provided, the DNA molecular machine contains a nucleotide sequence of complementary sequences of a G-four-stranded-body sequence; S2, the DNA molecular machine and a sample to be detected are mutually recognized; S3, DNA polymerase and nickase are introduced to act on the DNA molecular machinery; S4, thioflavin T is introduced to induce production of the G-four-stranded-body structure and combination with a G-four-stranded-body; S5, the fluorescence radiation situation is recorded. The DNA molecule generating machine is a nucleotide sequence consisting of zones arranged in sequencefrom 3' to 5', namely a miRNA sequence recognition zone, a stable zone, nickase recognition zone and a G-four-stranded-body synthesis template. The method does not need fluorescent probe labeling, hashigher sensitivity and is simpler in operation.

The invention provides perovskite-type lead-halide-based organic-inorganic hybridized molecular machines containing crown ether and a production method and application thereof. The molecular machineshave a chemical general formula of (ABPbX2Y)n, wherein A is monovalent cation, B is crown ether, X and Y refer to halogen anions, n represents the number of repeat units and is equal to mNA, with m>0,and NA is Avogadro's constant. The crown ether is introduced to complex with the monovalent cation, the halogen anions for balancing the monovalent cation may coordinate with lead halide, such that inorganic component [Pb(X2Y)-]n and organic component [(AB)+]n are connected and assembled via Y-A bond; as atomic positions of C and O in the crown ether change constantly, the disordered structure enables [(AB)+]n to constantly rotate around [Pb(X2Y)-]n, and a series of perovskite-type lead-halide-based organic-inorganic hybridized molecular machines are obtained. The molecular machines may alsoact as dielectric materials, light-emitting materials, ferroelectric materials and/or photocatalytic materials.

The invention discloses a rotaxane molecular machine of naphthalimide crown ether and a pH meter based on the molecular machine. Structural formula of the molecular machine is represented as a formula (I) as shown in the specification. The pH meter includes a gold electrode and the rotaxane molecular machine of the naphthalimide crown ether which is adsorbed on the surface of the gold electrode to form a self-assembled monolayer. The synthesized rotaxane molecular machine of the naphthalimide crown ether is bonded with the gold electrode through a disulfide bond, to result in transfer of the molecular machine and charges on the gold electrode in a process of adding acid or alkali, thus achieving online rapid monitoring of pH value; the molecular machine can avoid accumulation of waste generated from the whole monitoring process on the monolayer, thus enhancing detection limit, sensitivity and fatigue resistance.

The invention relates to the medical field of immunoassay, and specifically, the invention innovatively designs a prostate-specific antigen detection kit based on second antibody modified shear enzyme initiated DNA molecular machine cycle amplification. The kit provided by the invention includes: an anti-PSA antibody coated solid phase carrier, a shear enzyme labeled anti-fPSA antibody, a reaction solution (DNA molecular machine) on which the shear enzyme acts, and a negative control solution and a positive control solution. The kit provided by the invention has high sensitivity, and compared with the existing optical immunoassay detection method, can improve the analysis sensitivity and reproducibility, and reduce the antibody dosage.

The invention provides a DNA molecular machine and also provides a single-base mutation detection method based on the DNA molecular machine. The method comprises the following steps: initiating different running capabilities of the DNA molecular machine by utilizing different catalytic chains, combining assembly of gold nanoparticles, observing the reaction difference of a catalytic chain system in which completely complementary catalytic chains are added and the catalytic chain system with single-base mutation through a contrast experiment, and finally realizing the single-base mutation detection based on the DNA molecular machine. Strict operating conditions are not needed in the detection means, and the operation is simple. The detection means of the signal is simple and can be identified through colors and ultraviolet spectrums in actual application. The difficulty that complex operation exists or a precise instrument needs to be used for observation in a conventional detection technology is solved. The detected base mutation type is comprehensive and comprises all base mutation forms, namely base mismatch, base deletion and base insertion, and a mutation position of the detected DNA chain is random.

The invention provides a DNA molecular machine for detecting exosome, which is formed by combining a characteristic Substate chain and an Aptamer locked Motor chain on the surface of gold nanoparticles (GNP), and is used for fluorescence detection of a specific biomarker in the exosome so as to determine the concentration of the exosome. According to the invention, CD63 protein on an exosome membrane and CD63 aptamer for locking a Motor chain on a DNA molecular machine are used for specific recognition, thus unlocking a Motor chain, combining the Motor chain with a Substate chain, and cuttingoff the Substate chain by the restriction endonuclease, so that the DNA molecular machine begins to operate and releases fluorescent molecules. The DNA molecular machine provided by the invention hashigh specificity and sensitivity and is low in cost; and the CD63 aptamer can also be replaced with aptamers of other specific biomarkers, so that the detection range of a DNA molecular machine is expanded.

The invention relates to a fluorescent molecular machine based on one-dimensional silicon nanowires and a using method thereof. The fluorescent molecular machine based on one-dimensional silicon nanowires consists of one-dimensional nanowires modified by 3-aminopropyltriethoxysilane, as well as N,N-bis-(4-p-formylphenylazobenzene-4'-methyl)-dansylamide and alpha-cyclodextrin, wherein the alpha-cyclodextrin is sleeved on the N,N-bis-(4-p-formylphenylazobenzene-4'-methyl)-dansylamide, and the N,N-bis-(4-p-formylphenylazobenzene-4'-methyl)-dansylamide is bonded with the one-dimensional nanowires modified by the 3-aminopropyltriethoxysilane by covalent bonds. In the invention, ultraviolet light and visible light are used as input to control the motion of the fluorescent molecular machine and fluorescence is used as output to demonstrate the motion of the fluorescent molecular machine.

The invention relates to a method for rapidly detecting fusarium DNA. The method comprises the following steps: (S1) providing a DNA molecular machine for specifically recognizing fusarium DNA, wherein the DNA molecular machine is a nucleotide sequence containing a G-quadruplex complementary sequence; (S2) mutually mixing the DNA molecular machine with a to-be-tested sample; (S3) introducing DNA polymerase and nickase for the DNA molecular machine so as to generate DNA amplification reaction; and (S4) introducing thioflavine T for inducing and generating a G-quadruplex structure, and binding with the G-quadruplex structure to generate fluorescence; and detecting and recording the fluorescence. According to the method, a nucleic acid signal in the to-be-tested sample suspected to contain fusarium DNA is converted into G-quadruplex, and the continuous amplification of G-quadruplex can be realized at the constant room temperature, so that the DNA signal of the nucleic acid is greatly amplified; and a stable fluorescence signal is generated through the binding of thioflavine T and G-quadruplex, so that the high-sensitivity, rapid and qualitative analysis assay determination of nucleicacid is realized.

Carriers for biomaterials, for polyelectrolyte materials, for electrically polarizable atoms, ions, molecules are provided wherein the material of the carriers is compatible with materials that are used in microelectronics. The arrangement of the biomaterials or biomolecules and optionally of biomolecules, biomaterials, biological functional units or cells adsorbed thereon can be affected with the carrier in a specific manner. Complex molecular machines can be built and tested by the carriers.

Disclosed herein are embodiments of nanohoop compounds, methods of making, and methods of using the same. The nanohoop compounds disclosed herein have discrete ring system(s) that comprise a unique meta-substituted motif that affords a strained cavity in which myriad reaction chemistries can take place. The unique structures and properties of the nanohoop compounds disclosed herein also lend to their use in a variety of biological applications, and as interlocked structures in molecular machines.

The invention discloses a method for preparing inositol-1-phosphate, which belongs to the field of enzyme catalysis preparation of inositol-1-phosphate. The preparation method of the inositol-1-phosphate disclosed by the invention uses starch and its derivative and phosphate as substrates to efficiently convert the substrate into inositol-1-phosphate by in vitro multi-enzyme molecular machine catalysis. The method adds the enzyme capable of promoting hydrolysis of starch and its derivative and the enzyme utilizing by-product glucose to obviously increase the yield of the inositol-1-phosphate.The method of the invention has high yield of inositol-1-phosphate, simple production equipment, and low production cost, and can realize large-scale prepration of inositol-1-phosphate.

The invention discloses a drug-resistant bacterium detection method based on nucleic acid molecular machine colorimetry. The method comprises the following steps: (1) constructing double probes with bacterial specific response: mixing sulfydryl-modified double probe chains 1 and 2 with a nano material, adjusting the pH value, and then performing standing and centrifuging to obtain double Probe1 and Probe2 with bacterial specific response; (2) preparing a reaction solution: storing the double probes with the bacterial specific response and a reaction mixture into a centrifugal tube to prepare the reaction solution, wherein the reaction mixture comprises a double-stranded nucleic acid (RNA1-RNA2-Aptamer) and EXOIII; and (3) detecting bacteria: mixing a bacteria solution to be detected with the reaction solution prepared in the step (2) according to a certain volume ratio, carrying out incubation reaction, measuring ultraviolet absorptivity, and determining bacteria types. According to the method, signal cyclic amplification is carried out at a double enzyme digestion rate, common drug-resistant bacteria in hospitals can be identified more quickly, the whole detection process is shorter than 4 hours, and sensitivity and accuracy are achieved; and the method has the advantages of being good in specificity, simple to operate and visible to the naked eyes, and lays a foundation for research and development of rapid bacterial diagnosis kits.