6 results about "Molecular machine" patented technology

The invention relates to the field of veterinary drug residue detection, in particular to a fluorescence sensor based on DNA molecular machine mediated split Cas12a and application of the fluorescence sensor to ofloxacin detection, the fluorescence sensor comprises a system A, a system B and a system C. According to the final concentration of each system, the system A comprises an AP / CP double-stranded compound and a 20 mM Tris-HCl buffer solution 1, the system B comprises a double-stranded compound, an sRNA probe and a 10 mM Tris-HCl buffer solution 2, and the system C comprises a DNA molecular machine mediated split Cas12a. The system C comprises a Cas12a / hRNA compound, a pDNA / psG43 signal probe, ThT and a 20 mM Tris-HCl buffer solution; aP in the AP / CP double-chain compound is an aptamer of ofloxacin, and AP and CP in the AP / CP double-chain compound are paired and connected through complementary base pairing; the sequences of AP, CP, PS, SS, sRNA, hRNA, pDNA and psG43 are respectively as shown in SEQ ID NO.1-SEQ ID NO.8. The invention also discloses a kit for detecting the content of the protein. According to the application, the nucleic acid aptamer of ofloxacin is utilized, based on a DNA molecular machine and SCas12a as a signal amplification technology and sulfo-modified G43 as a fluorescent signal probe, even if the content of ofloxacin in a sample is extremely low, detection can be accurately realized.

The application provides a protein molecular machine motion mode simulation method, comprising the following steps: S10, obtaining a protein molecular machine structure data file; S20, simulating the molecular dynamics of the protein molecular machine to obtain a complex and its receptor, ligand motion topological file and coordinate file, and calculating the binding free energy thereof; S30, predicting the motion trajectory of the protein molecular machine based on the built NRI model and the coordinate file, wherein the NRI model comprises an encoder for predicting the interaction of a given dynamic system trajectory, and a decoder for predicting the given dynamic system trajectory in the interaction graph; and S40, observing and recording the three-dimensional structure and motion trajectory of the protein molecular machine based on a VR device. The application realizes accurate simulation and prediction of the motion mode of the protein molecular machine by combining molecular dynamics simulation and deep neural network.

The application provides an engineered DNAzyme molecular machine and its application, including one or two groups of chain group one and chain group two, chain group one: i-motif chain modified DNAzyme and its substrate chain, the chain group one is modified on the surface of cancer cells and T cells, the acid microenvironment of tumor cells is used to make the modified i-motif chain contract, and the distance between the cancer cells and the tumor cells is shortened; chain group two: mitochondria-targeting peptide modified DNAzyme and its substrate chain, intracellular mitochondrial aggregation is used to induce apoptosis. The tumor microenvironment responsive control of cell behavior outside and inside cells based on DNAzyme includes cell recognition, intercellular proximity, separation after treatment and mitochondrial aggregation, which not only provides a useful way for regulating intercellular interaction, but also provides a powerful targeted cancer treatment method without killing normal cells.

This invention discloses a light-driven molecular machine and its application in transmembrane delivery of trivalent metal drugs. The molecular machine features a light-driven molecular motor based on a tetrasubstituted crowded olefin, which consists of a core unit, a polyethylene glycol chain, and trivalent metal ion coordinating groups. This invention utilizes the directional rotational motion of the molecular motor under light irradiation, embedding it into a phospholipid bilayer membrane, and achieving light-controlled transmembrane transport of trivalent metal ions through a "bind-release" mechanism. This molecular machine can selectively complex trivalent metal ions (such as Bi...) 3+ Ga 3+ Ce 3+ (etc.), and accelerates ion transmembrane transport under light-driven conditions, thereby disrupting the intracellular and extracellular ion balance of bacteria. Experiments have shown that this system has significant light-controlled antibacterial activity against Staphylococcus aureus. This invention has advantages such as controllable structure, precise spatiotemporal targeting, and ease of derivatization, and has promising applications in antibacterial therapy and biomedicine.

The application belongs to the technical field of molecular machines, and particularly relates to a fluorine-containing rotaxane molecule and a preparation method and application thereof. The fluorine-containing rotaxane molecule of the application takes fluorine-containing crown ether as a cyclic molecule, and is also a fluorine-19 magnetic resonance signal source; takes a chain molecule containing a secondary ammonium salt cation as an axle, and can control the relaxation property of fluorine through the interaction with the fluorine-containing crown ether; and takes 3,5-substituted benzene derivatives as end-capping groups to prevent the fluorine-containing crown ether from falling off from the axle. The molecule can be used as a fluorine-19 magnetic resonance contrast agent, and based on the structure characteristics of the rotaxane, the motion of the molecule is limited to improve the sensitivity of magnetic resonance imaging, a paramagnetic metal is not needed, the fluorine signal is single and strong, and the structure is convenient for subsequent functionalization, so that a high-performance 19 F MRI contrast agent is provided.

The invention discloses a porphyrinyl molecular machine and an application thereof, and relates to a design and construction method of a stator-rotor-regulatory factor trinity porphyrinyl molecular machine and an antibacterial application of the porphyrinyl molecular machine. The porphyrin-based molecular machine is anchored on the surface of bacteria through electrostatic interaction, and a bacterial biofilm is creatively destroyed by creatively utilizing the mechanical rotation motion of the porphyrin-based molecular machine on the molecular scale, so that the antibacterial purpose is achieved. Safe and effective antibiosis can be realized, and drug resistance of bacteria can be avoided.