21 results about "Molecular machine" patented technology

The invention provides a fluorescent / photo-thermal dual-mode sensor constructed based on an entropy-driven DNA molecular machine as well as a construction method and application of the fluorescent / photo-thermal dual-mode sensor, and belongs to the technical field of sensor construction and analytical testing. The fluorescent / photo-thermal dual-mode sensor is constructed on the basis of Ag (at) Au NPRs with good photo-thermal performance, CdTe QDs with strong fluorescence signals, a Fe3O4 (at) SiO2 nano material with superparamagnetism and an entropy-driven DNA molecular machine signal amplification strategy capable of being specifically combined with miRNA; the fluorescence detection range of the fluorescence / photo-thermal dual-mode sensor is 1 fM to 50 pM, the detection limit is 0.34 fM, the photo-thermal detection range is 1 pM to 1 nM, and the detection limit is 0.5 pM; the fluorescent / photo-thermal dual-mode sensor is simple in construction method, convenient and rapid in detection and high in selectivity, has very good practicability and has very good application in miRNA detection.

The invention relates to the field of veterinary drug residue detection, in particular to a fluorescence sensor based on DNA molecular machine mediated split Cas12a and application of the fluorescence sensor to ofloxacin detection, the fluorescence sensor comprises a system A, a system B and a system C. According to the final concentration of each system, the system A comprises an AP / CP double-stranded compound and a 20 mM Tris-HCl buffer solution 1, the system B comprises a double-stranded compound, an sRNA probe and a 10 mM Tris-HCl buffer solution 2, and the system C comprises a DNA molecular machine mediated split Cas12a. The system C comprises a Cas12a / hRNA compound, a pDNA / psG43 signal probe, ThT and a 20 mM Tris-HCl buffer solution; aP in the AP / CP double-chain compound is an aptamer of ofloxacin, and AP and CP in the AP / CP double-chain compound are paired and connected through complementary base pairing; the sequences of AP, CP, PS, SS, sRNA, hRNA, pDNA and psG43 are respectively as shown in SEQ ID NO.1-SEQ ID NO.8. The invention also discloses a kit for detecting the content of the protein. According to the application, the nucleic acid aptamer of ofloxacin is utilized, based on a DNA molecular machine and SCas12a as a signal amplification technology and sulfo-modified G43 as a fluorescent signal probe, even if the content of ofloxacin in a sample is extremely low, detection can be accurately realized.

The invention discloses a porphyrinyl molecular machine electrochemical luminescence biosensor. The porphyrinyl molecular rotor is coupled with a potassium ion aptamer, and the effect of a biosensor is achieved by utilizing the relevance between the spin characteristic of the porphyrinyl molecular rotor and electrochemical luminescence. According to the invention, the efficiency and sensitivity of the biosensor are improved, and an efficient and sensitive detection means is provided for the field of biosensing.

To provide a molecular structure which allows seamless communication and cooperation between functional elements to perform multiple programmable tasks at once.SOLUTION: A programmable molecular structure (100) comprises a matrix molecule (110), one or more receptor molecules (103, 104, 105), at least one of which is linked to a portion of the matrix molecule (110), one or more molecular machines (106, 107), at least one of which is linked to another portion of the matrix molecule (110), and one or more controller molecules (101) that control at least a portion of the molecular machines (106, 107).SELECTED DRAWING: Figure 1

This invention discloses a photosynthetic nanomotor powered by a biomolecular motor and its preparation method, belonging to the field of colloidal motors. This invention aims to address the current limitations of research on the rotational dynamics of FoF1-ATP synthase molecular machines, which is limited to the motion of single and isolated objects. The photosynthetic nanomotor is prepared based on supramolecular self-assembly technology through the reconstruction of thylakoid vesicles and lecithin vesicles. The photophosphorylation system on the thylakoid membrane surface is reconstructed on the photosynthetic nanomotor. Utilizing the preserved biomolecular motor FoF1-ATPase as the power engine, under external visible light irradiation, protons generated by the photosystem accumulate in the photosynthetic liposome motor, driving the FoF1-ATPase to synthesize the energy currency ATP. Through metabolic reactions, light energy is converted into chemical energy ATP, ultimately generating self-propulsion. Due to its unique energy currency regeneration capability, the photosynthetic liposome motor has broad application prospects in the biomedical field.

The invention discloses a molecular machine based on programmable DNA nanotube mediation and a detection method of exosome nucleic acid of the molecular machine, and the preparation method of the molecular machine based on programmable DNA nanotube mediation is characterized by comprising the following steps: (1) complementarily pairing target exosome nucleic acid miR-193b and a template sequence Probe A to obtain a product extension chain; (2) carrying out catalytic extension and cutting on an extension chain by using Klenow polymerase, i.e., carrying out multiple rounds of polymerization and cutting on the product in the step a to generate a large amount of Probe B for subsequent reaction; and (3) enabling S1, S2, S3-DNAzyme, S4 and S5 to react in a TM buffer solution at 37 DEG C for 1 hour, so as to prepare the blocked molecular machine based on the programmable DNA nanotube mediation. The technology realizes high-sensitivity and high-selectivity nucleic acid detection, and has important clinical diagnostic value.

The present invention discloses an estradiol biosensor and its construction method and application, belonging to the field of biosensor technology. The estradiol biosensor of the present invention comprises a sensing substrate and a DNA molecular machine; the sensing substrate is a gold electrode modified with an IP-BP double chain; the DNA molecular machine comprises an Aptamer-TP double chain and an FP-N3 probe. The biosensor of the present invention uses an estradiol aptamer probe to specifically identify estradiol, constructs a DNA molecular machine for signal cyclic amplification, and uses an atom transfer radical polymerization (ATRP) reaction to perform polymerization growth of electrical signals, thereby achieving efficient and amplified detection of estradiol, improving detection accuracy and sensitivity, and can be applied to the accurate detection of E2 content in clinical blood samples. It has important application value in research fields such as predicting pregnancy outcomes, endocrine diseases, and precocious puberty.

The application provides a protein molecular machine motion mode simulation method, comprising the following steps: S10, obtaining a protein molecular machine structure data file; S20, simulating the molecular dynamics of the protein molecular machine to obtain a complex and its receptor, ligand motion topological file and coordinate file, and calculating the binding free energy thereof; S30, predicting the motion trajectory of the protein molecular machine based on the built NRI model and the coordinate file, wherein the NRI model comprises an encoder for predicting the interaction of a given dynamic system trajectory, and a decoder for predicting the given dynamic system trajectory in the interaction graph; and S40, observing and recording the three-dimensional structure and motion trajectory of the protein molecular machine based on a VR device. The application realizes accurate simulation and prediction of the motion mode of the protein molecular machine by combining molecular dynamics simulation and deep neural network.

The invention relates to the field of molecular machines, in particular to a signal system for continuous autonomous movement of a derailment-free three-drive DNA harvester along a nanogold solidified nucleic acid substrate track, a preparation method and application. The signal system comprises a three-drive DNA harvester and a nanogold curing substrate track; the three-drive DNA harvester comprises a DNA tetrahedron, a nucleic acid aptamer used for recognizing target cells is assembled at one fixed point vertex of the DNA tetrahedron, and locking foot chains are assembled at the other three vertexes of the DNA tetrahedron; the nanogold curing and nucleic acid substrate orbital is formed by covalent binding of three linear sequences and nanogold particles and complementary pairing of part of basic groups; the three linear sequences are two split enzyme chains and a substrate chain, and the substrate chain modifies a fluorophore and has a deoxyribozyme digestion site. The signal system provided by the invention has the advantages of no cytotoxicity, nuclease degradation resistance, three-drive autonomous continuous movement, avoidance of orbital detachment and sequential precise recognition mediated by double biomarkers.

A framework consistent with the thermodynamic principle and used for deciphering a DNA methylation process utilizes probability density functions of DNA methylation information divergence to summarize statistical biophysics forming the basis of a spontaneous methylation background, and is related to the channel capacity of a molecular machine conforming to the Shannon's capacity theorem (Shannon's capacity theorem). The contribution of a molecular machine (enzyme) logic operation to a Gibbs entropy (Gibbs entropy) (S) and a Helmholtz free energy (Helmholtz free energy) (F) is intrinsic. Application in the biomedicine and biopharmaceutical industries can be achieved by estimating S on a methylation group data set. As a thermodynamic state variable, a single methylation group entropy is determined entirely from the current state of the system, biologically translated into a correspondence between an estimated entropy value and an observable phenotypic state. Analysis of entropy fluctuations in experimental data sets shows that there is a limit in the magnitude of whole genome methylation changes during the response of organisms to environmental changes, thereby allowing early diagnosis and prediction of epigenetic state changes.

A framework consistent with thermodynamic principles to decipher the DNA methylation process utilizes a probability density function of DNA methylation information-divergence, summarizes the statistical biophysics underlying spontaneous methylation background, and bears on the channel capacity of molecular machines conforming to Shannon's capacity theorem. Contributions from the molecular machine (enzyme) logical operations to Gibbs entropy (S) and Helmholtz free energy (F) are intrinsic. Biomedical and biopharmaceutical industrial applications are achievable by way of estimating S on methylome datasets. As a thermodynamic state variable, the individual methylome entropy is completely determined by the current state of the system, which in biological terms translates to a correspondence between estimated entropy values and observable phenotypic state. Analysis of entropy fluctuations on experimental datasets revealed the existence of restrictions on the magnitude of genome-wide methylation changes during organismal response to environmental changes, thereby allowing for earlier-stage diagnostics and prediction of epigenetic state changes.

The DNA molecular machine makes a major breakthrough in the fields of biosensing, bioimaging, early cancer diagnosis and the like. However, the current DNA molecular machine strategy is continuously challenged by insufficient analysis sensitivity, and a precise signal amplification program is often essential. Single-particle inductively coupled plasma mass spectrometry (SP-ICPMS) has excellent sensitivity, and background noise and detection signals can be effectively distinguished. In the research, a novel method is provided, and a 3D DNA molecular machine is constructed by using single nanoparticle counting and bispecific nuclease (DSN) assisted signal amplification and is used for detecting the invasive prostate cancer (PCa) biomarker miRNA-200c. The strategy is improved in sensitivity, the limit of detection (LOD) is 0.93 pM (28 amol), and the strategy is successfully applied to human serum sample analysis.

The invention relates to a method for preparing L-glufosinate-ammonium by splitting racemic glufosinate-ammonium (D, L-glufosinate-ammonium) through an electrochemical multi-enzyme molecular machine, which comprises the following steps: catalyzing D-glufosinate-ammonium by D-amino acid oxidase to obtain 2-carbonyl-4-[hydroxy (methyl) phosphonyl] butyric acid, and catalyzing by amino acid dehydrogenase to obtain L-glufosinate-ammonium. And an electrochemical multi-enzyme molecular machine is utilized to realize dynamic monitoring of the preparation process. By detecting the current change, the reaction kinetics and process are known, and the enzyme catalysis efficiency is improved; nADP < + > green efficient cyclic regeneration is achieved, separation of an intermediate product is not needed, L-glufosinate-ammonium is directly obtained, the conversion efficiency reaches up to 90% or above, and the enantiomer excess rate is gt; 99%; the method is simple to operate, green, efficient, high in selectivity and suitable for large-scale popularization and demonstration of L-glufosinate-ammonium.

The present invention discloses a DNA assembly molecular machine and its applications, relating to the field of molecular biotechnology. The DNA assembly molecular machine comprises a palm domain, two terminal insertion domains, a thumb domain, a finger domain, and an exonuclease activity domain. The DNA assembly molecule exhibits excellent stability and is suitable for isothermal amplification. Furthermore, the DNA assembly molecule and its mutants possess DNA assembly capabilities in the presence of various metal ions and at different temperatures, and can be applied to nanopore sequencing, single-molecule sequencing, RCA library construction, environmental testing, and in vitro diagnostics.

The application provides an engineered DNAzyme molecular machine and its application, including one or two groups of chain group one and chain group two, chain group one: i-motif chain modified DNAzyme and its substrate chain, the chain group one is modified on the surface of cancer cells and T cells, the acid microenvironment of tumor cells is used to make the modified i-motif chain contract, and the distance between the cancer cells and the tumor cells is shortened; chain group two: mitochondria-targeting peptide modified DNAzyme and its substrate chain, intracellular mitochondrial aggregation is used to induce apoptosis. The tumor microenvironment responsive control of cell behavior outside and inside cells based on DNAzyme includes cell recognition, intercellular proximity, separation after treatment and mitochondrial aggregation, which not only provides a useful way for regulating intercellular interaction, but also provides a powerful targeted cancer treatment method without killing normal cells.

The invention discloses an estradiol biosensor as well as a construction method and application thereof, and belongs to the technical field of biosensors. The estradiol biosensor comprises a sensing substrate and a DNA molecular machine, the sensing substrate is a gold electrode modified with IP-BP double chains; and the DNA molecular machine comprises an Aptamer-TP double strand and an FP-N3 probe. According to the biosensor, the estradiol aptamer probe is adopted for specific recognition of estradiol, a DNA molecular machine is constructed for signal cycle amplification, polymerization growth of an electric signal is carried out by means of an atom transfer radical polymerization (ATRP) reaction, efficient and amplified detection of estradiol can be achieved, the detection accuracy and sensitivity are improved, and the biosensor is suitable for mass production. The method can be applied to accurate detection of the E2 content in a clinical blood sample, and has important application value in the research fields of prediction of pregnancy outcome, endocrine diseases, precocious puberty and the like.

A method may include obtaining a ribonucleic acid (RNA) sequence from a protein data bank comprising a plurality of RNA sequences. The method may also include generating a plurality of candidate RNA secondary structures by passing the RNA sequence through at least one RNA secondary structure prediction model. The method may further include assembling, via a RNA tertiary structure generator, the plurality of candidate RNA secondary structures into a plurality of RNA tertiary structures. The method may also include ranking the plurality of RNA tertiary structures by implementing a plurality of thermodynamic molecular machine learning models. The method may further include determining a structural binding affinity of small-molecule based on the ranked plurality of RNA tertiary structures.

The invention discloses a method for observing the directional motion of a molecular machine based on a microfluidic technology, which monitors and analyzes the overall migration direction of sandwich type metalloporphyrin molecules in real time through the characteristics of time-space conversion of the microfluidic technology, and reveals the influence of protonic acid induced rotation acceleration on the motion mode of the sandwich type metalloporphyrin molecules. Results show that the chemotactic movement of sandwich type metalloporphyrin molecules is quicker and more efficient due to rotation acceleration induced by protonic acid. The method is suitable for application of various sandwich-type metalloporphyrin molecules in the field of biological science, and valuable reference is provided for research in related fields.

This invention discloses a light-driven molecular machine and its application in transmembrane delivery of trivalent metal drugs. The molecular machine features a light-driven molecular motor based on a tetrasubstituted crowded olefin, which consists of a core unit, a polyethylene glycol chain, and trivalent metal ion coordinating groups. This invention utilizes the directional rotational motion of the molecular motor under light irradiation, embedding it into a phospholipid bilayer membrane, and achieving light-controlled transmembrane transport of trivalent metal ions through a "bind-release" mechanism. This molecular machine can selectively complex trivalent metal ions (such as Bi...) 3+ Ga 3+ Ce 3+ (etc.), and accelerates ion transmembrane transport under light-driven conditions, thereby disrupting the intracellular and extracellular ion balance of bacteria. Experiments have shown that this system has significant light-controlled antibacterial activity against Staphylococcus aureus. This invention has advantages such as controllable structure, precise spatiotemporal targeting, and ease of derivatization, and has promising applications in antibacterial therapy and biomedicine.

The application belongs to the technical field of molecular machines, and particularly relates to a fluorine-containing rotaxane molecule and a preparation method and application thereof. The fluorine-containing rotaxane molecule of the application takes fluorine-containing crown ether as a cyclic molecule, and is also a fluorine-19 magnetic resonance signal source; takes a chain molecule containing a secondary ammonium salt cation as an axle, and can control the relaxation property of fluorine through the interaction with the fluorine-containing crown ether; and takes 3,5-substituted benzene derivatives as end-capping groups to prevent the fluorine-containing crown ether from falling off from the axle. The molecule can be used as a fluorine-19 magnetic resonance contrast agent, and based on the structure characteristics of the rotaxane, the motion of the molecule is limited to improve the sensitivity of magnetic resonance imaging, a paramagnetic metal is not needed, the fluorine signal is single and strong, and the structure is convenient for subsequent functionalization, so that a high-performance 19 F MRI contrast agent is provided.

The invention discloses a porphyrinyl molecular machine and an application thereof, and relates to a design and construction method of a stator-rotor-regulatory factor trinity porphyrinyl molecular machine and an antibacterial application of the porphyrinyl molecular machine. The porphyrin-based molecular machine is anchored on the surface of bacteria through electrostatic interaction, and a bacterial biofilm is creatively destroyed by creatively utilizing the mechanical rotation motion of the porphyrin-based molecular machine on the molecular scale, so that the antibacterial purpose is achieved. Safe and effective antibiosis can be realized, and drug resistance of bacteria can be avoided.