Three-dimensional composite material of titanium carbide, and preparation method thereof, and application in constructing thrombin aptasensor

An aptamer sensor and composite material technology, applied in the analysis of materials, material analysis through electromagnetic means, instruments, etc., can solve the problems of cumbersome and complicated processes, achieve accelerated electron transfer rate, good electrochemical performance, and improve charge separation efficiency Effect

Active Publication Date: 2019-04-02
QINGDAO UNIV
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  • Three-dimensional composite material of titanium carbide, and preparation method thereof, and application in constructing thrombin aptasensor
  • Three-dimensional composite material of titanium carbide, and preparation method thereof, and application in constructing thrombin aptasensor
  • Three-dimensional composite material of titanium carbide, and preparation method thereof, and application in constructing thrombin aptasensor

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preparation example Construction

[0034] In a first exemplary embodiment of the present disclosure, a M NPs / TiO 2 / Ti 3 C 2 T X A method for preparing a composite material, the method comprising:

[0035] layered Ti 3 C 2 T X , noble metal salt and water are mixed evenly, then hydrothermally reacted at 160-200°C for 12-16 hours, cooled and left to stand for 12-15 hours to prepare M NPs / TiO 2 / Ti 3 C 2 T X composite material.

[0036] The present disclosure adopts a one-pot hydrothermal method to directly reduce noble metal nanoparticles (M NPs) to the composite material TiO 2 / Ti 3 C 2 T X At the same time, after the end of the hydrothermal reaction, the TiO 2 Growth of nanorods to obtain TiO 2 It is a nanorod structure and its size is relatively large. The inventor unexpectedly found that this structure is more conducive to stretching Ti 3 C 2 T X The layered structure is conducive to improving the proton transmission rate, which is more conducive to the detection of thrombin.

[0037] Afte...

Embodiment 1

[0069] MXenes-Ti 3 C 2 T X Synthesis

[0070] 3g Ti 3 AlC 2 Slowly add 30 mL of 9M HF solution at 5°C to obtain a mixed solution. The mixed solution was stirred at 5 °C for 20 min and then at 45 °C for 24 h to obtain Ti 3 C 2 T X suspension of flakes. It was then centrifuged until the pH of the suspension reached 6.5, layered Ti was obtained by sonication for 11 h 3 C 2 T X The colloidal solution was then centrifuged at 3500 rpm for 35 minutes to remove the precipitate.

[0071] TiO 2 / Ti 3 C 2 T X Synthesis of Hydrogels

[0072] 20mL (4mg / mL) Ti 3 C 2 T X The solution was dispersed in 5 mL of water and sonicated for 1.2 h to obtain a homogeneous suspension. Then, the suspension was transferred into a Teflon sealed autoclave and heated at 180 °C for 14 h. After cooling to room temperature, let it stand for 14 hours to form TiO 2 Growth of nanorods. Synthesized three-dimensional composite nanomaterial TiO 2 / Ti 3 C 2 T X Wash with water, then store in...

Embodiment 2

[0090] MXenes-Ti 3 C 2 T X Synthesis

[0091] 3g Ti 3 AlC 2 Slowly add 30 mL of 9M HF solution at 5°C to obtain a mixed solution. The mixed solution was stirred at 5 °C for 20 min and then at 45 °C for 24 h to obtain Ti 3 C 2 T X suspension of flakes. It was then centrifuged until the pH of the suspension reached 6.5, layered Ti was obtained by sonication for 11 h 3 C 2 T X The colloidal solution was then centrifuged at 3500 rpm for 35 minutes to remove the precipitate.

[0092] TiO 2 / Ti 3 C 2 T X Synthesis of Hydrogels

[0093] 20mL (4mg / mL) of Ti3 C 2 T X The solution was dispersed in 5 mL of water and sonicated for 1.2 h to obtain a homogeneous suspension. Then, the suspension was transferred into a Teflon sealed autoclave and heated at 180 °C for 14 h. After cooling to room temperature, let it stand for 14 hours to form TiO 2 Growth of nanorods. Synthesized three-dimensional composite nanomaterial TiO 2 / Ti 3 C 2 T X Wash with water, then store i...

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Abstract

The invention relates to a three-dimensional composite material of the titanium carbide, and a preparation method thereof, and an application in constructing a thrombin aptasensor. A substrate of thethree-dimensional composite material TiO2/Ti3C2TX is formed by the self-growth of TiO2 nanorods on the surface of Ti3C2TX by one-step hydrothermal method; meanwhile, the three-dimensional composite material M NPs/TiO2/Ti3C2TX is obtained by reducing noble metal nanoparticles (M NPs) on the surface of the three-dimensional material. The material has a highest current intensity, due to the fact thata variety of enhancements are achieved in one step. The three-dimensional structure of the composite material may provide a particularly large accessible surface area, which facilitates the anchoringof the M NPs. The introductions of transition metal carbonitrides (MXenes) and metal nanoparticles can improve the efficiency of the charge separation, and accelerate the electron transfer rate. A sensitive unlabeled aptamer is successfully established through the combination of M NPs and aptamer chains, and is used for determining the enzyme protein. According to the three-dimensional compositematerial of the titanium carbide, and the preparation method thereof, and the application in constructing thrombin aptasensor, the aptasensor has a good electrochemical performance, a wide linear range, and a relatively low detection limit, thereby indicating that the M NPs/TiO2/Ti3C2TX will be promising for the use as electrode materials in electrochemical biosensors.

Description

technical field [0001] The disclosure specifically relates to a titanium carbide three-dimensional composite material, a preparation method thereof, and an application in constructing a thrombin aptamer sensor. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Thrombin converts fibrinogen to fibrin. After local application, the blood acting on the lesion surface rapidly forms a stable clot, which is used to control capillary, venous bleeding, or as an adhesive and fixative for skin and tissue grafts, and is involved in various diseases such as thromboembolic Diseases, leukemia and inflammatory responses. To adapt to global health concerns, especially in developing countries, it is highly desirable to develop simple, low-cost and effective methods for the quantitative detection of thrombin. Some conventional methods for protein analysis, such as ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 张菲菲邹慧燕王宗花杨潇
Owner QINGDAO UNIV
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