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Preparation method and application of a photoelectrochemical thrombin aptasensor based on ps@au dual inhibition zncds

An aptamer sensor, photoelectrochemical technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problems of low detection sensitivity, easy to be affected by the environment, complicated operation, etc., to reduce the background signal and achieve monodisperse performance. Good, easy-to-use results

Active Publication Date: 2021-06-04
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection methods have problems such as expensive instruments, complicated operation, easy to be affected by the environment, and low detection sensitivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The specific steps for preparing ZnCdS are:

[0038] Weigh 1 g Zn(Ac) 2 2H 2 O and 1.5 g Cd(NO 3 ) 2 4H 2 O In a round bottom flask, add 20 mL of ultrapure water and 1 mL of ammonia water, then slowly drop 5 mL of ionic liquid into the round bottom flask, sonicate the above mixed solution for 30 min, and dissolve another 2 g of sodium sulfide in 8 mL Ultrapure water and sonication for 30 min, after sonication, it was added dropwise to the round bottom flask under high-speed stirring, the solution turned yellow after 1 h of reaction, and then the mixed solution was transferred to a 50 mL reaction kettle, at 100 °C Calcined for 8 h, the product was washed three times with ultrapure water and absolute ethanol, and dried in a vacuum oven at 90 °C for 10 h, and then the product was naturally cooled to room temperature to obtain a yellow zinc-cadmium co-doped sulfide ZnCdS nanocrystals are dissolved in ultrapure water to obtain a ZnCdS suspension;

[0039] The...

Embodiment 2

[0040] The specific steps of embodiment 2 preparing ZnCdS are:

[0041] Weigh 2 g Zn(Ac) 2 2H 2 O and 3 g Cd(NO 3 ) 2 4H 2 O In a round-bottomed flask, add 40 mL of ultrapure water and 2 mL of ammonia water, then slowly drop 10 mL of ionic liquid into the round-bottomed flask, ultrasonicate the above mixed solution for 40 min, and dissolve another 4 g of sodium sulfide in 16 mL Ultrapure water and sonication for 40 min, after sonication, it was added dropwise to the round bottom flask under high-speed stirring, the solution turned yellow after 1.5 h of reaction, and then the mixed solution was transferred to a 100 mL reaction kettle, at 120 °C Calcined for 8 h, the product was washed three times with ultrapure water and absolute ethanol, and dried in a vacuum oven at 90 °C for 12 h, and then the product was naturally cooled to room temperature to obtain a yellow zinc-cadmium co-doped sulfide ZnCdS nanocrystals are dissolved in ultrapure water to obtain a ZnCdS suspension;...

Embodiment 3

[0043] The specific steps of embodiment 3 preparing ZnCdS are:

[0044] Weigh 1 g Zn(Ac) 2 2H 2 O and 1.5 g Cd(NO 3 ) 2 4H 2 O In a round bottom flask, add 20 mL of ultrapure water and 1 mL of ammonia water, then slowly drop 5 mL of ionic liquid into the round bottom flask, sonicate the above mixed solution for 30 min, and dissolve another 2 g of sodium sulfide in 8 mL Ultrapure water and sonication for 30 min, after sonication, it was added dropwise to the round bottom flask under high-speed stirring, the solution turned yellow after 2 h of reaction, and then the mixed solution was transferred to a 50 mL reaction kettle, at 150 °C Calcined for 8 h, the product was washed three times with ultrapure water and absolute ethanol, and dried in a vacuum oven at 90 °C for 10 h, and then the product was naturally cooled to room temperature to obtain a yellow zinc-cadmium co-doped sulfide ZnCdS nanocrystals are dissolved in ultrapure water to obtain a ZnCdS suspension;

[0045] T...

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PUM

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Abstract

The invention relates to a preparation method and application of a photoelectrochemical thrombin aptamer sensor based on PS@Au dual inhibition of ZnCdS, belonging to the field of photoelectrochemical sensors. Zinc and cadmium co-doped sulfide ZnCdS nanocrystals were synthesized by a simple hydrothermal method. Its rough surface and large specific surface area greatly increased the loading capacity of ZnCdS and enhanced its visible light absorption capacity. Using ZnCdS as the substrate material and PS@Au as the secondary antibody label, PS@Au has good insulation, which hinders the transfer of photogenerated electrons, and the competition for the absorption of visible light between PS@Au and ZnCdS will also effectively reduce the light current signal, this dual suppression effect effectively improves the sensitivity of the sensor and reduces the detection limit of the sensor. Combined with the excellent selectivity of the appropriate ligand, the highly sensitive detection of the thrombin aptamer is realized, which is of great significance for the analysis and detection of thrombin.

Description

technical field [0001] The invention belongs to the fields of new functional nanomaterials, immunoassays and photoelectrochemical sensors, and provides a preparation method and application of a photoelectrochemical thrombin aptamer sensor based on PS@Au dual inhibition of ZnCdS. Background technique [0002] Thrombin is an extremely important protein that exists in mammals and has properties similar to hormones. It not only accelerates and consolidates the coagulation process, but also inhibits thrombus and activates platelets. Therefore, thrombin plays an important role in the diagnosis and treatment of cardiovascular diseases. The concentration of thrombin in blood is pmol L -1 Disease-related diagnoses, such as angiogenesis, tumor growth, and metastasis, can be performed within the scope. Therefore, the establishment of a highly sensitive and easy-to-operate detection method for thrombin will have far-reaching impacts in medicine and clinical practice. [0003] Current...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 范大伟鲍春竹刘昕张勇王欢吴丹马洪敏魏琴杜斌胡丽华
Owner UNIV OF JINAN
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