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Preparation method and application of photo-electrochemical thrombin aptamer sensor based on dual inhibition of PS@Au on ZnCdS

An aptamer sensor and photoelectrochemical technology, which is applied in the fields of new functional nanomaterials, immunoassays and photoelectrochemical sensors, can solve the problems of low detection sensitivity, easy to be affected by the environment, and complicated operation, so as to reduce the background signal, Good monodispersity performance and easy operation

Active Publication Date: 2018-11-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection methods have problems such as expensive instruments, complicated operation, easy to be affected by the environment, and low detection sensitivity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The specific steps for preparing ZnCdS are:

[0038] Weigh 1 g Zn(Ac) 2 2H 2 O and 1.5 g Cd(NO 3 ) 2 4H 2 O In a round bottom flask, add 20 mL of ultrapure water and 1 mL of ammonia water, then slowly drop 5 mL of ionic liquid into the round bottom flask, sonicate the above mixed solution for 30 min, and dissolve 2 g of sodium sulfide in 8 mL of ultrapure Ultrasound in pure water for 30 min, then add it dropwise into a round-bottomed flask under high-speed stirring after sonication, the solution turns yellow after 1 h of reaction, then transfer the mixture to a 50 mL reactor, and calcinate at 100 °C 8 h, the product was washed three times with ultrapure water and absolute ethanol, and dried in a vacuum oven at 90 °C for 10 h, and then the product was naturally cooled to room temperature to obtain a yellow zinc-cadmium co-doped sulfide ZnCdS nanocrystals are dissolved in ultrapure water to obtain a ZnCdS suspension;

[0039] The ionic liquid is 1-hexyl-3-...

Embodiment 2

[0040] The specific steps of embodiment 2 preparing ZnCdS are:

[0041] Weigh 2 g Zn(Ac) 2 2H 2 O and 3 g Cd(NO 3 ) 2 4H 2 O In a round bottom flask, add 40 mL of ultrapure water and 2 mL of ammonia water, then slowly drop 10 mL of ionic liquid into the round bottom flask, sonicate the above mixed solution for 40 min, and dissolve another 4 g of sodium sulfide in 16 mL of ultrapure Ultrasound in pure water for 40 min, then add it dropwise into a round-bottomed flask under high-speed stirring after sonication, the solution turns yellow after 1.5 h of reaction, then transfer the mixture to a 100 mL reactor, and calcinate at 120 °C 8 h, the product was washed three times with ultrapure water and absolute ethanol, and dried in a vacuum oven at 90 °C for 12 h, and then the product was naturally cooled to room temperature to obtain a yellow zinc-cadmium co-doped sulfide ZnCdS nanocrystals are dissolved in ultrapure water to obtain a ZnCdS suspension;

[0042] The ionic liquid ...

Embodiment 3

[0043] The specific steps of embodiment 3 preparing ZnCdS are:

[0044] Weigh 1 g Zn(Ac) 2 2H 2 O and 1.5 g Cd(NO 3 ) 2 4H 2 O In a round bottom flask, add 20 mL of ultrapure water and 1 mL of ammonia water, then slowly drop 5 mL of ionic liquid into the round bottom flask, sonicate the above mixed solution for 30 min, and dissolve 2 g of sodium sulfide in 8 mL of ultrapure Ultrasound in pure water for 30 min, then add it dropwise into a round-bottomed flask under high-speed stirring after sonication, the solution turns yellow after 2 h of reaction, then transfer the mixed solution to a 50 mL reactor, and calcinate at 150 °C 8 h, the product was washed three times with ultrapure water and absolute ethanol, and dried in a vacuum oven at 90 °C for 10 h, and then the product was naturally cooled to room temperature to obtain a yellow zinc-cadmium co-doped sulfide ZnCdS nanocrystals are dissolved in ultrapure water to obtain a ZnCdS suspension;

[0045] The ionic liquid is 1...

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PUM

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Abstract

The invention discloses a preparation method and application of a photo-electrochemical thrombin aptamer sensor based on dual inhibition of PS@Au on ZnCdS, and belongs to the field of photo-electrochemical sensors. Zinc and cadmium co-doped sulfides, namely ZnCdS nanocrystals, are synthesized through a simple hydrothermal method, and through the rough surface and the large specific surface area ofZnCdS, the loading capacity of the ZnCdS is greatly improved, and the visible light absorption capacity of the ZnCdS is enhanced; the ZnCdS serves as a substrate material, the PS@Au serves as a secondary antibody marker, the PS@Au has good insulativity, thus transfer of photo-generated electrons is impeded, photocurrent signals are also effectively reduced through competition between the PS@Au and the ZnCdS to absorption of visible light, the sensitivity of the sensor is effectively improved through the dual-inhibition effect, and the detection limit of the sensor is lowered; and by combiningthe excellent selectivity of an aptamer, highly-sensitive detection of the thrombin aptamer is achieved, and important significance for analysis and detection of thrombin is achieved.

Description

technical field [0001] The invention belongs to the fields of new functional nanomaterials, immunoassays and photoelectrochemical sensors, and provides a preparation method and application of a photoelectrochemical thrombin aptamer sensor based on PS@Au dual inhibition of ZnCdS. Background technique [0002] Thrombin is an extremely important protein that exists in mammals and has properties similar to hormones. It not only accelerates and consolidates the coagulation process, but also inhibits thrombus and activates platelets. Therefore, thrombin plays an important role in the diagnosis and treatment of cardiovascular diseases. The concentration of thrombin in blood is pmol L -1 Disease-related diagnoses, such as angiogenesis, tumor growth, and metastasis, can be performed within the scope. Therefore, the establishment of a highly sensitive and easy-to-operate detection method for thrombin will have far-reaching impacts in medicine and clinical practice. [0003] Current...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 范大伟鲍春竹刘昕张勇王欢吴丹马洪敏魏琴杜斌胡丽华
Owner UNIV OF JINAN
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