Surface-enhanced Raman scattering probe and preparation method thereof

A surface-enhanced Raman and probe technology, which is applied in the field of life analysis chemistry and nanomaterials, can solve the problems of low repeatability of the preparation method, high preparation cost, and complicated operation, so as to enhance the Raman signal and overcome the difficulty of repeatability Effect

Inactive Publication Date: 2012-07-18
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above deficiencies, the purpose of the present invention is to propose a surface-enhanced Raman scattering probe and its preparation method to solve the problems of low repeatability of the product manufacturing method, complicated operation, and high preparation cost. development of chemistry

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  • Surface-enhanced Raman scattering probe and preparation method thereof
  • Surface-enhanced Raman scattering probe and preparation method thereof
  • Surface-enhanced Raman scattering probe and preparation method thereof

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preparation example Construction

[0027] From the summary of the preparation method of the surface-enhanced Raman scattering probe, it mainly includes the following six steps: 1. Pre-preparing a noble metal with an aspect ratio between 2-6 and a surface plasmon absorption region between 500-2000nm wavelength range Nanorods; Ⅱ. React the Raman signal molecules with the noble metal nanorods prepared in step Ⅰ, so that the Raman signal molecules are adsorbed or coupled on the surface of the noble metal nanorods of the probe; Ⅲ. The noble metal nanorods prepared in step Ⅱ The surface forms an intermediate sandwich layer wrapping Raman signal molecules; IV, adding an amination solution to the ethanol solution dispersion system, and modifying the surface of the intermediate sandwich layer of the probe prepared in step III with amino functional groups, and simultaneously using tetrahydroxymethyl chloride Preparation of 1-3nm noble metal colloidal solution by phosphorous reduction method is used as the growth seed for ...

Embodiment 1

[0031] Embodiment 1 (the middle sandwich layer is silicon dioxide).

[0032] 1. Pre-preparation of gold nanorods (reference literature: Chem.Mater.2003, 15.1957): preparation of gold nanorods with an aspect ratio of 2-6 and a maximum ultraviolet absorption in the wavelength range of 500-2000nm.

[0033] (1) Preparation of seed solution: 5 mL of 0.5 mmol / L HAuCl 4 The solution was mixed with 5mL of cetyltrimethylammonium bromide (CTAB, 0.2mol / L) solution and stirred magnetically, then 0.6mL of newly prepared NaBH4 (0.01mmol / L) solution was added, and stirred at room temperature for 2min, and it was found that A yellow-brown gold colloid solution was used as a seed solution for growing gold nanorods.

[0034] (2) Growth of nano-gold rods: in a 50mL round bottom flask, add a certain volume of AgNO 3 (0.1mol / L) solutions (volumes of 0.05mL, 0.08mL, 0.12mL, 0.15mL and 0.18mL, and AgNO 3 The molar mass determines the aspect ratio of gold nanorods), then add 5mL of CTAB (0.2mol / L) ...

Embodiment 2

[0042] Embodiment 2 (the middle sandwich layer is polydielectric).

[0043] The same steps as in Example 1 will not be repeated here, but the differences are described in detail as follows: Step 3', after completing Step 2 of Example 1, dissolve the gold nanorods treated in Step 2 in 1.0 mM NaCl solution , add sodium styrene sulfonate (PSS) dissolved in NaCl (1.0mM) stock solution (10mg / ml), centrifuge after 30 minutes, and then disperse in 1.0mM NaCl solution, add polyacrylamine salt dissolved in NaCl (1.0mM) stock solution (10mg / ml) of PAH (PAH) was also centrifuged after 30 minutes, and repeated several times to form a sandwich layer of the polymer.

[0044] 4'. Mix the gold nanorod particles synthesized in step 3' with the gold seed solution synthesized in step 5 of Example 1, and make the gold seed nanoparticles adsorb on the surface of the polyelectrolyte polymer layer through physical electrostatic interaction.

[0045] 5', the nanomaterials processed in step 4' are ...

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Abstract

The invention relates to a surface-enhanced Raman scattering probe and a preparation method thereof. The probe is an interlayer structure and consists of a core precious-metal nano-rod, an intermediate sandwich layer and a precious-metal shell layer which grows and is formed on the outer surface, wherein a Raman signal molecule is wrapped inside the intermediate sandwich layer. The preparation method comprises the following steps that: the surface of the precious-metal nano-rod absorbs or is coupled with the Raman signal molecule, then the Raman signal molecule is wrapped to form the intermediate sandwich layer through silicon dioxide or polyelectrolyte, and then the precious-metal shell layer grows and is formed outside the intermediate sandwich layer. Through the surface-enhanced Raman scattering probe with the sandwich structure, strong local electromagnetic density is produced through the interaction of the surface plasma between the gold nano-rod and the outer metal shell layer, so the Raman signal is greatly enhanced, the weaknesses that a two-dimensional underlay is difficult to repeat, is expensive and is complicated can be overcome, and at the same time the size of the surface-enhanced Raman scattering probe is smaller than 200nm, biological detection of living bodies and application of biological imaging can be facilitated when the surface-enhanced Raman scattering probe is used as a biological probe.

Description

technical field [0001] The invention relates to the fields of nanomaterials and life analysis chemistry, in particular to a surface-enhanced Raman scattering (SERS) probe and a preparation method thereof. Background technique [0002] The signal intensity of surface-enhanced Raman spectroscopy is several to ten orders of magnitude higher than that of ordinary Raman spectroscopy, so surface-enhanced Raman scattering signals are used for the detection of extremely low concentrations or even single molecules, and when detecting organic molecules and biomolecules It shows the technical characteristics of being very simple, fast and cheap. Since Nie Shuming (science, 1997, 275, 1102-1106) reported single-molecule surface-enhanced Raman scattering (SERS) detection technology in 1997, especially Mirkin of Northwestern University in the United States (Science, 2002, 297, 1536-1540) and Since the group of Nie Shuming (Nat.Biotech, 2008, 26, 83-89) reported the SERS detection technol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 王新邹楠
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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