Enhanced Raman substrate for detecting streptomycete hyphae, and preparation and use methods thereof

Abstract

The invention provides an enhanced Raman substrate for detecting streptomycete hyphae, preparation and use methods thereof. The detection substrate is composed of a filter membrane layer (1), a monodisperse nanometer particle layer (2) and a metallic coating (3) from bottom to top. The detection substrate is high in detection speed and sensitivity. The preparation process is as follows: 1) self-assembling on the filter membrane layer (1), thereby acquiring the monodisperse nanometer particle layer (2); 2) preparing a gold nanoparticle layer on the monodisperse nanometer particle layer (2); and 3) taking gold nanoparticles in the gold nanoparticle layer as a nucleus for growing the metallic coating (3), thereby acquiring the detection substrate. The preparation method is simple and fast. The detection substrate can be used for detecting if a detected sample contains the streptomycete hyphae; and the Raman spectrum of the sample solution is collected for detecting if the streptomycete hyphae are contained in the detected sample and detecting the content of the streptomycete hyphae. The method has wide application prospects in the fields of clinical detection, inspection quarantine, environment monitoring, and the like.

Description

Technical field [0001] The invention relates to an enhanced Raman substrate for detecting Streptomyces hyphae and a preparation and use method thereof, belonging to the field of bacteria detection. Background technique [0002] Nosiheptide, also known as Nosihitide, Nopeptidin or Nopeptomycin, is a sulfur-containing peptide antibiotic. It was first discovered by French scientists in the fermentation broth of Streptomyces actllosl ZS 40037 in 1961, and then obtained in the soil of Argentina. A strain that synthesizes Nosiheptide, it is sensitive to most Gram-positive bacteria, especially Staphylococcus aureus, Streptococcus and Clostridium weichii. By hindering the protein synthesis of bacteria, it inhibits the growth of bacteria, so it can Prevent and treat respiratory diseases and necrotizing enteritis. At present, many countries and regions (such as the European Union, Japan, Taiwan, China, etc.) allow nosiheptide to be used as a feed additive. In 1998, my country approved nos...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods of microorganisms mainly include culture and dyeing observation method, immunological method, molecular biology method and mass spectrometry. Traditional microbial culture methods generally need to go through steps such as enrichment, separation and culture, staining or biochemical identification, and the whole detection process is cumbersome. , time-consuming, and heavy workload, it generally takes 4 to 7 days to get the test results. Immunological methods have the advantages of good specificity and high sensitivity, but due to the complexity of microbial serotypes, false positive results often occur in the test, and positive results need to be tested. Further tests confirmed

In recent years, molecular biology technology based on polymerase chain reaction (PCR) technology has developed very rapidly due to the advantages of strong specificity and high sensitivity, but some microorganisms will produce humic acid, which inhibits the PCR reaction, resulting in false positive results

The method of mass spectrometry has strong specificity and high sensitivity for microbial detection, but the cost is high, and it is not suitable for the detection of large batches of samples

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