Tumor mitochondrial targeting magnetic nanoprobe, and preparation method and application thereof

A mitochondrial and magnetic nanotechnology, applied in the field of biological probes, can solve the problems of folic acid photosensitivity and hydrophobicity limiting biological applications, and achieve the effects of high yield, mild conditions, and simple preparation methods

Inactive Publication Date: 2019-06-04
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the photosensitivity and hydrophobicity of folic acid limit its biological applications

Method used

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  • Tumor mitochondrial targeting magnetic nanoprobe, and preparation method and application thereof
  • Tumor mitochondrial targeting magnetic nanoprobe, and preparation method and application thereof
  • Tumor mitochondrial targeting magnetic nanoprobe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] (a) Fe 3 o 4 Preparation of @OA nanoparticles

[0101] 2g Fe(OAc) 3 , 25ml of diphenyl ether, and 25ml of oleylamine (Oleyamine, OA) were mixed and dissolved in a 100ml three-necked flask, and the mixture was reacted under the protection of nitrogen; first, the excess water in the reaction was removed by heating at 120°C for 0.5h with an electric heating mantle, and then The temperature was raised to 280°C at a rate of 10°C / min, and the reaction was stirred for 1 hour, then the heating was stopped, and the solution was cooled to room temperature.

[0102] Add 100 mL of ethanol to the solution to dissolve the generated Fe 3 o 4 The nanoparticles were precipitated, and then centrifuged at a speed of 8000rpm for 10min to obtain Fe 3 o 4 @OA particles, dispersed in chloroform for use, the concentration is about 20mg / mL.

[0103] (b) PSMA-PEG 1k -NH 2 preparation

[0104] Will NH 2 -PEG 1k-NH-Boc 135.3mg and PMSA 190mg were dissolved in 5ml DMSO solution, and the...

Embodiment 2

[0115] (a) Fe 3 o 4 Preparation of @OA nanoparticles

[0116] 3g Fe(OAc) 3 , 35ml of diphenyl ether and 35ml of oleylamine (Oleyamine, OA) were mixed and dissolved in a 100ml three-necked flask, and the mixture was reacted under the protection of nitrogen; firstly, the excess water in the reaction was removed by heating at 120°C for 0.5h with an electric heating mantle, and then The temperature was raised to 280°C at a rate of 10°C / min, and the reaction was stirred for 1 hour, then the heating was stopped, and the solution was cooled to room temperature.

[0117] Add 100 mL of ethanol to the solution to dissolve the generated Fe 3 o 4 The nanoparticles were precipitated, and then centrifuged at a speed of 8000rpm for 10min to obtain Fe 3 o 4 @OA particles, dispersed in chloroform for use, the concentration is about 20mg / mL.

[0118] (b) PSMA-PEG 1k -NH 2 preparation

[0119] Will NH 2 -PEG 1k -NH-Boc 180mg and PMSA 200mg were dissolved in 5ml DMSO solution, and the...

Embodiment 3

[0129] (a) Fe 3 o 4 Preparation of @OA nanoparticles

[0130] 1g Fe(OAc) 3 , 25ml of diphenyl ether, and 20ml of oleylamine (Oleyamine, OA) were mixed and dissolved in a 100ml three-necked flask, and the mixture was reacted under the protection of nitrogen; first, the excess water in the reaction was removed by heating with an electric heating mantle at 120°C for 0.5h, and then The temperature was raised to 280°C at a rate of 10°C / min, and the reaction was stirred for 1 hour, then the heating was stopped, and the solution was cooled to room temperature.

[0131] Add 100 mL of ethanol to the solution to dissolve the generated Fe 3 o 4 The nanoparticles were precipitated, and then centrifuged at a speed of 8000rpm for 10min to obtain Fe 3 o 4 @OA particles, dispersed in chloroform for use, the concentration is about 20mg / mL.

[0132] (b) PSMA-PEG 1k -NH 2 preparation

[0133] Will NH 2 -PEG 1k -NH-Boc 120mg and PMSA 150mg were dissolved in 5ml DMSO solution, and the ...

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Abstract

The invention provides a tumor mitochondrial targeting magnetic nanoprobe, and a preparation method and an application thereof. In the preparation method of the tumor mitochondrial targeting magneticnanoprobe provided by the invention, cyanine dye molecules are compounded on the surface of Fe3O4 nanoparticles by covalent crosslinking, so that the prepared nanoprobe has good stability, monodispersity and good biocompatibility and targeting effect, and can be used not only in biological in-vivo imaging, but also as tumor diagnostic and therapeutic preparations, carriers and magnetic separationmaterials. At the same time, the preparation method is simple in process and suitable for promotion and large-scale production.

Description

technical field [0001] The invention relates to the field of biological probes, in particular to a tumor mitochondria-targeting magnetic nanoprobe, a preparation method and application thereof. Background technique [0002] Due to the rapid development of imaging and the emergence of various imaging methods in recent years, the accuracy of disease diagnosis in medicine is increasingly required. Diversification and other advantages, the development is extremely rapid. [0003] The use of polymethine cyanine fluorescent small molecules in near-infrared fluorescence imaging has the advantages of low background interference, high sensitivity, and strong tissue penetration. In addition to sensitivity, the core requirement for a probe is the specificity of its development. In order to make polymethine cyanine fluorescent small molecules have tumor-targeting imaging properties, in recent years, many researchers have combined them with tumor-targeting ligands, such as folic acid, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/10A61K47/02A61K47/22A61P35/00
Inventor 蔡林涛周理华龚萍孟晓青张佳丽李文军邓冠军
Owner SHENZHEN INST OF ADVANCED TECH
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