31results about "Material analysis using immobilised reagents" patented technology

Compositions, articles, systems, and methods for sensing and / or authentication using luminescence imaging are generally provided. In some embodiments, the article is associated with an emissive material comprising an emissive species. In some embodiments, the emissive species has a radiative lifetime of at least 10 nanoseconds. A characteristic of the article may be determined by acquiring an image comprising time-dependent information related to the emissive species. In certain cases, the radiative lifetime of the emissive species may be determined from the image. Because the radiative lifetime of an emissive species may be modified by numerous factors, including, but not limited to, binding to or proximity to other molecules, temperature, pH, and radiation exposure, the measured length of the radiative lifetime may provide information regarding a characteristic of the associated article. In some cases, the emissive material comprising one or more emissive species may be used to identify and / or authenticate the associated article.

Provided herein are methods, systems and digital microfluidic cartridges for the determination of ligand-target analyte affinity value (KD), determination of an association rate constant (kon), and / or determination of a dissociation rate constant (koff) in a PR (plasmon resonance) based system. More specifically, as described herein, the present disclosure is directed to improved methods, systems and digital microfluidic cartridges for determining a dissociation rate constant (koff) using free ligand molecules in the dissociation phase or dissociation step of a standard kinetic binding curve or in ligand-target analyte affinity analysis (referred to herein as ligand assisted dissociation (LAD)).

A sensor chip for detecting a presence of chemical, biochemical or biological particles to be sensed in an analyte fluid comprises at least one cavity extending from the back side of an SOI substrate through its base and buried oxide layers to expose a back surface of the silicon layer. At least one monolithically integrated lateral photodiode comprises a lateral layer sequence of active photodiode layers, forming an integral part of the exposed back surface defining a photosensor surface. A functionalized coating either comprises a capturing agent, a preloaded capturing agent with labelled analyte combination or is configured to immobilize a capturing agent upon exposure of the surface functionalization layer to the capturing agent, for selectively capturing the particles to be sensed from an analyte fluid upon exposure of the functionalization layer to the analyte fluid. The photodiode is sensitive to detect, upon capture of one or more of the particles to be sensed by the capturing agent, a characteristic light emission or a characteristic change in light emission from the surface functionalization layer in response to supplied excitation energy.

Various embodiments of devices comprising a synthetic polymer substrate having a high quality finish upper surface having at least a double layer coating comprising a first reflective layer and a second transparent layer are disclosed. Also disclosed are embodiments of the disclosed devices and kits that can detect particles. Also disclosed are various embodiments of methods of using the disclosed devices and various embodiments of methods of using the disclosed kits.

A sensor chip for detecting a presence of chemical, biochemical or biological particles to be sensed in an analyte fluid comprises at least one cavity extending from the back side of an SOI substrate through its base and buried oxide layers to expose a back surface of the silicon layer. At least one monolithically integrated lateral photodiode comprises a lateral layer sequence of active photodiode layers, forming an integral part of the exposed back surface defining a photosensor surface. A functionalized coating either comprises a capturing agent, a preloaded capturing agent with labelled analyte combination or is configured to immobilize a capturing agent upon exposure of the surface functionalization layer to the capturing agent, for selectively capturing the particles to be sensed from an analyte fluid upon exposure of the functionalization layer to the analyte fluid. The photodiode is sensitive to detect, upon capture of one or more of the particles to be sensed by the capturing agent, a characteristic light emission or a characteristic change in light emission from the surface functionalization layer in response to supplied excitation energy.

Systems and methods for soak imaging of a flow cell. In one example, an imaging system includes liquid ports positioned relative to front and rear ends of a scanning imaging objective. A front end liquid port distributes soak solution to the space between the distal end of the imaging objective and the cover of the flow cell, and a rear end liquid port collects soak solution. In another example, an imaging system includes a flow cell having a cover movable relative to a substrate. The system is configured to move the imaging objective and the flow cell cover as a unit relative to the flow cell substrate.

Disclosed are embodiments photonic integrated circuits with photo-switchable components. Embodiments are illustrated in the form evanescent field perturbation sensors in which receptor molecules are patterned onto the sensor substrate with precision. In particular, light patterning and molecular-based photo-responsive motifs allow for the positioning of receptor molecules on the operational elements of the evanescent field perturbation sensors. Receptor molecules are further detachable from the sensor and capable of replacement with additional receptor molecules of the same type, or one or more alternative receptor molecules capable of receiving the same or different analyte receptor molecules. In certain embodiments the light sources for patterning and receptor molecule interchange may integrated within the sensor and / or sensor platform itself. In certain embodiments a method of recharging and / or reconfiguring the receptor molecules, and a machine for carrying out the method is disclosed.

5 This invention provides an apparatus for monitoring chemical reactions, the apparatus comprising at least one layer adapted to excite surface phonon polariton, at least one layer adapted to excite localized surface plasmon polariton, and a substrate, wherein the layers are stacked alternately on the substrate, and are further adapted to exhibit a plasmon-10 phonon coupling mode upon exposure to radiation for vibrational fingerprinting of molecules from the monitored chemical reactions based on their refractive index. The invention further provides a system and method involving the apparatus. 1 for publication. 15

A sensor chip for detecting a presence of chemical, biochemical or biological particles to be sensed in an analyte fluid comprises at least one cavity extending from the back side of an SOI substrate through its base and buried oxide layers to expose a back surface of the silicon layer. At least one monolithically integrated lateral photodiode comprises a lateral layer sequence of active photodiode layers, forming an integral part of the exposed back surface defining a photosensor surface. A functionalized coating either comprises a capturing agent, a preloaded capturing agent with labelled analyte combination or is configured to immobilize a capturing agent upon exposure of the surface functionalization layer to the capturing agent, for selectively capturing the particles to be sensed from an analyte fluid upon exposure of the functionalization layer to the analyte fluid. The photodiode is sensitive to detect, upon capture of one or more of the particles to be sensed by the capturing agent, a characteristic light emission or a characteristic change in light emission from the surface functionalization layer in response to supplied excitation energy.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.

A device configured to provide a value indicative of fluorescent emission from a substrate 5 having a test region is provided. The device comprises an electromagnetic radiation source configured to emit excitation radiation towards the test region to excite fluorescent emission from a fluorescent material in the test region. The electromagnetic radiation source is configured such that a variation in intensity of the excitation radiation across the test region is less than 15%. The device further comprises a sensor configured to capture a primary 10 image of the fluorescent emission, and a controller configured to modify the primary image based on calibration data and to use the modified image to obtain the value indicative of the fluorescent emission.

Provided herein is a lateral flow test device for determining the amount of a plurality of hormones in a test sample such as a blood sample. The lateral flow test device is suitable for self-testing and provides rapid and accurate results.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.

Disclosed are embodiments photonic integrated circuits with photo-switchable components. Embodiments are illustrated in the form evanescent field perturbation sensors in which receptor molecules are patterned onto the sensor substrate with precision. In particular, light patterning and molecular-based photo-responsive motifs allow for the positioning of receptor molecules on the operational elements of the evanescent field perturbation sensors. Receptor molecules are further detachable from the sensor and capable of replacement with additional receptor molecules of the same type, or one or more alternative receptor molecules capable of receiving the same or different analyte receptor molecules. In certain embodiments the light sources for patterning and receptor molecule interchange may integrated within the sensor and / or sensor platform itself. In certain embodiments a method of recharging and / or reconfiguring the receptor molecules, and a machine for carrying out the method is disclosed.

Compositions, articles, systems, and methods for sensing and / or authentication using luminescence imaging are generally provided. In some embodiments, an article (or packaging material of an article) is associated with an emissive material comprising an emissive species (e.g., a luminescent species). In some cases, the emissive species has an emission lifetime of at least 10 nanoseconds (ns). In some cases, a characteristic of an article (e.g., identity, authenticity, age, quality, purity) may be determined by obtaining an image (or series of images) comprising time-dependent information related to an emissive species. In certain instances, for example, the emission lifetime of an emissive species may be determined from an image (or series of images). Since the emission lifetime of an emissive species may be modified by a number of factors, including but not limited to binding or proximity to other molecules (e.g., water, oxygen, carbon monoxide), temperature, pH, and radiation exposure, the measured length of the emission lifetime (e.g., the observed emission lifetime, the emission time period) may provide information regarding a characteristic of an associated article. In some instances, an emissive material comprising one or more emissive species may be used to identify and / or authenticate an associated article.

A biosensor for detecting the presence, amount, or binding kinetics of an analyte bound to an analyte binding molecule is provided. The biosensor includes a core component and a bacteriophage (such as an M13 bacteriophage) displaying the analyte binding molecule immobilized thereon. Also provided are methods for detecting or quantitating the presence or amount of an analyte bound to an analyte binding molecule, or binding kinetics, using the biosensor having an analyte binding molecule-displaying bacteriophage immobilized thereon. The biosensor can be a bio-layer interferometry (BLI) biosensor, and can detect the presence, amount, or binding kinetics of the analyte (e.g., antigen) binding to a plurality of phage-displayed analyte binding molecules (e.g., antibodies) efficiently and rapidly.

A multi-reflection liquid-immersion silicon-based microfluidic measurement device and measurement method provided by one embodiment of the present invention completely separates the first reflected light reflected from the sample detection layer from the second reflected light reflected from the prism-buffer solution interface through multiple reflections, and amplifies the measurement sensitivity by achieving multiple incidences through multiple reflections. The multi-reflection liquid-immersion silicon-based microfluidic measurement device of an embodiment of the present invention includes: a microfluidic structure, consisting of a support and at least one microfluidic path, the microfluidic path being formed on the support, a sample detection layer being formed in the microfluidic path, and a bioconjugate for detecting the sample being fixed in the sample detection layer; a sample injection portion for injecting a buffer solution containing the sample into the microfluidic path; a prism unit, provided with a prism and a reflector, the reflector being formed by coating a reflective mirror surface on the bottom surface of the prism; a polarized light generating portion for generating polarized light; and a polarized light detecting portion for detecting polarization changes of the reflected light.

The present disclosure provides methods, devices, and systems for fluorescence-based analyte detection. Devices may include a surface layer configured to be in contact with a solution. The surface layer may include an immobilized capture probe configured to bind an analyte. The device may include a photodiode transducer, current switch, or circuitry. The photodiode transducer may include a first photodiode disposed adjacent to a second photodiode. The current switch may divert current to a high gain detection path or a low gain detection path. Methods may include using the devices and systems described herein for analyte detection.

A novel combinatorial and high-throughput platform that enables rapid screening of complex and heterogeneous copolymer brushes as enzyme immobilization supports named Combinatorial High-throughput Enzyme Support Screening (CHESS). Using a 384 well-plate format, we synthesized arrays of three-component polymer brushes in the microwells using photo-activated surface-initiated polymerization, and immobilized enzymes in situ. The utility of CHESS to identify optimal immobilization supports under thermally and chemically denaturing conditions was demonstrated using Bacillus subtilis Lipase A (LipA). The identification of supports with optimal compositions was validated by immobilizing LipA on polymer-brush modified biocatalyst particles. We further demonstrated that CHESS could be used to predict the optimal composition of polymer brushes a priori for the previously unexplored enzyme, alkaline phosphatase (AlkP). Our findings demonstrate that CHESS represents a predictable and reliable platform for dramatically accelerating the search of chemical compositions for immobilization supports and further facilitate the discovery of biocompatible and stabilizing materials.

An irradiation device and a photometric device include a first light-emitting element and a second light-emitting, in which a relative positional relationship between the first light-emitting element and the second light-emitting element satisfies a first condition which is that the first and the second light-emitting elements are arranged at an interval of 90°˜180° as a central angle around the Z axis, and the second condition which is that one of a first intersection and a second intersection is located in a positive direction and another is located in a negative direction, where the first intersection intersects between a first light emission center axis and the Z axis, the second intersection intersects between a second light emission center axis and the Z axis, and the positive direction and the negative direction are respectively on one side and another side on the Z axis with respect to the XY plane.

The present invention discloses a dry chemical enzyme method uric acid detection reagent card, which effectively solves the technical problems of complicated, low-efficiency and inaccurate uric acid detection in the existing technology. The reagent card comprises a PVC back plate, on which a sample processing section, an enzymatic reaction section, a substrate mixing section and an enzymatic detection section are sequentially adhered from left to right. A card shell is arranged above the reagent card, and a sample addition hole arranged above the sample processing section and a detection hole arranged above the enzymatic detection section are arranged on the card shell. The present invention is exquisitely designed, and utilizes chromatography power. The blood sample sequentially passes through the sample processing section to remove red blood cells, is catalytically decomposed in the enzymatic reaction section, and is mixed with a detection substrate in the substrate mixing section, so as to achieve final color development or luminescence in the detection section. The chromatography filter paper used in the detection section is both the power of sample chromatography and the carrier for collecting reaction products for final detection.

Analyte binding to analyte binding moieties such as oligos on an array such as a surface is disclosed. Binding is assayed using, for example, fluorophore labeled probes rather than using fluorophores on the analyte binding moieties, resulting in a substantial increase in system efficiency.

Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.

Disclosed are embodiments photonic integrated circuits with photo-switchable components. Embodiments are illustrated in the form evanescent field perturbation sensors in which receptor molecules are patterned onto the sensor substrate with precision. In particular, light patterning and molecular-based photo-responsive motifs allow for the positioning of receptor molecules on the operational elements of the evanescent field perturbation sensors. Receptor molecules are further detachable from the sensor and capable of replacement with additional receptor molecules of the same type, or one or more alternative receptor molecules capable of receiving the same or different analyte receptor molecules. In certain embodiments the light sources for patterning and receptor molecule interchange may integrated within the sensor and / or sensor platform itself. In certain embodiments a method of recharging and / or reconfiguring the receptor molecules, and a machine for carrying out the method is disclosed.

Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.

An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed.

The present invention relates to a biosensor system for detecting whether a target analyte is present in a fluid sample. The biosensor system comprises a carrier substrate and a biosensor. The carrier substrate has a carrier substrate front side and a carrier substrate back side and comprises a fluid sample reservoir that is accessible from the carrier substrate front side and configured for accommodating the fluid sample. The biosensor comprises a front-end-of-line, FEOL, and a back-end-of-line, BEOL. The biosensor is mounted with its FEOL in direct contact with the carrier substrate on the carrier substrate front side. The FEOL comprises at least one detector component that is arranged and configured for detecting whether the fluid sample reservoir accommodates a fluid sample that contains the target analyte and for providing an output signal indicative of whether the target analyte is present in the fluid sample.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.

Examples herein include an apparatus having a substrate, a sensor over the substrate including an active surface and a sensor bond pad, a molding layer over the substrate and covering sides of the sensor, the molding layer having a lower portion with a first molding height relative to a top surface of the substrate and an upper portion with a second molding height relative to the top surface of the substrate, the first molding height and the second molding height each being greater than a height of the active surface, the second molding height being great than the first molding height; and a lidding layer over at least some of the lower portion of the molding layer and over the active surface. The lidding layer and the molding layer form a space over the active surface of the sensor that defines a flow channel.