10results about "Material analysis using immobilised reagents" patented technology

Provided herein are methods, systems and digital microfluidic cartridges for the determination of ligand-target analyte affinity value (KD), determination of an association rate constant (kon), and / or determination of a dissociation rate constant (koff) in a PR (plasmon resonance) based system. More specifically, as described herein, the present disclosure is directed to improved methods, systems and digital microfluidic cartridges for determining a dissociation rate constant (koff) using free ligand molecules in the dissociation phase or dissociation step of a standard kinetic binding curve or in ligand-target analyte affinity analysis (referred to herein as ligand assisted dissociation (LAD)).

A sensor chip for detecting a presence of chemical, biochemical or biological particles to be sensed in an analyte fluid comprises at least one cavity extending from the back side of an SOI substrate through its base and buried oxide layers to expose a back surface of the silicon layer. At least one monolithically integrated lateral photodiode comprises a lateral layer sequence of active photodiode layers, forming an integral part of the exposed back surface defining a photosensor surface. A functionalized coating either comprises a capturing agent, a preloaded capturing agent with labelled analyte combination or is configured to immobilize a capturing agent upon exposure of the surface functionalization layer to the capturing agent, for selectively capturing the particles to be sensed from an analyte fluid upon exposure of the functionalization layer to the analyte fluid. The photodiode is sensitive to detect, upon capture of one or more of the particles to be sensed by the capturing agent, a characteristic light emission or a characteristic change in light emission from the surface functionalization layer in response to supplied excitation energy.

Disclosed are embodiments photonic integrated circuits with photo-switchable components. Embodiments are illustrated in the form evanescent field perturbation sensors in which receptor molecules are patterned onto the sensor substrate with precision. In particular, light patterning and molecular-based photo-responsive motifs allow for the positioning of receptor molecules on the operational elements of the evanescent field perturbation sensors. Receptor molecules are further detachable from the sensor and capable of replacement with additional receptor molecules of the same type, or one or more alternative receptor molecules capable of receiving the same or different analyte receptor molecules. In certain embodiments the light sources for patterning and receptor molecule interchange may integrated within the sensor and / or sensor platform itself. In certain embodiments a method of recharging and / or reconfiguring the receptor molecules, and a machine for carrying out the method is disclosed.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.

Provided herein is a lateral flow test device for determining the amount of a plurality of hormones in a test sample such as a blood sample. The lateral flow test device is suitable for self-testing and provides rapid and accurate results.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.

A biosensor for detecting the presence, amount, or binding kinetics of an analyte bound to an analyte binding molecule is provided. The biosensor includes a core component and a bacteriophage (such as an M13 bacteriophage) displaying the analyte binding molecule immobilized thereon. Also provided are methods for detecting or quantitating the presence or amount of an analyte bound to an analyte binding molecule, or binding kinetics, using the biosensor having an analyte binding molecule-displaying bacteriophage immobilized thereon. The biosensor can be a bio-layer interferometry (BLI) biosensor, and can detect the presence, amount, or binding kinetics of the analyte (e.g., antigen) binding to a plurality of phage-displayed analyte binding molecules (e.g., antibodies) efficiently and rapidly.

Systems and methods for immersion imaging of a flowcell. In one example, an imaging system includes liquid ports in leading and trailing positions relative to a scanned imaging objective. The leading liquid port dispenses an immersion liquid into a space between the distal end of the imaging objective and the flowcell's cover, with the trailing liquid port collecting the immersion liquid. In another example, an imaging system includes a flowcell with a cover that is moveable relative a substrate. The system is configured to move the imaging objective and flowcell cover as a unit relative to the flowcell substrate.

Disclosed are embodiments photonic integrated circuits with photo-switchable components. Embodiments are illustrated in the form evanescent field perturbation sensors in which receptor molecules are patterned onto the sensor substrate with precision. In particular, light patterning and molecular-based photo-responsive motifs allow for the positioning of receptor molecules on the operational elements of the evanescent field perturbation sensors. Receptor molecules are further detachable from the sensor and capable of replacement with additional receptor molecules of the same type, or one or more alternative receptor molecules capable of receiving the same or different analyte receptor molecules. In certain embodiments the light sources for patterning and receptor molecule interchange may integrated within the sensor and / or sensor platform itself. In certain embodiments a method of recharging and / or reconfiguring the receptor molecules, and a machine for carrying out the method is disclosed.

A downhole fluid sampling tool may include an optical measurement tool and a viewing region disposed in the optical measurement tool. In examples, a bridge may be disposed in a transparent portion of the flow path between a light source and a light modifier and an optical detector. The bridge includes a structure comprising a substrate and a contrast agent, wherein the contrast agent is any molecule configured to interact with an analyte and alter a property of the analyte and / or contrast agent, wherein the property is detectable by the optical measurement tool.