70results about "Material analysis using immobilised reagents" patented technology

A device, system and method for portable fluorescence detection. The portable device of the present invention features a low power light, in which a wavelength range is defined as at least one wavelength of light. The light source is preferably highly energy efficient, such that a majority of the electrical power which is consumed is then converted into transmitted light. The emitted light from the excited fluorophore is then preferably detected with any low cost and low power photodetector. Although optionally a highly sensitive optical detector may be used, preferably fluorescence is detected with any light sensing device, such as a regular photodiode or a CCD (charge-coupled device) sensor for example.

A change in mass of a microbridge in a mass sensor can be sensed by applying a time-varying amplitude modulated electrostatic force to excite the microbridge into resonance at the frequency of amplitude modulation. An optical energy is then transmitted at a wavelength close to a resonant wavelength of a Fabry-Perot microcavity, which is formed by etching a movable reflective mirror into a region of the microbridge and by etching a fixed reflective minor in a region spaced apart from the microbridge. The two mirrors are interconnected by an optical waveguide. The movable mirror and fixed mirror reflect the optical energy to a receiver, and a change in the Fabry-Perot microcavity's reflectivity is interferometrically determined. The change in reflectivity indicates a change in the microbridge's resonant frequency due to increased mass of the microbridge resulting from sorption of a target chemical by a layer of chemoselective material deposited on the microbridge.

Methods and devices for the measurement of molecular binding interactions. Preferred embodiments provide real-time measurements of kinetic binding and disassociation of molecules including binding and disassociation of protein molecules with other protein molecules and with other molecules. In preferred embodiments ligands are immobilized within pores of a porous silicon interaction region produced in a silicon substrate, after which analytes suspended in a fluid are flowed over the porous silicon region. Binding reactions occur when analyte molecules diffuse closely enough to the ligands to become bound. Preferably the binding and subsequent disassociation reactions are observed utilizing a white light source and thin film interference techniques with spectrometers arranged to detect changes in indices of refraction in the region where the binding and disassociation reactions occur. In preferred embodiments both ligands and analytes are delivered by computer controlled robotic fluid flow control techniques to the porous silicon interaction regions through microfluidic flow channels.

A phase contrast quadrature interferometric method for determining the presence or absence of a target analyte in a sample. The method comprises using a laser beam having a wavelength λ and a waist wo to probe at least a portion of a substrate having a reflecting surface that has been exposed to the sample. The reflecting surface includes at least a first region having a layer of recognition molecules specific to the target analyte and a second region that does not include a layer of recognition molecules specific to the target analyte. The method further comprises measuring a time dependent intensity on a photodetector of a substantially only first quadrature at one of a pair of quadrature angles ⊖q of a reflected diffraction signal of the probe beam while probing the first region and the second region. An apparatus for phase-contrast quadrature interferometric detection of the presence or absence of a target molecule on a planar array, comprises a laser source for generating a probe beam. The apparatus includes a platform for receiving the planar array and a first optical train for directing the probe beam at the platform in a substantially surface normal manner. The apparatus also includes an objective lens having a first side and a second side and having a focal length, the objective lens being offset on the first side of the lens from the platform by a first distance approximately equal to the focal length. The apparatus further includes split photodetector means for measuring a first quadrature and a second quadrature in a signal resulting from reflection of the probe laser beam.

A symmetric or asymmetric multilayer structure based on the technique of surface plasmon resonance (SPR) has been applied for modulation of resonant angle and wavelength. The fabrication of this invention can have nanoscale thin film layers up to several hundreds, while each layer has its own material of a high or low refractive index value, and the total layers in a thickness of tens to hundreds nanometers are grown in this single structure. This invention is intended for optimizing the scanning of mechanism by modulating SPR resonant angle and wavelength, and for developing the prospect of portable instruments.

A change in mass of a microbridge in a mass sensor can be sensed by applying a time-varying amplitude modulated electrostatic force to excite the microbridge into resonance at the frequency of amplitude modulation. An optical energy is then transmitted at a wavelength close to a resonant wavelength of a Fabry-Perot microcavity, which is formed by etching a movable reflective mirror into a region of the microbridge and by etching a fixed reflective minor in a region spaced apart from the microbridge. The two mirrors are interconnected by an optical waveguide. The movable mirror and fixed mirror reflect the optical energy to a receiver, and a change in the Fabry-Perot microcavity's reflectivity is interferometrically determined. The change in reflectivity indicates a change in the microbridge's resonant frequency due to increased mass of the microbridge resulting from sorption of a target chemical by a layer of chemoselective material deposited on the microbridge.

The invention discloses a biosensor based on a Ti3C2 two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and a preparation method thereof. The biosensor based comprises a probe and a biosensor electrode, wherein the probe comprises nanosheets Ti3C2 MXenes, joint molecules and biometric molecules 1; the nanosheets Ti3C2 MXenes are connected with the joint molecules through electrostatic adsorption; the joint molecules are connected with the biometric molecules 1 through amide groups; the joint molecules contain primary or secondary amine groups; the joint molecules can be positively charged after being dissolved in water; the biometric molecules 1 are single-stranded DNA sequences 1 having carboxyl groups at the 5' ends; the single-stranded DNA sequences 1 can recognize CD63 protein on exosomes. The invention finds that Ti3C2 MXenes can improve the electrochemiluminescence of luminol for the first time, and by virtue of the property, the Ti3C2 MXenes is prepared into the probe and then the biosensor is prepared.

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.

A mass sensor system including multiple Fabry-Perot microcavities connected in parallel by multiple waveguides. Each of the mass sensors includes a microbridge having a fundamental resonance frequency, and a movable reflective mirror etched into the microbridge; a fixed reflective mirror etched in a substrate, the fixed reflective mirror being fixed to the substrate in a region spaced apart from the movable reflective mirror; and an optical waveguide etched in the substrate that connects the movable mirror and the fixed mirror forming the Fabry-Perot microcavity interferometer. The system includes a tunable continuous-wave laser operative to optically interrogate the Fabry-Perot microcavity of each of the plurality of mass sensors, and a receiver operative to receive sensor signals from each of the plurality of mass sensors, the sensor signals comprising reflective signals and transmitted signals. A continuous-wave laser may generate optical forces that modify the motion, dynamics, or mechanical Q-factor of the microbridge.

Polydiacetylenes (PDAs) and PDA / ZnO nanocomposites based on the monomers: 10,12-pentacosadiynoic acid (PCDA), 10,12-tricosadiynoic acid (TCDA) and 10,12-docosadiynedioic acid (DCDA) monomers are chromatic chemical sensing agents for selected organic liquids. Thermochromically reversible compositions include PCDA and nanosize ZnO having a particle size range less than 100 nm.

The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.

Differing from conventional lateral flow immunoassay test strips, the present invention provides an immunoassay kit comprising a release strip and a reaction strip. When using this immunoassay kit to complete a competitive-type immunochromatographic test or a sandwich-type immunochromatographic test for a test sample, it needs to firstly put the release strip into the test sample for releasing a specific antibody (or antigen) conjugating with a marker into the test sample, so as to make the first antibody connect to a target object in the test sample; after that, the reaction strip is eventually put into the test sample after the release strip is removed out of the test sample. Therefore, the qualitative analysis result of the competitive / sandwich-type immunochromatographic test can be easily obtained by determining whether the test line shows the color reaction or not.

The invention relates to a Pd quantum dot-modified TiO2 nanorod-based photoelectric chemical analysis method and application thereof. By means of making a relational diagram of the concentration of a standard PCB 101 solution and current, the concentration of PCB 101 in a to-be-measured solution can be obtained. Compared with the prior art, by constructing a Pd quantum dot-modified TiO2 nanorod with efficient photoelectric catalytic reduction activity, the method realizes efficient reduction for the PCB 101, obtains sensitive cathode photocurrent and realizes photoelectric chemical detection for the PCB 101; furthermore, due to the introduction of molecularly imprinted loci and the establishment of a reaction type analysis method, the selectivity of the analysis method is further improved, and the interference of coexisting easily oxidized pollutants in environmental water with PCB 101 detection is greatly reduced. The method is simple and easy to implement and high in sensitivity, has a limit of detection reaching up to 10-13mol / L, is stable in electrodes and has good reproducibility.

A symmetric or asymmetric multilayer structure based on the technique of surface plasmon resonance (SPR) has been applied for modulation of resonant angle and wavelength. The fabrication of this invention can have nanoscale thin film layers up to several hundreds, while each layer has its own material of a high or low refractive index value, and the total layers in a thickness of tens to hundreds nanometers are grown in this single structure. This invention is intended for optimizing the scanning of mechanism by modulating SPR resonant angle and wavelength, and for developing the prospect of portable instruments.

Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N NaOH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

An electrogenerated chemiluminescence (ECL) probe is based on trititanium dicarbide two-dimensional (2D) metal carbide catalyzed luminol and a preparation method. The biosensor includes the probe and the electrode of the biosensor, wherein the probe includes the Ti3C2 MXenes nanosheets, a linker molecule and a bio-recognition molecule 1; the Ti3C2 MXenes nanosheets are linked with the linker molecule by electrostatic adsorption; the linker molecule is linked with the bio-recognition molecule 1 by an amide group, contains a primary or secondary amine group, and presents positive potential in water; the bio-recognition molecule 1 is a single-stranded DNA sequence 1 having a carboxyl group at the 5′ end, and a CD63 protein on exosomes is recognized by the single-stranded DNA sequence 1. It was found for the first time that Ti3C2 MXenes can improve the ECL signal of luminol, the Ti3C2 MXenes could be applicable to the ECL probe.

A rapid diagnostic test device uses driven flow technology to significantly expedite the testing time of a sample. The rapid diagnostic test device can be used to analyze liquids, such as some body fluids, by using labeled molecular affinity binding, such as immunochromatography. The test device can detect an analyte, such as an antibody or antigen, which may indicate a particular condition, the presence of a particular drug, or the like. The device includes a cover and base that combine to form an internal cavity for receiving a test strip. Compression bars in the cover and a compression cushion in the base sandwich a conjugate pad of the test strip to accelerate chemical mixtures to flow from the conjugate pad of the test strip toward fixed sites along the strip from which readings are taken.

The present invention relates to a device and a method for qualitative and quantitative analysis of heavy metals and more particularly provides a device and a method for qualitative and quantitative analysis of heavy metals utilizing a rotary disc system.

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.

The present invention provides an immune detection kit, which is mainly composed of a release reagent plate and a reaction reagent plate. The immune detection kit is different from lateral flow immunoassay test paper in the prior art, when the immune detection kit is used for competitive or sandwich type lateral flow immunoassay of a sample to be tested, first, the release reagent plate must be placed into a sample liquid for release into the sample liquid and further mutual combination with a target detection matter in the sample liquid of a marker-bonded antibody (or drug) which is carried by the release reagent plate; and finally the reaction reagent plate is placed into the sample liquid, so that the sample liquid and an antibody which is carried by a test line on the reaction reagent plate compete or combine with each other, and whether a test result is positive or negative can be judged by whether the test line shows color or not.

The present disclosure comprises a device and accompanying method for determining the presence or absence of Methicillin-resistant Staphylococcus aureus in a sample. The disclosure includes the following elements: (1) a lateral flow strip for microfluidic manipulation of a sample; (2) a cassette device for containing the lateral flow strip and enabling interface with a detection device; (3) a cassette handler; (4) a luminous reagent delivery device; and (5) an electromagnetic radiation detection device capable of converting chemiluminescent radiation from the lateral flow strip into an output for a user.

Techniques for colorimetric based test strip analysis and reader system are provided. In one aspect, a method of test strip analysis includes: illuminating a test strip wetted with a sample with select spectrums of light, wherein the test strip includes test pads that are configured to change color in the presence of an analyte in the sample; obtaining at least one digital image of the test strip; and analyzing color intensity from the at least one digital image against calibration curves to determine an analyte concentration in the sample with correction for one or more interference substances in the sample that affect the color intensity. A calibration method and a reader device are also provided.

Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N Na OH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

Measuring oxygen concentration and monitoring changes in oxygen concentration within an enclosed space by applying a transmembrane photoluminescent oxygen probe to the external surface of the membrane forming the enclosed space, wherein the transmembrane photoluminescent oxygen probe has an oxygen impermeable support layer carrying a spot of an oxygen-sensitive photoluminescent dye and a layer of a pressure-sensitive adhesive on a first major surface of the support layer.

A lateral flow assay format test that accepts a saliva sample that is reacted with a conjugate to indicate the level of testosterone present in the saliva sample. The concentration of testosterone is used to determine the likelihood of the saliva donors commitment to an interpersonal relationship.

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.

The invention relates to diabetic nephropathy test paper, and belongs to the technical field of medical science in vitro diagnosis. The test paper comprises water absorbing filtering paper, a glass cellulose film and an NC film, all of which are overlapped from top to bottom in sequence. The test paper is provided with a T1 testing area, a T2 testing area and a T3 referring area in the transverse direction. The part, in the T1 testing area, of the NC film is wrapped by a urine microalbumin antibody; the part, in the T2 testing area, of the NC film is wrapped by a urinary haptoglobin antibody; the part, in the T3 referring area, of the NC film is wrapped by mouse anti-human IgG. The part, in the T1 testing area, of the glass cellulose film is wrapped by urine microalbumin marked by colloidal gold; the part, in the T2 testing area, of the glass cellulose film is wrapped by urinary haptoglobin; the part, in the T3 referring area, of the glass cellulose film is wrapped by mouse anti-human IgG marked by the colloidal gold. According to the test paper, the urine microalbumin and the urinary haptoglobin serve as biological target match, the content of the urine microalbumin and the content of the urinary haptoglobin in urine can be detected, the content ratio of the urine microalbumin to the urinary haptoglobin serves as the diagnosis basis, and operation is easy, quick and accurate.