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Diabetic nephropathy test paper

A technique for detecting diabetic nephropathy and test strips, which is applied in biological testing, disease diagnosis, measuring devices, etc., can solve the problems of detection and diagnosis basis, failure, and low detection accuracy, and achieve simple operation, low missed diagnosis rate, and prevention of disease deterioration Effect

Inactive Publication Date: 2014-09-10
SHENZHEN LEADMED MEDICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing diabetic nephropathy detection test paper detects urine through colloidal gold immunochromatography, which can only detect type I diabetes or type II diabetes, and the detection accuracy is not high, so it cannot be used as an accurate detection and diagnosis basis.

Method used

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Embodiment

[0021] Such as figure 1 Diabetic nephropathy detection test paper shown, including absorbent filter paper 1, glass cellulose membrane 2, NC membrane 3, absorbent filter paper 1, glass cellulose membrane 2, NC membrane 3 are stacked sequentially from top to bottom, T1 detection area, T2 detection area and the T3 reference area are located in the middle area of ​​the test paper; the NC membrane 3 is coated with urinary microalbumin antibody 31 in the T1 detection area, coated with urinary haptoglobin antibody 32 in the T2 detection area, and coated with mouse antibody in the T3 reference area. Human IgG33; the glass cellulose membrane 2 is coated with colloidal gold-labeled urinary microalbumin 21 in the T1 detection area, colloidal gold-labeled urinary haptoglobin 22 in the T2 detection area, and coated with colloidal gold-labeled urine haptoglobin 22 in the T3 reference area Mouse anti-human IgG protein 23 labeled with colloidal gold. The urinary microalbumin antibody is a va...

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Abstract

The invention relates to diabetic nephropathy test paper, and belongs to the technical field of medical science in vitro diagnosis. The test paper comprises water absorbing filtering paper, a glass cellulose film and an NC film, all of which are overlapped from top to bottom in sequence. The test paper is provided with a T1 testing area, a T2 testing area and a T3 referring area in the transverse direction. The part, in the T1 testing area, of the NC film is wrapped by a urine microalbumin antibody; the part, in the T2 testing area, of the NC film is wrapped by a urinary haptoglobin antibody; the part, in the T3 referring area, of the NC film is wrapped by mouse anti-human IgG. The part, in the T1 testing area, of the glass cellulose film is wrapped by urine microalbumin marked by colloidal gold; the part, in the T2 testing area, of the glass cellulose film is wrapped by urinary haptoglobin; the part, in the T3 referring area, of the glass cellulose film is wrapped by mouse anti-human IgG marked by the colloidal gold. According to the test paper, the urine microalbumin and the urinary haptoglobin serve as biological target match, the content of the urine microalbumin and the content of the urinary haptoglobin in urine can be detected, the content ratio of the urine microalbumin to the urinary haptoglobin serves as the diagnosis basis, and operation is easy, quick and accurate.

Description

technical field [0001] The invention belongs to the technical field of medical in vitro diagnosis and relates to a test paper for detecting diabetic nephropathy. Background technique [0002] Diabetes is a metabolic disease characterized by high blood sugar. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. These are called diabetes complications. Among the many complications of diabetes, diabetic nephropathy caused by long-term high blood pressure and uncontrolled high blood sugar, which causes chronic renal failure or uremia in diabetic patients, is the main cause of death in patients with advanced diabetes. Therefore, for more than 100 million diabetic patients in China, the early diagnosis of diabetic nephropathy is particularly important. [0003] The traditi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/04G01N2800/042G01N33/54306G01N21/78G01N2800/347G01N2333/76G01N2333/4713G01N2021/7763G01N2021/7759G01N2021/757G01N2021/752G01N2035/00118
Inventor 岳朋周锦源盛威玮
Owner SHENZHEN LEADMED MEDICAL TECH
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