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Biosensor based on Ti3C2 two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and preparation method thereof

A technology of biosensors and metal carbides, applied in chemiluminescence/bioluminescence, chemical instruments and methods, organic compounds/hydrides/coordination complex catalysts, etc., can solve the problems of few application reports

Active Publication Date: 2018-09-21
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the Ti 3 C 2 The applications of MXenes in biosensors and biomedicine such as cancer therapy, cellular uptake, and antibacterial activity have been rarely reported

Method used

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  • Biosensor based on Ti3C2 two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and preparation method thereof
  • Biosensor based on Ti3C2 two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and preparation method thereof
  • Biosensor based on Ti3C2 two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and preparation method thereof

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Experimental program
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Effect test

preparation example Construction

[0031] The present application provides a preparation method of the above-mentioned probe, linking molecules with nanosheet Ti 3 C 2 After the MXenes are placed in water and mixed evenly, they are stirred for a period of time and centrifuged to obtain a precipitate, which can be obtained by performing amide reaction with the biorecognition molecule 1.

[0032] Preferably, the stirring time is 1-1.5 h. The rotational speed of centrifugation exceeds 10000rpm.

[0033] Preferably, the reaction system of the amide reaction is 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide sodium salt (NHS).

[0034] This application preferably has a kind of etching Ti 3 AlC 2 method, the Ti 3 AlC 2 Immerse the powder in 48±2% (mass) HF and stir at 45±2°C for 24±0.5 hours, centrifuge the powder particles and wash 5-6 times at 4500-5500rpm for 5 minutes each time, discard the supernatant liquid, dried at room temperature to obtain multilayer Ti ...

Embodiment 1

[0052] Synthesis of MXenes-aptamer1 nanoprobes

[0053] Ti 3 AlC 2 (1.0 g) powder was immersed in 15 mL of 48% (mass) HF and stirred at 45°C for 24 hours. Centrifuge and wash the powder particles at 5000rpm several times for 5 minutes each time, discard the supernatant, and dry at room temperature to obtain the layered Ti 3 C 2 T x , and store at 4°C for later use.

[0054] Layered Ti 3 C 2(0.05g) powder was immersed in 1mL DMSO and stirred at room temperature for 24 hours, centrifuged at 12000rpm for 5 times, 5 minutes each time, then discarded the supernatant and added deionized water to pulverize in a cell lyser for 2 hours, and finally , the solution was centrifuged at 3500rpm for 60 minutes, and the supernatant (that is, the nanosheet Ti 3 C 2 MXenes dispersion) and stored at 4°C for future use. For its structural characterization, see figure 2 .

[0055] Mix 200 μL (0.005 g / mL) of PEI and 3 mL of nanosheet Ti 3 C 2 MXenes were mixed, then 2 mL of deionized...

Embodiment 2

[0067] This embodiment is the same as Embodiment 1, the difference is:

[0068] Assembly of electrodes

[0069] GCE after AuNPs modification treatment: Take 6 μL of AuNPs (18nm) dispersion and drop it on the surface of the glassy carbon electrode, incubate at 37°C until dry, then soak the electrode in 400 μM EDC, 100 μM NHS, and 2 mg / mL EDA at 37°C Under incubation for 2h. At the same time during this period, 1mg mL -1 Mix 40 μL each of carboxy-terminated PNIPAM, 400 μM EDC, and 100 μM NHS, and activate at room temperature for 1 h. The glassy carbon electrode incubated in EDA was further soaked in the activated PNIPAM solution for 1 hour, and incubated for 1 hour. Then the electrode was immersed in 0.8 μM aptamer2, incubated at 37°C for 2 hours, washed and dried to obtain a biosensor electrode, which was designated as aptamer2 / PNIPAM / AuNPs / GCE.

[0070] Assembly of the sensor

[0071] Submerge aptamer2 / PNIPAM / AuNPs / GCE in different concentrations of exosomes for 1 h at 25...

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Abstract

The invention discloses a biosensor based on a Ti3C2 two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and a preparation method thereof. The biosensor based comprises a probe and a biosensor electrode, wherein the probe comprises nanosheets Ti3C2 MXenes, joint molecules and biometric molecules 1; the nanosheets Ti3C2 MXenes are connected with the joint molecules through electrostatic adsorption; the joint molecules are connected with the biometric molecules 1 through amide groups; the joint molecules contain primary or secondary amine groups; the joint molecules can be positively charged after being dissolved in water; the biometric molecules 1 are single-stranded DNA sequences 1 having carboxyl groups at the 5' ends; the single-stranded DNA sequences 1 can recognize CD63 protein on exosomes. The invention finds that Ti3C2 MXenes can improve the electrochemiluminescence of luminol for the first time, and by virtue of the property, the Ti3C2 MXenes is prepared into the probe and then the biosensor is prepared.

Description

technical field [0001] The present invention relates to the field of materials and analytical chemistry, specifically, to a novel two-dimensional nanomaterial-Ti 3 C 2 MXenes catalyzed luminol electrochemiluminescence and utilized carboxyl-terminated poly(N-isopropylacrylamide) (PNIPAM) polymer molecules to expose more active sites at a suitable temperature to construct an electrochemiluminescent biosensor to detect external secretory method. Background technique [0002] Exosomes are nanoscale extracellular vesicles (30-100 nm) released from multivesicular bodies through the endolysosomal pathway. Exosomes carry abundant cellular genetic material, including transmembrane and cytoplasmic proteins, mRNA, DNA, and microRNA, thereby serving as intercellular mediators. They play an important role. Experiments have shown that they are related to diseases, especially the onset of cancer. Exosomes are considered as biomarkers for early cancer diagnosis and are of great significa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/327G01N27/30C09K11/06
CPCC09K11/06C09K2211/1044G01N21/763G01N27/308G01N27/3278C09K11/07G01N21/76G01N33/582G01N27/4146B01J31/2295B01J2231/40B01J2531/0211B01J2531/46C09K2211/1018G01N33/5438G01N33/551G01N2021/757
Inventor 王宗花张慧欣刘洋
Owner QINGDAO UNIV
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