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A biosensor and preparation method based on three-titanium carbide two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe

一种生物传感器、金属碳化物的技术，应用在化学发光/生物发光、化学仪器和方法、有机化合物/氢化物/配位配合物催化剂等方向，能够解决应用报道很少等问题

Active Publication Date: 2020-01-03
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the Ti 3 C 2 The applications of MXenes in biosensors and biomedicine such as cancer therapy, cellular uptake, and antibacterial activity have been rarely reported

Method used

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  • A biosensor and preparation method based on three-titanium carbide two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe
  • A biosensor and preparation method based on three-titanium carbide two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe
  • A biosensor and preparation method based on three-titanium carbide two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe

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Experimental program
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Effect test

preparation example Construction

[0031] The present application provides a preparation method of the above-mentioned probe, linking molecules with nanosheet Ti 3 C 2 After the MXenes are placed in water and mixed evenly, they are stirred for a period of time and centrifuged to obtain a precipitate, which can be obtained by performing amide reaction with the biorecognition molecule 1.

[0032] Preferably, the stirring time is 1-1.5 h. The rotational speed of centrifugation exceeds 10000rpm.

[0033] Preferably, the reaction system of the amide reaction is 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide sodium salt (NHS).

[0034] This application preferably has a kind of etching Ti 3 AlC 2 method, the Ti 3 AlC 2 The powder is immersed in 48±2% (mass) HF and stirred at 45±2°C for 24±0.5 hours, the powder particles are centrifuged and washed at 4500~5500rpm for 5 minutes each time for 5~6 times, and the supernatant is discarded liquid, dried at room temperatu...

Embodiment 1

[0052] Synthesis of MXenes-aptamer1 nanoprobes

[0053] Ti 3 AlC 2 (1.0 g) powder was immersed in 15 mL of 48% (mass) HF and stirred at 45°C for 24 hours. Centrifuge and wash the powder particles at 5000rpm several times for 5 minutes each time, discard the supernatant, and dry at room temperature to obtain the layered Ti 3 C 2 T x , and store at 4°C for later use.

[0054] Layered Ti 3 C 2(0.05g) powder was immersed in 1mL DMSO and stirred at room temperature for 24 hours, centrifuged at 12000rpm for 5 times, 5 minutes each time, then discarded the supernatant and added deionized water to pulverize in a cell lyser for 2 hours, and finally , the solution was centrifuged at 3500rpm for 60 minutes, and the supernatant (that is, the nanosheet Ti 3 C 2 MXenes dispersion) and stored at 4°C for future use. For its structural characterization, see figure 2 .

[0055] Mix 200 μL (0.005 g / mL) of PEI and 3 mL of nanosheet Ti 3 C 2 MXenes were mixed, then 2 mL of deioniz...

Embodiment 2

[0067] This embodiment is the same as Embodiment 1, the difference is:

[0068] Assembly of electrodes

[0069] GCE after AuNPs modification treatment: Take 6 μL of AuNPs (18nm) dispersion and drop it on the surface of the glassy carbon electrode, incubate at 37°C until dry, then soak the electrode in 400 μM EDC, 100 μM NHS, and 2 mg / mL EDA at 37°C Under incubation for 2h. At the same time during this period, 1mg mL -1 Carboxyl-terminated PNIPAM, 400 μM EDC, and 100 μM NHS were mixed in 40 μL each, and activated at room temperature for 1 h. Continue to soak the glassy carbon electrode incubated in EDA in the activated PNIPAM solution for 1 hour, and incubate for 1 hour. Then the electrode was immersed in 0.8 μM aptamer2, incubated at 37°C for 2 hours, washed and dried to obtain a biosensor electrode, which was designated as aptamer2 / PNIPAM / AuNPs / GCE.

[0070] Assembly of the sensor

[0071] Submerge aptamer2 / PNIPAM / AuNPs / GCE in different concentrations of exosomes for 1 h...

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Abstract

The invention discloses a biosensor based on three-titanium carbide two-dimensional metal carbide catalyzed luminol electrochemiluminescence probe and a preparation method thereof. The biosensor includes a probe and a biosensor electrode, and the probe includes a nanosheet Ti 3 C 2 MXenes, linker molecules and biorecognition molecules 1, the nanosheet Ti 3 C 2 MXenes are connected to the linker molecule through electrostatic adsorption, the linker molecule is connected to the biorecognition molecule 1 through an amide group, the linker molecule contains a primary or secondary amine group, and the linker molecule can carry Positively charged, the biorecognition molecule 1 is a single-stranded DNA sequence 1 with a carboxyl group at the 5' end, and the single-stranded DNA sequence 1 can recognize the CD63 protein on the exosome. The invention discovered Ti for the first time 3 C 2 MXenes can improve the electrochemiluminescence of luminol, and use this property to prepare it into probes, and then prepare biosensors.

Description

technical field [0001] The present invention relates to the field of materials and analytical chemistry, specifically, to a novel two-dimensional nanomaterial-Ti 3 C 2 MXenes catalyzed luminol electrochemiluminescence and utilized carboxyl-terminated poly(N-isopropylacrylamide) (PNIPAM) polymer molecules to expose more active sites at a suitable temperature, thereby constructing an electrochemiluminescence biosensor to detect external secretory method. Background technique [0002] Exosomes are nanoscale extracellular vesicles (30-100 nm) released from multivesicular bodies through the endolysosomal pathway. Exosomes carry abundant cellular genetic material, including transmembrane and cytoplasmic proteins, mRNA, DNA, and microRNA, thereby serving as intercellular mediators. They play an important role. Experiments have shown that they are related to diseases, especially the onset of cancer. Exosomes are considered as biomarkers for early cancer diagnosis and are of great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/327G01N27/30C09K11/06
CPCC09K11/06C09K2211/1044G01N21/763G01N27/308G01N27/3278C09K11/07G01N21/76G01N33/582G01N27/4146B01J31/2295B01J2231/40B01J2531/0211B01J2531/46C09K2211/1018G01N33/5438G01N33/551G01N2021/757
Inventor 王宗花张慧欣刘洋
Owner QINGDAO UNIV
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