Testing device for identifying antigens and antibodies in biofluids

a biofluid and antigen technology, applied in the field of identification of antigens and antibodies within biofluids, can solve the problem of no convenient low cost disposable tests available for “on the spo

Inactive Publication Date: 2012-12-20
MONASH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surprisingly, and in spite of its vital importance, there are no convenient

Method used

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  • Testing device for identifying antigens and antibodies in biofluids
  • Testing device for identifying antigens and antibodies in biofluids
  • Testing device for identifying antigens and antibodies in biofluids

Examples

Experimental program
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example 1

Sequential Agglutination / Coagulation of Blood Followed by Wicking on Paper: B+ (Two Step Process) (See FIG. 1)

[0044]Antibody A and B (Epiclone™ Anti-A, Anti-B, and Anti-D; CSL, Australia) solutions were used. Anti-A and Anti-B come as blue and yellow colour reagents, respectively. ‘B+’ blood was used in this study. The blood sample was supplied into plastic vials with anti-coagulant. ‘B+’ blood was separately mixed with pure Anti-A and Anti-B (as received) to prepare 100 μL solution. Paper strips (70 mm×2 mm) were made from Whatman#4 filter paper on which 2 mm unit marks were printed. The paper strips were soaked into phosphate buffer saline (PBS). Excess PBS was removed from the paper strips using standard blotting papers (Drink Coster Blotting, 280 GSM). The paper strips were then placed on Reflex Paper (80 GSM). 20 μL of every mixed solution was dispensed at the centre of paper strip using a calibrated micro-pipette. Pictures were taken after 4 minutes wicking.

[0045]It can be see...

example 2

Sequential Agglutination / Coagulation of Blood Followed by Wicking on Paper: O+ (Two Step Process) (See FIG. 2)

[0052]Antibody A and B (Epiclone™ Anti-A, Anti-B and Anti-D; CSL, Australia) solutions were used. Anti-A and Anti-B come as blue and yellow colour reagents, respectively. ‘O+’ blood was used in this study. The blood sample was supplied into plastic vials with anti-coagulant. ‘O+’ blood was separately mixed with Anti-A and Anti-B to prepare 100 μL solution. Paper strips (70 mm×2 mm) were made from Whatman#4 filter paper on which 2 mm unit marks were printed. The paper strips were soaked into phosphate buffer saline (PBS). Excess PBS was removed from the paper strips using standard blotting papers (Drink Coster Blotting, 280 GSM). The paper strips were then placed on Reflex Paper (80 GSM). 20 μL of every mixed solution was dispensed at the centre of paper strip using a calibrated micro-pipette. Pictures were taken after 4 minutes wicking.

[0053]It can be seen that:

[0054]O+ bloo...

example 3

Simultaneous Agglutination / Coagulation of Blood Followed by Wicking on Paper: Effect of Antigen Concentration (One Step Process) (See FIG. 3)

[0060]In another embodiment of the invention, the paper is first treated with specific antibodies, dried or conditioned before been exposed to a sample of pure blood. This example provides a single step treatment in which the only requirement is to deposit a drop of blood on the paper. This example also illustrates the effect of diluting the antibody solution on the wicking and separation performance of blood on paper. Antibody dilution affects the ratio blood (with its antigen) antibody.

[0061]Antibody A and B (Epiclone™ Anti-A and Anti-B; CSL, Australia) solutions were used. Anti-A and Anti-B come as blue and yellow colour reagents, respectively. “AB+” and ‘B+’ blood were used in this study. The blood sample was supplied into plastic vials with anti-coagulant. Paper strips (70 mm×2 mm) were made from Whatman#4 filter paper on which 2 mm unit m...

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Abstract

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.

Description

FIELD OF THE INVENTION[0001]The present invention is directed to the identification of antigens and antibodies within a biofluid. While the invention will be described with specific reference to its use in determining a person's blood type, it is to be appreciated that other applications of the invention are also envisaged.BACKGROUND TO THE INVENTION[0002]Blood is essential for sustaining living tissue, with the most important roles of supplying oxygen and other soluble nutrients, immune protection and metabolic turnover. While it is a tissue in its own right, blood in a chemical sense can be considered as a stable, highly packed colloid suspension made of red blood cells (erythrocytes, 4-6 million / mL, 6-8 μm), white cells (leukocytes, 4000-6000 / mL, 10-21 μm), platelets (150,000-400,000 / mL, 2-5 μm) carried within a fluid solution (serum) containing a host of biomolecules (eg albumins, fatty acids, hormones), metabolites and electrolytes. A subset of these biomolecules, such as the b...

Claims

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Application Information

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IPC IPC(8): C12M1/34G01N21/82
CPCG01N30/90G01N33/558G01N21/82G01N33/80G01N2021/752G01N2021/757G01N2021/825G01N33/54389
Inventor GARNIER, GILSHEN, WEIKHAN, MOHIDUS SAMADLI, XUTHOUAS, GEORGE
Owner MONASH UNIV
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