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Immune detection kit

An immunoassay and kit technology, applied in the field of immunochromatography, can solve the problems that the test line 12T' cannot be effectively suppressed, the maximum can only reach 0.5ppb, and the manufacturing cost is high.

Inactive Publication Date: 2017-03-22
REGA BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] (1) The manufacturing cost of the bonding pad 13' made of microporous material is relatively high; in addition, the bonding pad 13' made of microporous material needs to be stored at a low temperature of 4°C, and its storage period is only about 1 year at most
[0014] (2) The commercially available (competitive) lateral flow immunoassay test paper 1' cannot effectively suppress the color development of the test line 12T', so it is necessary to judge the chromaticity of the control line 12C' and the test line 12T' by colorimetry ; Among them, the change of color can be judged by naked eyes (qualitative method), and the change of brightness can also be automatically detected by an instrument (quantitative method); however, the sensitivity judged by colorimetry can only reach 0.5ppb at the highest

Method used

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Examples

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Effect test

no. 1 example

[0061] The first embodiment of the immunoassay kit 1 is designed based on the realization of a competitive lateral flow immunoassay. Please refer to figure 2 , in the first embodiment of the immunoassay kit 1, the binding pad 111 of the release reagent sheet 11 is provided with a first antibody bound to a marker, and the first antibody is the rabbit's anti-aflatoxin antibody C ( or mouse antibodies against aflatoxin). In addition, it must be noted that the present invention does not particularly limit the type of markers, so markers can be colloidal gold (colloidal gold), colloidal selenium (colloidal selenium), latex (latex), nano silver, or nano Carbon (nano carbon). A first antibody is provided relative to the binding pad 111, and the control line 12C is formed by a second antibody, and the second antibody is goat anti-rabbit immunoglobulin G (Immunoglobulin G, IgG), or rabbit anti-mouse immunoglobulin G, or goat anti-mouse IgG. In addition, the test line 12T is formed...

no. 2 example

[0083] See figure 2 , the second embodiment of the immunoassay kit 1 is designed based on the implementation of a sandwich-type lateral flow immunoassay. In the second embodiment of the immunoassay kit 1 , the binding pad 111 of the release reagent sheet 11 is provided with a first antibody bound to a marker, and the first antibody is an antibody Ab2 against bran (gluten protein). With respect to the first antibody provided on the binding pad 111 , the control line 12C is formed by the second antibody, and the test line 12T is formed by the third antibody. Here, the second antibody is goat anti-rabbit immunoglobulin G (Immunoglobulin G, IgG), rabbit anti-mouse immunoglobulin G, or goat anti-mouse immunoglobulin G; and, the third antibody is Antibody Abl against bran (gluten protein). Here, it must be particularly noted that the above-mentioned antibodies are not particularly limited to monoclonal antibodies or polyclonal antibodies.

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Abstract

The present invention provides an immune detection kit, which is mainly composed of a release reagent plate and a reaction reagent plate. The immune detection kit is different from lateral flow immunoassay test paper in the prior art, when the immune detection kit is used for competitive or sandwich type lateral flow immunoassay of a sample to be tested, first, the release reagent plate must be placed into a sample liquid for release into the sample liquid and further mutual combination with a target detection matter in the sample liquid of a marker-bonded antibody (or drug) which is carried by the release reagent plate; and finally the reaction reagent plate is placed into the sample liquid, so that the sample liquid and an antibody which is carried by a test line on the reaction reagent plate compete or combine with each other, and whether a test result is positive or negative can be judged by whether the test line shows color or not.

Description

technical field [0001] The invention relates to the technical field of immunochromatography, in particular to an immunoassay kit composed of a release reagent sheet, a reaction reagent sheet and a buffer diluent. Background technique [0002] Immunochromatographic test (immunochromatographic test) is a detection method that has received great attention in recent years. The detection test paper has the advantages of simple structure and low cost; more importantly, the highest sensitivity of immunochromatographic test can reach 10-12g / ml (ppm). At present, immunochromatographic detection has been widely used in the following aspects: [0003] (1) Clinical applications: detection of allergies, drugs, infectious diseases, endocrine diseases, tumor markers, etc.; [0004] (2) Agricultural applications: such as detection of food safety and crop diseases; [0005] (3) Environmental applications: detection of biological pollution, environmental pollution, etc.; [0006] (4) Vete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
CPCG01N33/54388G01N21/8483G01N2021/8488G01N21/78G01N2021/7759G01N2021/757G01N2021/752
Inventor 林淑瑜
Owner REGA BIOTECH
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