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Detection method and kit for simultaneously detecting 19 elements in human body

A technology for detecting human body and detection method, which is applied in the field of analysis and detection, and can solve problems such as pre-treatment of hair samples being easily contaminated, troublesome and complicated pre-treatment of blood samples, etc.

Inactive Publication Date: 2018-06-29
GUANGZHOU BIOHOP TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pre-treatment of blood samples is cumbersome and complicated; the hair samples are easily contaminated and the pre-treatment is complicated; the sampling of 24-hour urine samples is cumbersome and requires 24-hour urine collection and transportation is troublesome

Method used

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  • Detection method and kit for simultaneously detecting 19 elements in human body
  • Detection method and kit for simultaneously detecting 19 elements in human body
  • Detection method and kit for simultaneously detecting 19 elements in human body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The detection method of nineteen elements in this embodiment includes the following steps:

[0061] 1. The main reagents and instruments

[0062] American Agilent Technologies ICP-MS (7900), IKA VOTEX3 vortex oscillator, German Eppendorf pipette and centrifuge tube, Vitlab volumetric flask and narrow-mouth flask, AΜW220D electronic balance.

[0063] ΜP-S grade isopropanol, the analysis water is the first grade water specified in GB / T6682, the resistivity is ≥ 18.2MΩ.cm, the LC-MS / MS grade water (Fisher Chemical W6-4), the pure nitric acid of superior grade, 1000mg / L gold standard solution, Triton X-100Bioxtra from SigmaAldrich, argon gas, liquid argon> 99.999%.

[0064] Second, the configuration of the solution

[0065] 2.1 Diluent (2mg / L gold standard, 0.1% Triton X-100, 0.1% nitric acid)

[0066] Take 200mL Fisher deionized water in a 250mL volumetric flask, add 385μL of 65% concentrated nitric acid, 500μL of 1000mg / L gold standard solution, 250μL of Triton X-100 solution, dil...

Embodiment 2

[0119] Example 2 Methodological verification

[0120] In this example, the method for detecting the nineteen elements in Example 1 was verified.

[0121] 1. Precision

[0122] Intra-assay precision: take low, medium, and high levels of quality control samples for intra-assay precision experiments; 20 samples of each concentration level are processed in parallel, and each sample is injected once; that is, 20 sets of data for each concentration level, Calculate the average and relative standard deviation (RSD) of the test results.

[0123] Inter-assay precision: take pre-prepared quality control products at three levels of low, medium, and high, and conduct inter-assay precision experiments; do 4 samples at each level in parallel and measure continuously for 5 days; that is, each concentration level is 20 sets of data, calculate the average and relative standard deviation (RSD) of the test results.

[0124] Precision verification results: The precision experimental verification results ...

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Abstract

The invention relates to a detection method and a kit for simultaneously detecting 19 elements in a human body. The 19 elements are zinc, strontium, selenium, lithium, aluminum, molybdenum, arsenic, iron, barium, copper, lead, mercury, nickel, antimony, manganese, cadmium, thallium, chromium and cobalt respectively. The detection method comprises the following steps of (1), preparing diluent whichis a 0.1wt% to 2wt% nitric acid aqueous solution containing 1.5mg / L to 2.5mg / L of golden standard and 0.05wt% to 0.2wt% of triton 100, preparing a standard solution which is prepared by adding standard products of the 19 elements into the diluent, and preparing an internal standard solution which is prepared by adding standard products of internal standard elements into the diluent; (2), collecting urine at any time point, wherein the urine is diluted through the diluent, so that a to-be-detected sample is obtained; (3), detecting by adopting inductively coupled plasma-mass spectrometry (ICP-MS). The detection method can be used for simultaneously detecting the 19 elements in the human body; a sampling method is simple; a sample is easily stored; the pretreatment is simple; the sensitivity is high; the specificity is high, and the detection flux is high.

Description

Technical field [0001] The present invention relates to the technical field of analysis and detection, in particular to a method and a kit for simultaneously detecting nineteen elements of the human body by using plasma mass spectrometry (ICP-MS). Background technique [0002] The human body is an organism composed of various chemical elements. According to the physiological role of each element in the human body, it can be divided into nutrient elements and toxic elements. Nutritional elements refer to elements that have special physiological functions and are beneficial to maintaining normal life activities of the human body, such as calcium, phosphorus, magnesium, potassium, sodium, iron, zinc, copper, selenium, etc. Toxic elements refer to elements that can cause harmful effects on the human body if they exist in a small amount. Common heavy metals such as arsenic, cadmium, lead, and mercury. [0003] The imbalance of elements (lack of nutrient elements or too high toxic elem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/38
CPCG01N1/38G01N27/62
Inventor 何翠红李玉冬梁田卢章准陈栢健熊晓明
Owner GUANGZHOU BIOHOP TECH INC
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