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A single cell sampling device and sampling method based on a porous metal enrichment probe

A technology of porous metal and sampling device, which is applied to biological material sampling methods, biochemical equipment and methods, biochemical instruments, etc. It can solve the problems of high cost, difficult cleaning of probes, cumbersome process, etc., and achieve low cost and simple operation Convenient and repeatable effect

Active Publication Date: 2019-03-05
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problems of the capillary probe-based single-cell sampling device in the prior art, the cost is high, the probe is not easy to clean, and the single-cell sampling method based on the capillary probe is cumbersome. Single-cell sampling device and sampling method for enriching probes

Method used

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  • A single cell sampling device and sampling method based on a porous metal enrichment probe
  • A single cell sampling device and sampling method based on a porous metal enrichment probe
  • A single cell sampling device and sampling method based on a porous metal enrichment probe

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preparation example Construction

[0046] Taking the preparation of porous copper-enriched probes as an example,

[0047] The preparation process of the probe described above. First take the copper alloy wire, in the copper alloy wire, the copper element contains 20-60%, and the active metals are active metals such as zinc, aluminum, magnesium, manganese, silver, etc., which can be one or a variety of mixtures, and the diameter A copper alloy wire of about 30-3000 μm is cut into small sections of several centimeters, and a tip with a diameter of 1-20 μm is cut at one end of each small piece of wire. The cut metal wire is corroded in a strong acid solution, the active metal will be selectively decomposed, and the remaining copper elements can spontaneously form a three-dimensional continuous porous structure, which is then rinsed with ultrapure water and / or methanol and then vacuum-dried to obtain porous copper. probe.

[0048] The sampling method of the above-mentioned single cell sampling device based on the...

Embodiment 1

[0058] A single-cell sampling device based on a porous metal enrichment probe, consisting of a linear controller 1, a porous metal enrichment probe 2 and a microscope 3; the linear controller 1 consists of a base 11, a pole 12, a clamping device 13, and a differential head 14 and a fixed base 15, the pole 12 is fixed on the base 11, one end of the clamping device 13 is fixed on the pole 12, and the other end clamps the mounting sleeve of the differential head 14, the fixed base 15 and the measuring rod of the differential head 14 connection, the clamping device 12 is used to adjust the angle between the porous metal enrichment probe 2 and the glass slide 31, and the differential head 14 is used to insert the porous metal enrichment probe 2 into the depth and time of the target cell;

[0059] The porous metal enrichment probe 2 is a three-dimensional continuous porous structure with one end as a tip and the other end fixed on a fixed seat. The porous metal enrichment probe moves...

Embodiment 2

[0064] The method for sampling the onion inner epidermis using the single cell sampling device of embodiment 1:

[0065] Step 1. Adjust the clamping device 13 so that the porous metal enrichment probe 2 and the slide glass 31 form an angle of 30 degrees;

[0066] Step 2, place the onion inner epidermis slice on the glass slide 31, observe the onion inner epidermal cells through the objective lens 32 of the microscope 3, and select a cell as the target cell;

[0067] Step 3: Rotate the differential head 14 to insert the porous metal enrichment probe 2 into the target cell, stay for 120 seconds, rotate the differential head 14 again to pull out the porous metal enrichment probe 2, and complete the single cell sampling;

[0068] Wash the porous metal-enriched probe 2 tip enriched with samples in 1 μL of 10 mg / ml DHB solution, then spot the washing solution on the plate and analyze it with MALDI-TOF-MS, the results are as follows Figure 5 shown. from Figure 5 It can be seen t...

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Abstract

The invention discloses a unicellular sampling device and method based on a porous metal enrichment probe, and belongs to the technical field of unicellular sampling. The problem that existing unicellular sampling devices and methods based on capillary tube probes are high in cost, poor in repeatability and complicated in process is solved. The sampling device comprises a linear controller, the porous metal enrichment probe and a microscope, wherein the linear controller is composed of a base, a supporting rod, a clamping device, a micrometer head and a fixing base, the porous metal enrichment probe has a three-dimensional continuous porous structure, one end of the porous metal enrichment probe is a tip end, the other end of the porous metal enrichment probe is fixed to the fixing base, the porous metal enrichment probe moves in the axis direction of the micrometer head along with a measuring rod of the micrometer head, and the microscope is used for observing cells in tissue to be sampled. The unicellular sampling device is easy and convenient to operate, good in repeatability, low in cost, high in efficiency and capable of achieving unicellular sampling of animal and plant tissue, then, rapid analysis of unicellular metabolins is achieved, and differences between the metabolins among different cells are found.

Description

technical field [0001] The invention relates to a single cell sampling device and a sampling method based on a porous metal enrichment probe, belonging to the technical field of single cell sampling. Background technique [0002] Single-cell analysis technology is a technology that detects trace metabolites in a single cell through a method with high sensitivity and high specificity. For fresh animal and plant tissues, different cells in the same tissue often have similar genes and growth environments. However, in recent years, scientists have discovered that different cells in the same tissue may have significantly different stress responses to the same signal, resulting in significant differences in intracellular metabolites. Such changes at the single-cell level are associated with various biological phenomena such as growth, differentiation, heterogeneity, stress response, aging, apoptosis, and carcinogenesis. Therefore, studying the changes of metabolite types and con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/26
CPCC12M33/04
Inventor 崔勐付强唐君刘欢
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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