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Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage

A rhizosphere soil and cultivation device technology, which is applied to the field of root box cultivation devices for rhizosphere soil sampling in the seedling stage of forest trees, can solve problems such as wide distribution, lack of research methods and means, and root tissue residues, and achieves expansion of space range and simplification. Sampling method, effect of increasing accuracy

Inactive Publication Date: 2011-08-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of forestry, due to the thick and widely distributed root system of forest trees, it is difficult to control, and the application of traditional root boxes in agriculture is limited to a certain extent. At present, the extensive shake-off collection method is still mostly used for rhizosphere soil sampling (Zhang Jinlong et al. 2003)
This method is affected by conditions such as soil type, humidity, and plant root system, and its sampling range is difficult to control. There are great defects in sampling accuracy, and the sampling time is long, and it is easy to cause some root tissue residues. , thus affecting the analysis and determination of rhizosphere soil nutrient transformation and microbial characteristics, resulting in a lack of accuracy in research results
In addition, the distance gradient effect of the rhizosphere process cannot be studied because there is no way to distinguish different layers of soil from the root
Therefore, the lack of suitable research methods and means has become a bottleneck limiting the research on nutrient transformation and microbial characteristics of forestry rhizosphere soil

Method used

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  • Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage
  • Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage
  • Rhizobox culture device applicable for sampling rhizosphere in forest seedling stage

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Embodiment 1

[0027] A kind of root box culture device that is applicable to the root box soil sampling of tree seedling stage rhizosphere soil of the present invention comprises a detachable rectangular box container, and this box container is made of figure 1 The seedling growth room 1 on the right side and the rhizosphere soil sampling room 2 on the left side are composed.

[0028] Seedling growth chamber 1 is surrounded by 2 side panels 11, 1 side panel 12 and 1 bottom panel 13, and the panels all adopt 3mm thick opaque black plexiglass, 2 side panels 11 are parallel to each other, side panels 11 The height of the side plate 12 is equal, the lateral width of the side plate 11 and the bottom plate 13 are equal, and the longitudinal width of the side plate 12 and the bottom plate 13 is equal. Several drainage holes 14 are evenly arranged on the bottom plate 13, the diameter of the drainage holes 14 is 10mm, and the hole spacing is preferably 50-80mm. The side panels 11, the side panels ...

Embodiment 2

[0038] A root box culture device suitable for sampling rhizosphere soil at the seedling stage of forest trees, comprising a seedling growth chamber 1 and a rhizosphere soil sampling chamber 2 with drainage holes at the bottom, the two chambers are connected through a connecting part 3 after being flexibly connected, and the connecting part The façade where 3 is located and the drainage holes are covered with isolation nets, and in the rhizosphere soil sampling chamber 2, at least 2 interlayers 5 are arranged on the façade near the connection part 3 . The isolation net adopts a polyamide microporous membrane with a pore diameter of 30 μm and a thickness of 90 μm. The interlayer 5 is made up of an interlayer frame 51 and a separation film 52, the size of the interlayer frame is as the criterion for just embedding the rhizosphere soil sampling chamber 2, the thickness of the interlayer body is set according to the sampling requirements, and the separation film 52 is covered on the...

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Abstract

The invention discloses a rhizobox culture device applicable for sampling rhizosphere in a forest seedling stage. The device a seedling growing chamber and a rhizosphere sampling chamber, wherein water-draining holes are arranged on the bottoms of both the seedling growing chamber and the rhizosphere sampling chamber; the two chamber are movably connected through a connecting part and then communicated with each other; isolating meshes are covered on the vertical plane, in which the connecting part is positioned, and on the water-draining holes respectively; and in the rhizosphere sampling chamber, an isolating layer for sampling soil at the positions with different distances from the root is arranged near the vertical plane of the connecting part. The device is suitable for the forest seedling stage with relatively thick root system and can sample microzone soil at the positions with different distances from the root conveniently, effectively and precisely.

Description

[0001] technical field [0002] The invention relates to a root box culture device for sampling rhizosphere micro-domain soil, in particular to a root box culture device suitable for sampling rhizosphere soil at the seedling stage of forest trees. Background technique [0003] The rhizosphere (rhizosphere) has a very special and important position in the study of soil nutrient transformation, availability and its relationship with the diversity and activity of microbial communities. The rhizosphere is the most active soil microregion affected by plant living roots. The root system changes the chemical, physical and biological properties of the rhizosphere soil through uptake and secretion, which directly affects the availability of nutrients in the soil to plants. A series of research results have shown that rhizosphere processes such as rhizosphere settlement and fine root turnover are often accompanied by the enhancement of rhizosphere microbial activity, organic matter ...

Claims

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Application Information

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IPC IPC(8): A01G9/02G01N1/04
Inventor 田野刘东方升佐
Owner NANJING FORESTRY UNIV
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