Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology

A chemiluminescence immunoassay and detection reagent technology, applied in the field of immunoassay, can solve the problems of inability to meet clinical requirements, inconvenient operation and use, narrow detection range, etc., and achieve the effects of fast detection efficiency, convenient automation and simple operation.

Active Publication Date: 2014-07-30
DIRUI MEDICAL TECH CO LTD
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Problems solved by technology

Due to the pollution of radioactive elements to the environment and its short half-life, etc., the operation and use are inconvenient, which limits the applic

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  • Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology
  • Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology
  • Testosterone detection reagent based on microparticle chemiluminescence immunoassay technology

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Embodiment Construction

[0015] The present invention provides a testosterone detection reagent based on microparticle chemiluminescence immunoassay technology. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention is further described below in detail. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0016] The invention provides a testosterone detection reagent based on microparticle chemiluminescence immunoassay technology, which comprises the following components according to the composition: 0.002%-0.01% paramagnetic microspheres, 0.4μg / ml-1.0μg / ml acridine ester Labeled testosterone antibody, compound testosterone releasing agent, luminescent solution A and luminescent solution B, 0ng / dl-1500ng / dl testosterone calibrator and a certain concentration of washing solution.

[0017] More specifically, the testosterone re...

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Abstract

The invention discloses a testosterone detection reagent based on a microparticle chemiluminescence immunoassay technology; the testosterone detection reagent comprises the following components: 0.002%-0.01% of paramagnetic microspheres, 0.4 mug/ml-1 mug/ml of acridinium ester labeled testosterone antibodies, a composite testosterone release agent, a luminescence liquid A, a luminescence liquid B, 0ng/dl-1500ng/dl of a testosterone calibrator and a cleaning fluid with a certain concentration; and the testosterone release agent is a mixture of dihydrotestosterone, danazol and heparin sodium. The testosterone detection reagent has the advantages of simple operation, high sensitivity, fast detection efficiency, low cost, easy automation and the like, and by combination of the selected composite release agent and different binding globulin, combined-state testosterone in serum can be fully released, so that the content detection of total testosterone is more accurate.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a testosterone detection reagent based on microparticle chemiluminescence immunoassay technology. Background technique [0002] Testosterone is a steroid hormone that is widely present in humans and animals. It can accurately detect its content in the body and judge its reproductive performance, normal physiological index and pathological concentration for disease diagnosis and treatment, family planning, and animal reproduction. It plays an important role in breeding and so on. Testosterone in men is produced by Leydig cells, and the testosterone content in men's serum is the most important parameter for confirming suspected testicular dysfunction, proving androgen deficiency and detecting testosterone replacement therapy. In women, testosterone is mainly synthesized in the ovaries, but also in the adrenal cortex, and the testosterone content in the female serum is used to ...

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Application Information

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IPC IPC(8): G01N33/74G01N21/76
CPCG01N21/763G01N33/54326G01N33/743G01N2446/86
Inventor 孙成艳顾小丰阚洪晶高威王香琪王皓
Owner DIRUI MEDICAL TECH CO LTD
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