87results about How to "Not easy to dissociate" patented technology

The invention relates to preparation and application of a reducing sensitive nano-micelle, belonging to preparation and application of an amphiphilic block polymer. A hydrophilic section and a hydrophobic section of the amphiphilic block polymer are connected with each other by a reducing responsive sulfur-sulfur bond; the nano-micelle is formed by self-assembling; the reducing sensitive nano-micelle consists of a shell and an inner core, wherein the shell is a hydrophilic polymer, and the inner core is a hydrophobic polymer; the hydrophilic section of the amphiphilic block polymer is polyphosphate, the hydrophobic section of the amphiphilic block polymer is poly(benzyl glutamate), and the hydrophilic section and the hydrophobic section of the amphiphilic block polymer are connected with each other by the reducing responsive sulfur-sulfur bond. The reducing sensitive nano-micelle has the advantages that the drug-loaded nano-micelle is difficult to dissociate outside the cells or in the blood, so that the drug encapsulated in the nano-micelle is enabled to be stable; once the nano-micelle enters the cancer cell, the sulfur-sulfur bond can be disconnected by glutathione which is a reducing matter in the cell, so that the nano-micelle is rapidly dissociated, the anti-cancer drug encapsulated in the nano-micelle can be rapidly and effectively released out, and an effective treatment effect is achieved; the reducing sensitive nano-micelle overcomes the defects that the drug is easy to leak in vivo, low in transport efficiency, slow in intracellular release speed, and the like.

The invention discloses a protein antimicrobial catheter. A non-diffusivity nanoscale smooth coating is arranged on the surface of the material of the catheter body, the main ingredient of the coating is natural protein harmless to the human body, the thickness of the coating is 10 nm to 500 nm, and the RMS roughness of the coating is not larger than 100 nm. The invention further discloses a manufacturing method of the protein antimicrobial catheter. The method includes the main steps that the material of the catheter body is pre-processed, protein glue solution is prepared, the surface of the material of the catheter body is coated with the protein glue solution one time or multiple times, and finally the protein glue solution adhering to the surface of the material of the catheter body is cured with the method of natural curing or microwave curing or hot curing or ultraviolet curing or cross-linking curing or the like. The catheter manufactured with the method is excellent in bacteria resistance and antibacterial effects, economical burdens and body pains caused when a catheter is frequently replaced for a patient can be reduced, the catheter is good in stability and low in virulence in body, some discomfort symptoms will not be caused to the patient, and the catheter is safe in clinical use.

The invention discloses a hydrophilic polymer the side chain of which is modified by lipoic acid and preparation and application thereof. The hydrophilic polymer the side chain of which is modified bylipoic acid is an amphipathic polymer; the main chain of the amphipathic polymer is an hydrophilic polymer, and the side chain is a lipoic acid radical; the lipoic acid radical and a hydroxide radical or an amino group in the hydrophilic polymer are condensed to form an ester bond or an acidamide bond; crosslinking can be carried out by nano particles formed by the self assembly of the amphipathic polymer to obtain stable crosslinking polymer nano particles with sensitive reduction, so that the nano particles are not easy to dissociate both outside cells and in blood, thereby ensuring the stability of drugs encapsulated by the nano particles; the nano particles rapidly relieve crosslinking to dissociate once entering tumor cells, and then the drugs rapidly release to generate the high-efficiency therapeutic effect. The deficiencies that the drugs are easy to leak, the carrying efficiency is low, the release speed in the cells is slow and the like are solved.

The invention discloses a preparation method and use of a thioctic acid-modified polyethylene glycol-polyaminoacid amphiphilic triblock copolymer. A hydrophilic chain of the amphipathic triblock polymer is polyethylene glycol, a middle hydrophobic chain segment of the amphipathic triblock polymer is poly(benzyl glutamate), a tail hydrophobic chain segment of the amphipathic triblock polymer is polyphenylalanine and the side chain of the middle chain segment is modified through thioctic acid. The amphipathic triblock polymer is self-assembled in water to form a polymer nanometer micelle with polyethylene glycol as an outer crown, thioctic acid-modified poly(benzyl glutamate) as a middle shell and polyphenylalanine as an inner core. Through crosslinking of the nanometer micelle, the stable reduction-sensitive shell crosslinked nanometer micelle is obtained so that the nanometer micelle is not easily dissociated in vitro and in blood and thus stability of a nanometer micelle-coated drug is guaranteed. When the nanometer micelle enters a tumor cell, the nanometer micelle can be fast crosslinked and dissociated so that the drug can be fast released and produces high treatment effects. The amphipathic triblock polymer solves the problems of drug leakage in vivo, low conveying efficiency and slow release in cells.

The invention relates to a method for separating and culturing human adipose-derived stem cells. The method comprises the following steps: (1) digesting an adipose suction substance by adopting a mixed digestive enzyme solution which consists of collagenase I, collagenase II and ACCUTASE, neutralizing the digestive enzyme after digesting is ended, centrifuging, filtering, and then further removinghybrid cells by using a lymphocyte separating liquid; (2) culturing adipose-derived stem cells by adopting a serum-free culture medium. According to the method for separating and culturing the humanadipose-derived stem cells, on one hand, cells can be dissociated from an adipose tissue quickly and effectively, the yield of the stem cells is improved, the cell separating time is shortened, and the activity of the cells is kept to the greatest extent; on the other hand, the culture medium is clear in components, does not contain any exogenous serum component, can remarkably improve the wall adhering capacity and the multiplication capacity of the adipose-derived stem cells, is beneficial to in-vitro amplification and stemness maintenance of the adipose-derived stem cells, and has a good application prospect.

The invention discloses a preparation method of reduction self-assembled protein-coated magnetic microspheres. According to the preparation method, mercaptoethanol, dithiothreitol, or 3-mercapto-1,2-propanediol are taken as reductive agents; reduction reaction is adopted to break the disulfide bonds of protein, and expose the sulfydryl and hydrophobic regions of protein, self-assembling with magnetic nano particles via sulfydryl coordination and hydrophobic interaction is realized so as to obtain protein-coated magnetic microspheres of a core-shell structure. The particle size of the protein-coated magnetic microspheres is convenient to adjust; stability is high; water solubility is high; aggregation is not easily caused; nonspecific adsorption is low; excellent biocompatibility is achieved; no special equipment is needed; consumed time is short; operation is convenient; production cost is low; preparation conditions are mild; direct fixing of specific antibodies in preparation process is realized; the activity of the protein-coated magnetic microspheres coupled with antibodies is high; and the obtained reduction self-assembled protein-coated magnetic microspheres can be used in magnetic immunity chromatography preferably.

The invention provides a preparation method of a high-efficiency leaching and adsorbing material for Cd contaminated soil and wastes and also discloses the high-efficiency leaching and adsorbing material prepared by the preparation method. The material has quicker and higher adsorption capacity for heavy metal Cd; besides, the material is low in price and easily-obtained and facilitates wide application of a leaching remediation method for heavy metal contaminated soil; meanwhile, sulfydryl (-HS) in sulfhydryl modified attapulgite clay serving as an adsorbing material can generate a stronger complexing action with heavy metal ions, and heavy metal is not easy to dissolute, so that the sulfhydryl modified attapulgite clay has important significance on repeated leaching and adsorbing; in addition, a pH value, obtained by measuring, of the sulfhydryl modified attapulgite clay is neutral and is relatively-stable, so the sulfhydryl modified attapulgite clay has no influence on leacheate or soil and better facilitates quick and efficient leaching.

The invention discloses a method for preparing a cucumber seed fermented material, which relates to a method for preparing the fermented material and solves the problems of relatively high toxicity and side effect of the calcium supplementing products prepared by the traditional method. The method comprises the following steps: soaking cucumber seeds, filtering the cucumber seeds, adding a carbon source and an auxiliary material into the soaked cucumber seeds, mixing the carbon source, the auxiliary material and the cucumber seeds to form a mixture, filling the mixture into bags, sterilizing the mixture, cooling the mixture to a room temperature, inoculating a bacterium liquid in each bag, fermenting each bag, drying the mixture in vacuum, crushing the mixture, and thus obtaining the cucumber seed fermented material. The cucumber seed fermented material prepared by the method has the advantages of good calcium supplementing effect, safe administration and no toxic nor side effect.

The invention relates to a PMMA fluorescence copolymer microsphere and a preparation method thereof. The preparation method comprises the steps: firstly, performing vinylation on a micromolecule fluorescence monomer containing amino; then copolymerizing the PMMA fluorescence copolymer microsphere by utilizing a micromolecule unsaturated monomer containing fluorophores and methyl methacrylate. Therefore, the fluorophores can be tightly combined to polymethyl methacrylate resin through chemical bonds; the fluorescence copolymer microsphere has good intermiscibility with solvents of aromatic hydrocarbon, hydrochloric ether, aliphatic ketones, ester and the like and high fluorescence intensity; furthermore, the fluorophores are not prone to drifting away, and fluorescent time is durable. The fluorescent copolymer microsphere disclosed by the invention can be widely applied to the fields of fluorescence probes detection technologies, fluorescent chemical sensors, non-linear optical devices,fluorescent ink, fluorescent additives and the like.

The invention provides a detergent, comprising the following components in parts by weight: 0.4-8 parts of thickener, 0.06-2 parts of cross linker, 2-30 parts of active matter and 60-97.54 parts of water, wherein the thickener is selected from at least one of galactomannan and glucomannan; the cross linker is selected from at least one of boric acid, metaboric acid, metaborate and borate; the active matter is a surfactant of which the hydrophile-lipophile balance value is more than or equal to 12. The detergent is not bonded to packages or hands. Moreover, the invention further provides a preparation method for the detergent.

The invention provides an extraction-back extraction system based on an alkanol eutectic solvent. The extraction-back extraction system comprises a liquid-liquid two-phase extraction system and a liquid-liquid back extraction system, wherein the liquid-liquid two-phase extraction system is composed of an alkanol eutectic solvent and water or a first acidic aqueous solution, and the liquid-liquid back extraction system is composed of the alkanol eutectic solvent and an alkaline aqueous solution or a second acidic aqueous solution. The system is more in variety, low in cost, low in toxicity, high in stability, not easy to dissociate, low in melting point and wide in pH tolerance range. The invention also provides application of the extraction-back extraction system based on the alkanol eutectic solvent in extraction and purification of active compounds. The extraction yield is high, the purification effect is good, and the alkanol eutectic solvent can be repeatedly utilized. The invention also provides an extraction and purification method of the active compound. The method is simple to operate, green, less in extraction and purification steps, less in time consumption, free of special equipment and high in recovery rate of active compounds.

The invention discloses a tablet for relieving physical fatigue. The tablet is prepared from the following raw materials in parts by weight: 400 to 500 parts of taurine, 90 to 110 parts of caffeine, 140 to 160 parts of L-lysine hydrochloride, 45 to 55 parts of inositol, 30 to 40 parts of nicotinamide and 4 to 6 parts of vitamin B6. The invention also discloses a preparation technology of the tablet for relieving the physical fatigue. The preparation technology disclosed by the invention creatively prepares health-care products into tablets and extracts the taurine and the caffeine from naturalsubstances; the safety of the tablet is higher than the safety of artificially synthesized products; the prepared product can significantly relieve the physical fatigue.

The invention discloses functional digestible micelle-state casein powder and a preparation method thereof. The preparation method comprises the following steps: pretreating skimmed milk, preparing micelle-state casein, preparing a compound of functional molecules and the micelle-state casein, and drying the compound, so as to obtain the functional micelle-state casein powder which is relatively good in compressibility, rapid in rehydration and relatively good in solubility and digestibility. The dissolvability and digestibility of the micelle-state casein product can be remarkably improved, so that the micelle-state casein product can be more easily digested into small molecule peptide in an organism. In addition, micellar casein is taken as a carrier, so that loading, delivery and slow release of the functional micromolecules are realized, bioavailability of the functional micromolecules is improved, and health-care effects of the casein and the functional micromolecules are brought into full play. The functional micelle-state casein powder prepared by the preparation method disclosed by the invention has wide application and wide market prospect no matter being used as a health-care product or a raw and auxiliary material in food and medicine industries.

The invention discloses a method for inhibiting a dysosma versipellis explant from contamination and browning. The method comprises the following steps: transplanting a wild dysosma versipellis rhizome which is more than five-year old into clean river sand matrix for preculture; soaking a stem growing strongly into N-long-chain thiocarboxylic acid glycine betaine, washing with distilled water, and brushing the dirt on the surface of the explant; treating the explant by use of anion photocatalysis, sterilizing with sodium hypochlorite, washing with sterile water, sucking moisture on the surface by use of aseptic filter paper until the surface is dry, cutting into squares, and soaking in a heavy metal complexing agent; inoculating onto a modified MS medium for dark culture for two weeks; and then performing bright culture. The method can be used of effectively preventing the dysosma versipellis explant from contamination and browning and providing sufficient aseptic explants for the tissue culture research of the medicinal plant-dysosma versipellis, so that the follow-up tissue culture success rate is ensured, the cost is low and the method is suitable for industrial production.

The invention relates to a TiO2 delustering agent, in particular to a titanium dioxide delustering agent for relieving scaling of a prepolymerization tower. The titanium dioxide delustering agent comprises titanium dioxide particles and a composite layer coating the titanium dioxide particles, and the composite layer sequentially comprises an inorganic silicon compound layer, a metal compound layer and an organic polymer coating layer from inside to outside. The titanium dioxide delustering agent for relieving scaling of the prepolymerization tower has good compactness, can improve the scalingphenomenon and is good in dispersity. The scaling phenomenon in the polymerization tower can be relieved.

The invention relates to a self-flame retardant antibacterial aqueous polyester resin and a preparation method thereof. The resin comprises the following components in parts by weight: 6.0-12.0 partsof organic anhydride, 3.0-5.0 parts of carboxylic acid Schiff base, 3.0-8.0 parts of polybasic acid, 6.0-20.0 parts of polyhydric alcohols, 1.2-3.5 parts of 2-chloro-5-bromobenzoic acid, 1.5-3.5 partsof dimethylolpropionic acid, 2.0-5.0 parts of xylene, 2.0-5.0 parts of a neutralizing agent and 45.0-65.0 parts of deionized water. The self-flame retardant antibacterial aqueous polyester resin provided by the invention is good in coloring property, low in viscosity, high in hardness, good in fullness, good in brightness, and the like, is free of external flame retardant or antibacterial agent when an aqueous coating is prepared, is long in flame retardant and antibacterial effect lasting and high in efficiency, and can be applied to inner and outer wall coatings, aqueous industrial coatings, aqueous wood product coatings, and the like.

The invention discloses a production method of a high-calcium-dissolution cucumber seed drink and belongs to the technical field of agricultural products.The production method comprises: adding acidified water into mature cucumber seed according to a certain proportion for pulping, holding a certain temperature and then filtering, and adding a thickening stabilizer and the like for preparation to obtain the high-calcium-dissolution cucumber seed drink.The problems that traditional powdery products are poor in eating convenience and low in soluble calcium content, thus affecting absorption and utilization, are solved, a novel cool high-calcium drink of all-natural plant calcium origin is developed, and this product has the advantages such as low cost, simple process, instant use and wide applicable range and has a promising market prospect.

The invention discloses a novel ecological, biological and physical purification and disinfection tap water treatment process. Source water sequentially passes through a microbial ecological tank, a multi-medium filter tank, a precision filter, a water molecule cutter and a disinfector; wherein the microbial ecological pool is provided with a porous floating bed, and aquatic plants are planted inholes of the porous floating bed; a plurality of nanofiber carriers and an aeration system are arranged in the microbial ecological tank; the multi-medium filter tank is a multi-stage filter tank, wherein an internal medium of the multi-medium filter tank is one or a mixture of more than two of zeolite mineralization sintered balls, volcanic mineralization sintered balls, medical stone mineralization sintered balls and quartz sand. The novel process for purifying river water, well water and reservoir water into drinking water is not limited by a water source, and any river water, well water and reservoir water can be treated to reach and be superior to the national drinking water standard.

The invention discloses an N-heterocyclic carbene palladium complex crystal, a synthetic method thereof and application thereof in preparation of amide compounds. The structural formula of the complexcrystal is shown in the specification, or the complex crystal is prepared from an N-heterocyclic carbene ligand, Pd(CH3CN)2Cl2 and Ag2O in acetonitrile serving as a solvent. The N-heterocyclic carbene palladium complex crystal is relatively stable, wherein the raw materials used in the synthesis method are cheap and easy to obtain, so that the synthetic method is simple, convenient and easy to implement is simple in post-treamtent and is high in yield; and the N-heterocyclic carbene palladium complex crystal is used for catalyzing carbonylation carbon-nitrogen bond coupling reaction to prepare amide compounds and is high in catalytic activity, easy to operate and high in atom economy.

The invention discloses a preparation method of a nano vaccine with pH and reduction double sensitivity and an obtained product. The nano vaccine is obtained by taking PEI-modified mesoporous silica nanospheres as a raw material, adsorbing a model antigen through electrostatic interaction, and then wrapping an outer layer with a metal phenolic network with reduction sensitive disulfide bonds as aprotection network. The preparation method is simple and convenient to operate, low in energy consumption, environment-friendly and easy to expand production, and the obtained nano vaccine has very good in-vivo long-term stability and biocompatibility and has remarkable treatment and prevention effects on tumors.

The invention discloses an application of indocyanine green-rituximab in preparation of a breast cancer sentinel node tracer. When sentinel node biopsy is carried out via an indocyanine green-rituximab coupled specific tracer, since the specific tracer only fluorescently develops the sentinel node and develops no secondary lymph node, the sentinel node does not need to be found along the fluorescently developed lymphatic in the biopsy, the position of the fluorescently developed sentinel node is detected by an infrared fluorescent development system in a surgery, and the sentinel node positioned by fluorescent development is directly dissected and detected after dissecting and dissociating the peripheral tissues of the sentinel node, so as to avoid the situations of leak detection of the sentinel node and fluorescent development pollution of the peripheral tissues.

The invention relates to an anti-SARS-CoV-2 single-chain antibody, and a preparation method and application thereof. The complementary determining region of the anti-SARS-CoV-2 single-chain antibody comprises a CDR1 and a CDR3, wherein the CDR1 comprises an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2, and the CDR3 comprises an amino acid sequence as shown in SEQ ID NO.3 or SEQ ID NO.4. The anti-SARS-CoV-2 single-chain antibody has high affinity, can be efficiently combined with SARS-CoV-2, is unlikely to dissociate, has a high virus neutralizing activity, can be efficiently expressed in a prokaryotic system, has a standard and controllable expression process, can greatly reduce the production cost, is small in molecular weight, is stable in physicochemical properties and good in heat resistance, can greatly reduce the transportation cost as a prevention and treatment drug, and has an important application value in the field of SARS-CoV-2 prevention and treatment.

The invention belongs to the technical field of adhesives, and particularly relates to a preparation method of a flame-retardant and waterproof adhesive for a corrugated carton. The preparation methodspecifically comprises the following steps: (1) an organic silicon modified emulsion is prepared; (2) a white prepolymer emulsion is prepared; (3) an acrylic modified flame-retardant organic siliconemulsion is prepared; and (4) 110-120 parts of the acrylic modified flame-retardant organic silicon emulsion is put into a high-speed mixer, 1.5-2.3 parts of carnallite powder, 24-27 parts of polyhalite powder and 5-7 parts of a flame retardant are added, even stirring and mixing are conducted, and then the flame-retardant and waterproof adhesive is obtained. The prepared adhesive is transparent,high in adhesion and good in stability, water-based adhesive bonding, quick drying, and acid and alkali resistance are achieved, no organic solvent is contained, the poisonless, tasteless, non-combustible, environment-friendly and pollution-free effects are achieved, and pollution-free production can be achieved. The flame-retardant and waterproof adhesive is suitable for the carton not coated andpolished, and the corrugated carton is protected very well.

The present invention discloses a temozolomide polymer prodrug and a preparation method and an application thereof. The temozolomide polymer prodrug comprises a hydrophilic polymer having a synthesizing terminal of a hydroxyl group and a temozolomide derivative having a synthesizing terminal of a carboxyl group; and the hydroxyl group of the terminal of the hydrophilic polymer reacts with the carboxyl group of the temozolomide derivative, the temozolomide is bonded to the terminal end of the hydrophilic polymer to obtain the temozolomide polymer prodrug. The temozolomide polymer prodrug is prepared by a covalent bond connection of the temozolomide and the polymer and can maintain stability in a body cycle; the temozolomide and the polymer are covalently bonded to form nanomicelles in water, the nanomicelles are not liable to dissociate extracellularly and in blood, and the temozolomide polymer prodrug solves problems that the temozolomide is liable to degrade under physiological conditions of the temozolomide, and drugs are easily leaked in body and short in cycle time, etc.

The invention relates to a phosphorus-nitrogen synergistic self-flame-retardant acrylic emulsion and a preparation method thereof. The method comprises the following steps: synthesizing a phosphorus flame-retardant vinyl monomer, and carrying out an emulsion copolymerization reaction on an acrylate monomer and the vinyl group-containing reactive flame-retardant vinyl monomer containing nitrogen and phosphorus to synthesize the self-flame-retardant acrylic emulsion containing nitrogen and phosphorus. The emulsion has a flame retardation effect without adding other flame retardants, and the flame-retardant phosphorus and nitrogen elements are combined with acrylic resin through chemical bonds and do not dissociate, so the emulsion has long-lasting flame resistance, and can be used in the fireproofing and flame retardation of woodenware coatings, anticorrosive coatings and building interior and exterior coatings.

The invention relates to a rice flour making method for improving the tasty effect of rice flour. The rice flour making method comprises the following steps of (1) making rice into rice flour, addingwater, performing stirring uniformly, adding saccharomyces cerevisiae and alpha-amylase, and performing fermenting at constant temperature to obtain rice milk; (2) adding alkaline protease into the rice milk, and carrying out enzymolysis; (3) adding oleic acid into the zymolytic rice milk for performing pulping, and then adding potato starch and composite phosphate for performing pulping; (4) performing cooking to be pasty; (5) performing extruding to form rice noodles; and (6) performing cooking and cooling on the rice noodles to obtain finished rice flour. A method of combining alpha-amylaseand saccharomycetes is adopted, the alpha-amylase can promote proliferation of the saccharomycetes, the saccharomycetes can produce gas in the fermentation process, and the fresh and wet rice flour can be promoted to form a uniform porous structure; and meanwhile, oleic acid is added in the making process, oleic acid can be combined with starch, and therefore grease and fat-soluble substances canenter the fresh and wet rice flour more easily, so that the tasty effect of the rice flour is enhanced.

The invention discloses a hydrogen methyl esterification reaction catalyst and a method for preparing isononanoic acid. The catalyst comprises an active metal and a ligand, and the active metal is a compound containing cobalt and / or rhodium; the structural formula of the ligand is wherein X 1 、X 2 、X 3 The same or different, all are nitrogen-containing heterocyclic groups. The catalyst is suitable for preparing isononanoic acid methyl ester through hydrogen methyl esterification reaction of diisobutene, methanol and carbon monoxide as raw materials, and then further hydrolyzing to obtain isononanoic acid. The catalyst can improve the conversion rate and the selectivity of the target product, and the yield of the isononanoic acid product reaches 92.9%. The process of the method is relatively simple, the raw material cost is low, the safety is high, and the product yield is high, so the method has good application prospects.

The invention relates to a electroplating solution and an electroplating method and application thereof. The electroplating solution comprises sulfuric acid, copper ions, chloride ions, a tetravalent vanadium compound, a pentavalent vanadium compound, a surfactant and an auxiliary agent. The surfactant comprises any one or a combination of at least two of polyoxyethylene and a derivative thereof, polyoxypropylene and a derivative thereof, a polyoxyethylene-polyoxypropylene copolymer and a derivative thereof or a fatty alcohol ethyoxyl compound. A plating layer formed after electroplating of the electroplating solution has the characteristics of fine plating layer crystals, high ductility and high electrical conduction performance.

The invention discloses a preparation method and application of nanogel with a hunger combined gas therapy anti-tumor function. The nanogel is prepared by the following steps: firstly, performing electrostatic interaction on polyethyleneimine azide grafted with L-arginine and glucose oxidase to form a nano-enzyme compound; and then covering the surface of the nano-enzyme compound with an alkynyl-containing polycarbonate block copolymer, and performing click chemical crosslinking on azide and alkynyl. Compared with the prior art, according to the method disclosed by the invention, specific enzymatic reaction is utilized to consume endogenous glucose and release nitric oxide gas, cancer cells are killed through hunger and gas therapy, besides, the nitric oxide gas can redistribute oxygen in the cancer cells, hypoxia of tumors is relieved, and wide application prospects are realized in the aspects of resisting tumors and inhibiting tumor metastasis.