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Preparation method and application of polypeptide of fermented cucumber seeds

A technology for cucumber seeds and cucumber seed powder is applied in the field of preparation of fermented cucumber seed polypeptides, which can solve the problems of low absorption rate, secondary poisoning, and reproductive system carcinogenic risk, and achieves reduction of activity, promotion of proliferation, prevention and treatment of bone marrow. loose effect

Inactive Publication Date: 2012-08-22
黑龙江省中医研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, inorganic calcium (such as calcium carbonate) is the one with the highest calcium content in all medicinal calcium salts, and is cheap. It is the most widely used calcium supplement at present, and those who lack gastric acid will affect its absorption; organic calcium ( Calcium gluconate) has good water solubility, but low absorption rate; most of the raw materials of traditional Chinese medicine calcium preparations are animal bones, spines of...

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  • Preparation method and application of polypeptide of fermented cucumber seeds
  • Preparation method and application of polypeptide of fermented cucumber seeds
  • Preparation method and application of polypeptide of fermented cucumber seeds

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specific Embodiment approach 1

[0019] Specific embodiment one: the preparation method of the fermented cucumber seed polypeptide in this embodiment is as follows:

[0020] 1. Add the fermented cucumber seed powder into the organic solvent according to the mass-to-volume ratio of 1g: 2-10mL, ultrasonically extract at room temperature for 20-60min or soak at 0-20°C for 18-24h, filter, and discard the filtrate. The filter residue is dried to obtain defatted and dried fermented cucumber seed powder;

[0021] 2. Add the defatted and dried fermented cucumber seed powder into the acetic acid-sodium acetate buffer solution with a pH value of 4.0 to 9.0 or the phosphate buffer with a pH value of 4.0 to 9.0 according to the ratio of mass to volume: 1g: 4 to 20 mL Stir at 4°C for 2-4 hours or soak at 4°C for 24-36 hours, then centrifuge at a speed of 3000-6000 rpm, take the supernatant, and pass the supernatant through an ultrafiltration membrane with a molecular weight of 50,000 Daltons Filtration, and then ultrafil...

specific Embodiment approach 2

[0024] Embodiment 2: This embodiment is different from Embodiment 1 in that the organic solvent described in step 1 is petroleum ether, n-hexane or ether. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0025] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 1, the fermented cucumber seed powder is added to the organic solvent according to the mass-to-volume ratio of 1 g: 8 mL. Others are the same as in the first embodiment.

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Abstract

The invention relates to a preparation method and the application of polypeptide of fermented cucumber seeds. The method includes: firstly, preparing degreased and dried fermented cucumber seed powder; and secondly, adding the degreased and dried cucumber seed powder into buffer solution, stirring or soaking, centrifuging, performing ultrafiltration, concentrating filtrate and drying so that the polypeptide is prepared. The polypeptide of the fermented cucumber seeds is used for preventing and treating osteoporosis. The polypeptide of the fermented cucumber seeds is safe, reliable and non-toxic, is capable of promoting sclerotomal cell proliferation, lowering activity of alkaline phosphatase and improving osteoporosis symptoms, and can play a role in prevention and treatment of osteoporosis and promotion of skeleton growth.

Description

technical field [0001] The invention relates to a preparation method and application of fermented cucumber seed polypeptide. Background technique [0002] Polypeptide is a biologically active substance related to various cell functions in organisms. It is a compound composed of various amino acids in a certain order through peptide bonds, including plant polypeptides and animal polypeptides. According to the research, the natural bioactive peptide has the following characteristics: 1. Naturalness and safety; 2. Has a variety of nutritional components and biological activities; 3. No toxic and side effects. [0003] Microbes have a strong ability to enrich calcium, and can produce some proteins that can complex with calcium, which exist in an organic state through complexation with calcium. More than 90% of the mineral elements in edible fungi exist in an organic state, which has good stability, is convenient for storage, transportation and is better compatible with other in...

Claims

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Application Information

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IPC IPC(8): C07K1/14C12P21/06A61K38/16A61K38/01A61P19/10
Inventor 王伟明陈丽艳曹思思王昶方自若
Owner 黑龙江省中医研究院
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