Immune analysis reagent for magnetic separation alkali phosphatase enzyme mark and analytical detection method for same

An immunoassay and magnetic separation technology, applied in the field of immunoassay reagents and their testing, can solve problems such as pollution and instability of immunological reagents, and achieve the effects of accurate analysis and testing, unstable change of luminescence, and high sensitivity

Inactive Publication Date: 2007-03-14
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of unstable and polluted immunological reagents in the pri...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A magnetically separated alkaline phosphatase-labeled immunoassay reagent, comprising markers, magnetic separation reagents, FITC-antibody markers, standard antigens or antibodies and substrates, wherein the separation reagent is sheep anti-isothiocyanate fluorescence Sulfur-FITC-coated nano-magnetic microbeads, the nano-magnetic microbeads are covered with Fe 3 o 4 or Fe 2 o 3 , containing -OH, -COOH or -NH outside 2 Microbeads of active groups. This example is based on the -NH 2 The group of magnetic beads as an example.

[0029] This example takes the gonad eight items (FSH, LH, E2, P, T, PRL, HCG, β-HCG) as an example

[0030] 1. Preparation of goat anti-FITC polyclonal antibody coated on the surface of nano-magnetic microbeads:

[0031] The preparation method of nano magnetic beads is prepared according to the Chinese patent, the patent number is CN92105584.6, and the inside of the magnetic beads is coated with Fe 2 o 3 The surface contains -NH 2 The grou...

Embodiment 2

[0107] Take the eight items of the thyroid gland as an example:

[0108] The preparation method of the goat anti-FITC polyclonal antibody coated on the surface of nano-magnetic microbeads is the same as in Example 1, the surface of the magnetic beads is a group containing -COOH, and its content is 0.3eqm / g, and the chromogenic substrate is phenolphthalein monophosphate , during the incubation process, after adding the alkaline stop solution, alkaline phosphatase catalyzes the chromogenic substrate monophosphate phenolphthalein to turn pink, and measures the color intensity of the reaction product with a measuring instrument, and then draws according to the concentration of the standard antigen or antibody The result of the test substance.

[0109] T3

T4

FT3

FT4

TSH

TGA

TMA

TRAb

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send

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put

waived

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PUM

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Abstract

The magnetic separated AKP-labeled immunity analysis reagent comprises: the AKP label, the magnetic separation reagent as nano magnetic micro-ball covered by FITC, the FITC-antibody label, and the standard antigen or antibody and substrate. Wherein, the substrate can be PMP, AMPPD or APS-5. This invention is reliable and nun-polluted, and can detect fast and precisely.

Description

technical field [0001] The invention relates to an immunoassay reagent and a testing method thereof, in particular to an immunoassay reagent labeled with magnetic separation alkaline phosphatase and an analyzing and testing method thereof. Background technique [0002] At present, the clinical methods for measuring these trace active substances in China mainly include radioimmunoassay method, enzyme immunoassay method, enzymatic chemiluminescence or acridinium ester-labeled direct chemiluminescence method, triple pyridine ruthenium-labeled electrochemiluminescence method, etc. ; Radioimmunoassay is the use of radioisotope I 125 As a tracer, labeled on the antigen or the corresponding antibody, on the gamma ray detector, through the I 125 Although the determination of the radiation intensity to determine the content of the substance to be tested is a good solution to the quantitative analysis of trace active substances, its defects are obvious: radioisotope I 125 As a trace...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/52G01N21/64
Inventor 饶微宋洪涛李婷华邵佳阎颖王丽蓉黄国光刘海燕
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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