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Application of cellulose-based carbon quantum dot preparation in organophosphorus pesticide detection

An organophosphorus pesticide and carbon quantum-based technology, applied in the field of fluorescent probes, can solve problems such as unresearched carbon quantum dots, and achieve the effects of universality, low cost and simple operation method.

Active Publication Date: 2021-10-26
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Carbon quantum dots that can directly detect organophosphorus pesticides in water environment have not yet been developed

Method used

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  • Application of cellulose-based carbon quantum dot preparation in organophosphorus pesticide detection
  • Application of cellulose-based carbon quantum dot preparation in organophosphorus pesticide detection
  • Application of cellulose-based carbon quantum dot preparation in organophosphorus pesticide detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of fluorescent carbon quantum dots

[0047] The preparation method is as follows:

[0048] 0.5 g of microcrystalline cellulose and 0.5 g of aspartic acid were placed in a mortar, repeatedly ground and mixed well. Add 35 ml of ultra-pure water to the beaker, stirred and ultrasonically 30min, and the sufficiently dispersed solution was transferred to the polytetrafluoroethylene liner of the high-pressure water heating reaction kettle, and the oven was preheated to 190 ° C, and the reaction was 12 h. After the reaction, the reaction cooled to room temperature, and the solution was taken out again to treat 30 min and taking the supernatant, the resulting water dispersion solution was centrifuged at 7000 rpm for 30 min After centrifugation by 0.22 μm filtration. A carbon quantum point of blue fluorescence can be obtained by dialysis in a dialysis bag having a molecular interception of 1000. Finally, the dialysis solution was placed in a cryogenic dryer for ...

Embodiment 2

[0049] Example 2 Two-phase structural fluorescent microgel TEM

[0050] The carbon quantum dot solution produced in Example 1 was added dropwise on the copper net, which was placed in a constant temperature incubator for 12 h. TEM map to shoot figure 2 As shown, the carbon quantum dot distribution is uniform, and the average size is 10 nm. image 3 Infrared spectrum for the prepared carbon dot, such as image 3 Down, 3200cm -1 The wide suction peak corresponds to the bending vibration of N-H. C = O stretch vibration, corresponding 1671cm -1 Strong peak; due to C-N telescopic vibration, will be at 1375cm -1 A peak appears. Indicates that carbon quantum dots indicate link -NH 2 And the functional group such as -COOH.

[0051] Ultraviolet absorption spectrum for NPP ( Figure 4 ) Conduct analysis, and its absorption peak is at 312 nm.

[0052] The carbon dots synthesized in Example 1 were fluorescence measured to obtain a carbon dot fluorescence excitation, emission map, and compared to...

Embodiment 3

[0053] Example 3 Sensitivity detection of fluorescent carbon quantum dots on killing phosphorus

[0054] During this test, four control groups were set. The first group was dissolved in 2 ml ultrapure water at a concentration of 1 mg / ml in Example 1 in Example 1; the second group, taking 10 in advance 10 -3 Mol / L of NPP solution 200 μL and 2 mg of carbon dot powder is dissolved in 1.8 mL of ultrapure water; group three, alkaline phosphatase is diluted with PBS buffer (0.1 mol / L) on the ultra-clean station, add In the same solution as the group, 37 ° C water bath was cultured for 30 min, of which the concentration of Alp was 3 U / mL; the operation step of the fourth group was similar to the third group, and the difference is that 1 μL 1 mg / ml of the solution is added to the solution before adding ALP. The sulfur sulfur phosphorus standard solution was added 37 ° C for 30 min after adding Alp. Finally, the four groups of solutions were analyzed at 330 nm excitation waveleng...

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Abstract

The invention relates to the technical field of carbon quantum dots, in particular to preparation of cellulose-based carbon quantum dots and application of the cellulose-based carbon quantum dots in the field of organophosphorus pesticide (OPs) detection. The nitrogen-doped blue fluorescent carbon dots are synthesized by taking microcrystalline cellulose and aspartic acid as raw materials through adoption of a one-step hydrothermal method. Characterization is carried out by means of a transmission electron microscope, an ultraviolet spectrum, a fluorescence spectrum and the like. On the basis of an internal filtering effect (PER), by virtue of an enzymatic reaction of decomposing 4-nitrophenyl sodium phosphate (NPP) through alkaline phosphatase (ALP), carbon dot fluorescence is quenched by taking NPP as an absorber, and the fluorescence is recovered along with continuous generation of a product 4-nitrophenol (NTP). The invention also provides a novel organophosphorus pesticide detection method, when OPs exists in a detection solution, the decomposition progress of NPP can be influenced, and the fluorescence recovery level has different degrees of differences. Through the change of the fluorescence intensity, whether the organophosphorus pesticide exists in the daily water or agricultural products or not is visually judged, so that the purposes of protecting the environment and maintaining the body health are achieved.

Description

Technical field [0001] The present invention relates to the field of fluorescence probe, particularly a method for preparing an organophosphorus pesticides for detecting fluorescence quantum dots carbon. Background technique [0002] The information disclosed in the background is disclosed only to increase the understanding of the overall background of the present invention, and is not necessarily considered to admit or imply that the information is intended to be a prior art known to those skilled in the art. [0003] Carbon quantum dots (CQDs) as a carbon-based fluorescent nanoparticle material family Ranran star, research reports in recent years the trend showed exponential growth, primarily due to the many advantages provided in point C, such as low cost, easy preparation process, good biocompatibility, luminescent species diversity. Currently, the carbon points in many areas has demonstrated good prospect, for example: Energy photovoltaic device, encryption and security info...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402Y02A50/30
Inventor 张献纪骁元张联辉卢倩姚金水
Owner QILU UNIV OF TECH
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