Magnetic separation enzyme-linked immune detecting method for thyroid gland peroxidase antibody

Abstract

This invention provides a human hypothyroid peroxid enzyme antibody magnetic separation enzyme immune measurement method, whose steps are the following: to add sample to react with immune; to wash and to join two anti-enzyme indicate reagent and to wash again; to add monophosphatase fenolftaleina substrate solvent; to terminate color forming and to read absorbing value. The advantages in this invention are the following: it uses reformed human hypothyroid peroxid enzyme antigen fold immune magnetism micro ball as fixed phase and uses alkalescence phosphate enzyme as mark enzyme. Through the quantitative measurement of peroxid enzyme antigen of the hypothyroid patient and normal serum and other clinic symptom, it can judge whether the patient has got the self-immune hypothyroid illness.

Description

[0001] The invention relates to the field of immunoassay and analysis, in particular to an enzyme-linked immunoassay method for magnetic separation of human thyroid peroxidase antibody. Background technique

[0002] As we all know, the serum of patients with thyroid autoimmune diseases usually contains thyroid microsomal antigen. Since the discovery of this antigen more than 20 years ago, its basic biological properties have not been clearly studied. With the development of molecular biology techniques, many studies have confirmed that the main component of thyroid microsomal antigen is thyroid peroxidase (NAOKATA YOKOYAMA et al., Journal of clinical Endocrinology and Metabolism). Thyroid peroxidase is a membrane-integrated protein located on the apical membrane of follicular cells. The enzyme uses heme as a prosthetic group with a molecular weight of about 101KD. The traditional detection of thyroid autoimmune diseases is usually based on thyroid microsomal antibodies, radioi...