Microbial fodder additive

A microbial feed and additive technology, applied in animal feed, animal feed, bacteria, etc., can solve problems such as difficult quality control and product instability, and achieve the effects of improving feed conversion rate, product quality, and taste

Inactive Publication Date: 2007-03-28
利耀铭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1579200A discloses a multi-effect microbial feed additive obtained by fermentation with a single lactic acid bacillus, and the product is a liquid fermentation broth, and the product can continue to ferment at a certain temperature, so the product is unstable and difficult to control quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Source, cultivation and preservation of Saccharomyces cerevisiae Li1012 (Saccharomyces cerevisiae Li1012)

[0028] Source: wild yeast isolated and selected from the soil of litchi orchards planted in Baiyun District, Guangdong. It was identified as Saccharomyces cerevisiae Li1012 cultured by China Center for Typical Culture Collection, Wuhan University, China:

[0029] The medium used: peel the potatoes, cut them into small pieces, add water to boil for 30-45 minutes, filter, mix the filtered potatoes with glucose and water according to the weight ratio of 10:1:50, and sterilize under an atmospheric pressure for 18-45 minutes. 30 minutes.

[0030] Strain cultivation: Saccharomyces cerevisiae Li1012 was inoculated into the above-mentioned medium, and cultured in an incubator at 32-35° C. for 3-5 days to obtain yeast strains.

[0031] Strain preservation:

[0032] Slant medium formula: yeast extract 0.5-0.8%, peptone 1.0-1.3%, glucose 0.5-0.8%, white sugar 1....

Embodiment 2

[0034] The preparation of embodiment 2 feed additives

[0035] Step 1: (1) Enlarged culture of pure species: Weigh 1000g of bran and 700g of water, stir and mix to make solid medium A, pack in 500ml Erlenmeyer flasks, each bottle contains 300g of medium A, and sterilize at 0.1MPa Take Trichoderma, Aspergillus, Actinomycetes, and Bacillus strains for 30 minutes, and inoculate them in the Erlenmeyer flasks with medium under sterile conditions, place them in an incubator, and cultivate them at 30-33°C for 72 Hour;

[0036] (2) Liquid fermentation culture:

[0037] Weigh 5g of yeast extract, 10g of peptone, 8g of glucose, 12g of white granulated sugar, and 965g of water, stir and dissolve, then the pH value is 6.5 as a liquid medium, and divide them into 500ml triangular flasks, 250ml per bottle, and sterilize at 0.1MPa for 30 minutes , take the Saccharomyces cerevisiae strain of Example 1 and inoculate it in a Erlenmeyer flask equipped with a liquid medium under aseptic conditi...

Embodiment 3

[0053] Embodiment 3 feeds piglet stage test situation with feed additive

[0054] Test method: 30 piglets were randomly divided into three groups, 10 pigs in each group, including 7 male pigs and 3 sows. Feeding time, method and conditions are the same

[0055] Test group 1: 2% of the fermented feed additive in Example 2 of the present invention, and other mixtures of any proportion such as corn flour, bran, rice bran, soybean meal, etc. were fed as feed

[0056] Test group 2: 4% of the fermented feed additive in Example 2 of the present invention, and other mixtures of any proportion such as corn flour, bran, rice bran, soybean meal, etc. were fed as feed

[0057] Control group 3: Feed with any mixture of corn flour, bran, rice bran, soybean meal, etc.

[0058] test results:

[0059] Feeding days Total weight of piglets before test Total weight after test Weight gain Average daily gain per head Diarrhea

[0060] days Kg Kg Kg g head

[0061] Test group ...

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PUM

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Abstract

The present invention relates to feed additive containing microorganism. It is made up by using trichodermin, aspergillus, actinomycetes, bacillus and saccharomyces cerevisiae Li1012 through the processes of pure strain amplification culture, liquid fermentation culture and solid two-stage fermentation culture.

Description

technical field [0001] The present invention relates to a feed additive containing active microorganisms. Background technique [0002] Microbial feed additives are widely used at home and abroad because of their advantages of safety, environmental protection, no side effects, disease resistance and growth promotion. However, feed additives produced by different manufacturers using different strains and different production processes have different effects. A multi-effect microbial feed additive disclosed in CN1579200A is fermented with a single lactic acid bacillus, and the product is a liquid fermentation broth, which can continue to ferment at a certain temperature, so the product is unstable and difficult to control quality. For this reason, it is necessary for us to develop new feed additives that are suitable for the vast rural areas of our country without adding any chemical substances and hormones. Contents of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20A23K10/18
Inventor 利耀铭
Owner 利耀铭
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