Microorganism flocculant producing strain by using bean dregs and process for producing same

Abstract

This invention relates to a microbial flocculating agent. The invention isolates a high-yield bioflocculation microorganism-Paenibacilluspolymyxa GA1 CCTCC M 206017, and utilizes bean dregs as the substituted culture medium to synthesize the flocculating agent. The culture process comprises: processing the bean dregs at pH11.0 by boiling at high temperature and pressure, adjusting the pH to pH6.0-8.0, inoculating with 0.5-2% seed solution, applying two-stage fermentation process, wherein: at the early state of 24 hours, the culture temperature is 30deg C and the shaker speed is 150r/min; and at the late stage of 32 hours, the culture temperature is 25deg C and the shaker speed is 100r/min. By applying the two-stage fermentation process, the bacteria can make use of nutrients to synthesize the flocculating agent at a maximu rate, with the yield increaed to 7.86g/L and culture cycle decreased to 56h. By using the bean dregs and the two-stage fermentation process, it can lower the cost for producing the microbial flocculating agent, improve the yield, and realize the massive industrial production of microbial flocculating agent.

Description

technical field [0001] The invention relates to a water treatment agent-flocculant used in industries such as industrial waste water treatment, food production and fermentation, in particular to a microbial flocculant. Background technique [0002] Flocculants are widely used in industries such as industrial wastewater treatment, food production and fermentation. Generally, flocculants are divided into three categories: (1) inorganic flocculants, such as aluminum sulfate, polyaluminum chloride, polyferric sulfate, etc.; (2) organic synthetic polymer flocculants, such as polyacrylamide and its derivatives, poly Ethylene imine, polystyrene sulfonate, etc.; (3) Natural polymer flocculants, such as modified starch, polyglucosamine, chitosan, sodium alginate, chitin and microbial flocculants. Among these flocculants, organic synthetic polymer flocculants are widely used in actual production because of their good flocculation effect and low cost. Polyacrylamide is a commonly use...

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Problems solved by technology

[0004] However, at present, most of the research on microbial flocculants at home and abroad is in the stage of laboratory research, and has not yet reached the stage of practical application and industrial production.

The key to restricting its development is that the output is too low or the production cost is too high

For example, the flocculant-producing bacteria screened by Ye Jingjing et al. had an output of 1.985g / L; Zhou Xu et al. used Pseudomonas GX-4 to produce flocculant from fishmeal processing wastewater, and the yield was only 0.612g / L; Wang Zhen et al. The production of flocculant-producing bacteria is 0.5-0.9g / L; a strain of Bacillus producing flocculant screened by Ma Xiaona of China Agricultural University, although its production is as high as 12.48g / L, is produced by pure chemical reagents citric acid and gluten. Amino acid, etc. are used as the medium, so the cost is high

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