Skin-care product contg. tortoise skin collagen composition, and its prepn. method
A composition and technology of skin care products, applied in the field of skin care products, can solve the problems of short moisturizing time, anti-wrinkle and insignificant anti-aging effects, etc.
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Embodiment 3
[0045] Embodiment 3: the preparation of Brazilian tortoise skin collagen
[0046] 1: Rinse 1 kg of frozen Brazilian tortoise skin with tap water at 15°C, remove scales and subcutaneous tissue, and then rinse with deionized water.
[0047] 2: Use 5% Na 2 CO 3 Soak and rinse the tortoise skin with 10 liters of solution for about 5 days, and then fully wash the alkali-treated tortoise skin with water until it is neutral.
[0048] 3: Add 5 liters of acetic acid with pH 2.5 and 10 grams of pepsin to the tortoise skin treated above, enzymolyze at 4°C for 2 days, filter with gauze, enzymolyze the residue again, treat as above, and combine the filtrates.
[0049] 4: Centrifuge with a 5000r / min centrifuge, remove the residue and take the clear liquid, put the centrifuged clear liquid into a dialysis bag, dialyze with deionized water, change the water every 3 to 4 hours, until the 10% AgNO 3 There was no precipitation in the solution test, and the pH value was around 6.5-7.0 at this ...
Embodiment 4
[0051] Embodiment 4: the preparation of tortoise skin collagen
[0052] 1: Rinse 500 grams of fresh tortoise skin with tap water at 20°C, remove the subcutaneous tissue, and rinse with deionized water.
[0053] 2: Soak and rinse the tortoise skin with 1 liter of 0.5% NaOH solution for about 1 day, and then fully wash the alkali-treated tortoise skin with water until it is neutral.
[0054] 3: Add 2.5 liters of neutral water and 50 grams of papain to the tortoise skin treated above, enzymolyze at 4°C for 2 days, filter with gauze, enzymolyze the residue again, treat as above, and combine the filtrates.
[0055] 4: Centrifuge with a centrifuge at 10000r / min, remove the residue and take the clear liquid, put the centrifuged clear liquid into a dialysis bag, dialyze with deionized water, change the water every 3 to 4 hours, until the 10% AgNO 3 There was no precipitation in the solution test, and the pH value was around 6.5-7.0 at this time.
[0056] 5: Enzymatic hydrolysis of t...
Embodiment 5
[0057] Embodiment 5: the preparation of tortoise gum cosmetic
[0058] Oil phase: Add the oil phase raw material in Example 1 or 2 into a melting pot, heat it, and heat it to 70-75°C under constant stirring to make it fully melt. Avoid overheating and prolonged heating to prevent oxidative deterioration of raw materials. Easily oxidized oils, preservatives and emulsifiers can be added to the oil phase before emulsification, and the emulsification can be carried out after dissolving evenly.
[0059] Water phase: first add 30% to 50% deionized water into the melting pot, and at the same time add the water phase raw materials in Example 1 or 2, heat to 90-100°C under stirring, keep for 20 minutes to sterilize, and set aside. If the formula contains water-soluble polymer, it should be prepared separately, dissolved in water, stirred at room temperature to make it evenly swell, to prevent agglomeration, if necessary, it can be homogenized, and added to the water phase before emuls...
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