1,3-propylene glycol and 2,3-butanediol production method using glecerol by microorganism two-step fermentation method

A technology of microorganisms and butanediol, which is applied in the direction of fermentation, etc., can solve the problem of low yield, achieve the effects of reducing production costs, increasing product concentration, and improving raw material utilization

Active Publication Date: 2005-01-26
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its low yield in 1,3-propanediol fermentati

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] (1) Bacteria: Klebsiella pneumoniae M5al

[0016] (2) culture medium

[0017] Medium components Seed medium Fermentation medium Trace element solution

[0018] ( / l) ( / l) (mg / l)

[0019] Glycerin 20g 20-80g ZnCl 2 70

[0020] K 2 HPO 4 ·3H 2 O 4.45g 2.225g MnCl 2 4H 2 O 100

[0021] (NH 4 ) 2 SO 4 2.0g 2.0g H 3 BO 3 60

[0022] K H 2 PO 4 1.3g 0.65g CoCl 2 ·6H 2 O 200

[0023] MgSO 4 ·7H 2 O 0.2g 0.2g NiCl 2 ·6H 2 O 25

[0024] Yeast powder 1.0g 1.5g NiCl 2 ·H 2 O 27.64

[0025] Trace element solution 2ml 2ml Na 2 MoO 4 2H 2 O 35

[0026] CaCO 32.0g CuCl 2 ·H 2 O 20

[0027] Defoamer 0.1ml CuSO 4 ·5H 2 O 29.28

[0028] Concentrated hydrochloric acid (37%) 0.9ml

[0029] (3) Training method:

[0030] A. Seed culture:

[0031] The strains preserved in glycerol tubes were transferred to LB slant and activated at 30°C for 12 hours. Seed...

example 2

[0038] (1) bacterial classification: with example 1

[0039] (2) culture medium: with example 1

[0040] (3) Training method:

[0041] A. Seed culture:

[0042] The strains preserved in glycerol tubes were transferred to LB slant and activated at 30°C for 12 hours. Seed culture adopts aerobic culture, using a 500ml Erlenmeyer flask with a liquid volume of 100ml. The culture temperature was 30° C., and the shaking table rotated at 140 rpm.

[0043] B. Fermentation culture: a 5L fermenter was used for fermentation culture, with a liquid volume of 4L, a culture temperature of 37°C, and a pH value of 7. During the fermentation process, the initial fermentation medium containing 30g / l industrial glycerin was used, and a mixed solution of 800g / l glycerin and 80g / l glucose was added after 4 hours of fermentation. Where glycerol: glucose = 10:1 (w / w)

[0044] (4) Fermentation method:

[0045] Klebsiella fermentation produces 1,3-propanediol and 2,3-butanediol: During the fermen...

example 3

[0049] (1) bacterial classification: with example 1

[0050] (2) culture medium: with example 1

[0051] (3) Training method:

[0052] A. Seed culture:

[0053] The strains preserved in glycerol tubes were transferred to LB slant and activated at 30°C for 12 hours. Seed culture adopts aerobic culture, using a 500ml Erlenmeyer flask with a liquid volume of 100ml. The culture temperature was 30° C., and the shaking table rotated at 140 rpm.

[0054] B. Fermentation culture: a 5L fermenter was used for fermentation culture, with a liquid volume of 4L, a culture temperature of 40°C, and a pH value of 7. During the fermentation process, the glycerol fermentation liquid containing 80g / l glycerol was used as the initial fermentation medium, and 1000g / l glycerol and 100g / l glucose mixed solution were added after 5 hours of fermentation, wherein glycerol: glucose=10:1 (w / w ).

[0055] (4) Fermentation method:

[0056] Klebsiella fermentation produces 1,3-propanediol and 2,3-buta...

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Abstract

The invention discloses a method for simultaneously preparing 1,3-propylene glycol and 2,3-butylene glycol from glycerol by using microbe employing prior-stage anaerobic and later-stage aerobic two-stage fermentation process, which belongs to the field of the bio-chemical engineering technology. In the fermentation process, the seed liquid is added in the primary fermentation medium containing industrial glycerol or glycerol fermentation liquid, after 3-5 hours, the mixed liquid of glycerol and glucose is added in the form of the flow. The product concentrations of 1,3-propylene glycol and 2,3-butylene glycol are controlled by adjusting the air flow in the two-stage fermentation process and controlling the fermentation temperature. When the fermentation process is over, 1,3-propylene glycol and 2,3-butylene glycol are separated and purified by desalting, distilling, and vacuum rectification. The preparing method can improve the fermentation concentration and the yield of 1,3-propylene glycol and the production intensity, at the same time, 2,3-butylene glycol with higher concentration can be obtained with high raw material availability and low cost.

Description

technical field [0001] The invention belongs to the field of biochemical technology, in particular to a microbial two-stage fermentation method for producing 1,3-propanediol and 2,3-butanediol from glycerin. Background technique [0002] 1,3-Propanediol (PDO) is an important chemical raw material, which can be used as an organic solvent in ink, printing and dyeing, coating, lubricant, antifreeze and other industries. The most important use of 1,3-propanediol is as a monomer for polyester and polyurethane synthesis, especially polytrimethylene terephthalate (PTT), which is polymerized with terephthalic acid, shows a ratio of 1,2- Polymers synthesized from propylene glycol, butanediol, and ethylene glycol have better performance. At present, the world consumes tens of millions of tons of polyethylene terephthalate (PET) every year, and the chemical stability and biodegradability of PTT are comparable to PET, but the pollution resistance, toughness, resilience and UV resistanc...

Claims

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Application Information

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IPC IPC(8): C12P7/18
Inventor 刘德华孙燕程可可刘卫斌刘宏娟
Owner TSINGHUA UNIV
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