308 results about "Yarrowia sp." patented technology

The invention belongs to the technical field of bioengineering, and discloses a recombinant yarrowia lipolytica capable of efficiently and rapidly producing sclareol, which is characterized in that optimized sclareol synthase related coding genes ScLPPS, ScSCS, GGPPS1, GGPPS2, ERG20 and tHMGR coding genes are inserted into a yarrowia lipolytica genome; inserting optimized pentadienol utilization pathway coding genes such as IPK, THIM and IDI coding genes; and inserting an optimized ATP: citrate lyase coding gene ACLY to obtain the ATP: citrate lyase coding gene ACLY. The recombinant yarrowia lipolytica is based on knockout of a yarrowia lipolytica strain responsible for coding a non-homologous recombination gene ku70, so that the homologous recombination capability of the yarrowia lipolytica strain is enhanced, gene integration is realized through the homologous recombination function of the yarrowia lipolytica, the genetic stability of the introduced gene can be greatly improved, and the recombinant yarrowia lipolytica strain has a good application prospect. The construction method of the recombinant yarrowia lipolytica strain is efficient and simple to operate.

The invention provides a genetically engineered bacterium, a preparation method thereof and application of the genetically engineered bacterium in synthesis of sclareol. The genetically engineered bacterium comprises a CcLPPS gene derived from Cistanus crenatus, and a CcLPPS gene derived from Cistanus crenatus, the genetically engineered bacterium is selected from yarrowia lipolytica. According to the invention, the stably expressed sclareol efficient de novo synthesis strain is constructed, and no organic reagent or any path intermediate substance is added in the whole fermentation process, so that the production cost is greatly reduced, and the subsequent separation and purification steps are simplified. The engineering yarrowia lipolytica strain provided by the invention is high in production intensity and simple in fermentation process, has a relatively good application prospect, and lays a foundation for producing sclareol by a microbial fermentation method.

The invention discloses a mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as a construction method and application thereof, the recombinant strain exogenously expresses mesophilic original fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from phaffia rhodozyma, and efficient synthesis under the condition of 30 DEG C is realized; secondly, a metabolic pathway of the recombinant strain is optimized based on a metabolic flow push-pull strategy, and isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), plant alkene dehydrogenase (CarB) and bifunctional plant alkene synthase (CarRP) are overexpressed, so that the flux of a precursor is increased; finally, gene multi-copy of astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from phaffia rhodozyma is carried out, and the carotenoid production capacity of yarrowia lipolytica is further improved. The construction method of the mesophilic yarrowia lipolytica is simple, efficient synthesis of carotenoid can be better achieved at the higher temperature, the fermentation cost is greatly reduced, and the industrial production potential is achieved.

The invention belongs to the technical field of genetic engineering, and discloses a Yarrowia lipolytica engineering strain capable of efficiently producing eicosapentaenoic acid through a cofactor strategy, Yarrowia lipolytica Po1f is used as an original strain, and genes of delta 9 elongase, delta 8 desaturase, delta 5 desaturase and delta 17 desaturase are over-expressed, so that the eicosapentaenoic acid can be efficiently produced. And simultaneously overexpressing glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME) to obtain the gene. The construction method is suitable for constructing a genetic engineering strain for producing eicosapentaenoic acid, the aim of simply, conveniently, effectively and accurately constructing the strain is achieved, the constructed engineering strain is further applied to production of eicosapentaenoic acid, and an effective way is provided for commercially producing eicosapentaenoic acid by applying yarrowia lipolytica.

The invention provides recombinant yeast for producing bakuchiol as well as a construction method and application of the recombinant yeast. Wherein the recombinant yeast is Yarrowia lipolytica containing isopentenyl transferase, and the recombinant yeast is a Yarrowia lipolytica containing isopentenyl transferase. Wherein a gene for coding the isopentenyl transferase is derived from Psoralea coryllifelia Linn (Psoralea coryllifelia Linn), and the isopentenyl transferase is used for coding the isopentenyl transferase. By utilizing the recombinant yeast provided by the invention, the bakuchiol with high yield can be efficiently and quickly prepared through a biosynthesis method, and a technical support is provided for subsequent industrialization of the bakuchiol.

The invention provides recombinant yarrowia lipolytica as well as a construction method and application thereof. According to the method disclosed by the invention, beta-carotene synthesis related genes are transferred into a yarrowia lipolytica starting strain to obtain the recombinant yarrowia lipolytica with the capacity of high yield of beta-carotene; furthermore, related genes are introduced on the basis of the recombinant yarrowia lipolytica with high yield of beta-carotene, so that efficient conversion from beta-carotene to zeaxanthin is successfully realized.

The invention relates to the field of microbial fermentation, in particular to an ethyl carbamate-reducing Caribik Yarrowia spp. And application of the Caribik Yarrowia spp. The invention discloses Meyerozyma caribba P3, the enzyme activity of ethyl carbamate hydrolase of the Meyerozyma caribba P3 is high and can reach 980.40 U, and when the Meyerozyma caribba P3 is applied to yellow wine fermentation, 49.5% of yellow wine EC can be degraded. The yeast strain for degrading the ethyl carbamate in the fermented food has the advantages of being high in fermentation capacity, capable of tolerating low-acid, high-sugar and 12% alcohol environments and the like, and a valuable strain source is provided for degrading the ethyl carbamate in the fermented food through a microbiological method.

The invention relates to the technical field of microbial fermentation, in particular to a Jianliermondii YT-60 strain and application of the Jianliermondii YT-60 strain in lemon wine, the Jianliermondii YT-60 strain is preserved in Guangdong Microbial Culture Collection Center on April 3, 2025, the preservation number is GDMCC No: 66091, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, and the preservation number is CGMCC No: 66091. The preservation address of Guangdong Institute of Microbiology is 510070. The invention provides a novel fruit wine fermentation strain, the strain has better tolerance to pH, sugar degree, ethanol and SO2, and the total ester yield is as high as 2.60 g / L. The lemon wine fermented by combining the strain provided by the invention with saccharomyces cerevisiae has more unique flavor than the lemon wine fermented by single saccharomyces cerevisiae.

The invention relates to the technical field of functional lipid preparation, in particular to high-purity nannochloropsis oculata polar lipid enriched by an enzymic method and a preparation method of the high-purity nannochloropsis oculata polar lipid. The method comprises the following steps: specifically and selectively hydrolyzing triglyceride by using immobilized Lip2 lipase to generate glycerol and free fatty acid, retaining and enriching polar lipid components, and carrying out centrifugal filtration and molecular distillation to obtain the high-purity nannochloropsis oculata polar lipid. The invention provides a method for preparing high-purity polar lipid by performing enzymolysis on nannochloropsis oculata oil by using lipase Lip2 produced by yarrowia lipolytica, which maximally retains the polar lipid and obtains a high-purity polar lipid product through the specific catalytic action of Lip2 lipase. The method which is mild in reaction condition, environment-friendly, simple in step and high in purity is provided for efficient enrichment of the nannochloropsis oculata polar lipid, the prepared polar lipid product is extremely high in purity, and the blank of efficient preparation of the nannochloropsis oculata polar lipid in the prior art is filled.

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention belongs to the technical field of biology, and discloses an exogenous cofactor enhanced yarrowia lipolytica strain, a method and application in efficient synthesis of EPA (eicosapentaenoic acid) and lipids.The chassis strain of the yarrowia lipolytica strain is yarrowia lipolytica Po1f, desaturase is partially prolonged in an overexpression fatty acid synthesis route in the strain, and the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids. The desaturase gene mainly comprises a delta 9 elongase gene, a delta 8 desaturase gene, a delta 5 desaturase gene and a delta 17 desaturase gene, and G6PDH and malic enzyme are overexpressed at the same time on the basis. The strain provided by the invention improves the EPA and lipid yield of yarrowia lipolytica; the method is suitable for constructing a genetic engineering strain with high yield of EPA and lipid, provides a basis for producing grease rich in EPA from yarrowia lipolytica, and solves the problems of non-sustainability, high cost and the like caused by obtaining EPA from fish.

The invention belongs to the field of polypeptides, and particularly relates to an antibacterial peptide derived from marine microorganisms, an antibacterial composition and application. Based on a deep learning algorithm, five potential antibacterial peptides are mined from a global marine microbiome database, and the five antibacterial peptides are expressed in yarrowia lipolytica. Experimental tests show that the five antibacterial peptides have differentiated antibacterial effects on different bacteria, and the antibacterial peptide shown in SEQ ID NO: 3 can have an obvious inhibition effect on escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, has low hemolysis and has a positive application prospect. The antibacterial peptide disclosed by the invention has the potential of further developing novel antibacterial drugs or antibacterial additives.

The invention relates to the field of bioengineering, in particular to a construction method and application of recombinant yarrowia lipolytica with high succinic acid yield. The recombinant yarrowia lipolytica disclosed by the invention simultaneously expresses a reduced tricarboxylic acid pathway related gene, a glyoxylic acid circulation pathway related group, a heterologous dicarboxylic acid transport related gene and a mitochondrial transport related gene, and an endogenous dicarboxylic acid transport protein coding gene is also knocked out; and a natural promoter of an isocitrate dehydrogenase coding gene IDH1 is replaced. The recombinant yarrowia lipolytica constructed by the invention can produce 112.85 g / L of succinic acid after fed-batch fermentation under the condition of not controlling pH, and efficient synthesis of succinic acid in the yarrowia lipolytica is realized.

The invention discloses a high-yield delta-tocotrienol genetically engineered bacterium and application thereof, and belongs to the technical field of gene recombination. The invention discloses a genetically engineered bacterium for high yield of delta-tocotrienols, which is characterized in that yarrowia lipolytica ATCC MYA-2613 is used as an original strain, and a ku70 gene is knocked out; the gene is used for expressing and coding 4-hydroxyphenylpyruvate dioxygenase, the gene is used for coding uromelanic acid phytate transferase and the gene is used for coding tocopherol cyclase; intensifying a shikimic acid pathway; a mevalonic acid pathway is strengthened; an isopentenol path is introduced; carrying out protein fusion on the HPD and the HPT; truncating a signal peptide of a gene encoding a tocopherol cyclase and back-filling a leucine defect. The genetically engineered bacterium constructed by the invention has industrial stability, can be amplified by more than 10 times in a 2L fermentation tank level compared with a shake flask level, can produce 616.45 mg / L of delta-tocotrienol after being cultured for 240 hours, and has industrial application prospects.

The invention discloses a yarrowia lipolytica engineering strain with high yield of resveratrol as well as construction and application of the yarrowia lipolytica engineering strain, and belongs to the technical field of synthetic biology and metabolic engineering. The invention provides a yarrowia lipolytica engineering strain. A 4-coumaric acid-CoA ligase gene, a multi-copy resveratrol synthase gene, a transketolase 1 gene, a fusion gene of a histidine phosphate aminotransferase gene and a tyrosine ammonia lyase gene, a glycolytic pathway hexokinase gene, a 6-phosphofructokinase-1 gene and a phosphoglycerate kinase gene are integrated, so that the recombinant protein is obtained. According to the present invention, by using glucose as the substrate, the high yield of resveratrol can be achieved by using glucose as the substrate, the resveratrol yield during shake-flask culture fermentation can achieve 3.157 g / L, the resveratrol yield can be increased to 30.7 g / L during 5L-scale fed-batch fermentation, and the resveratrol yield can be increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L; the maximum value is reported by the yarrowia lipolytica system at present.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of tyrosol, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain, and the recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain. The recombinant yarrowia lipolytica disclosed by the invention expresses an ethanol dehydrogenase encoding gene, and simultaneously expresses any one or a combination of more of a DAHP synthase mutant encoding gene, a chorismate mutase mutant encoding gene, a glucose 6-phosphate dehydrogenase encoding gene, a transketolase encoding gene and a transaldolase encoding gene; meanwhile, a natural promoter of a pyruvate kinase coding gene is truncated. After the recombinant yarrowia lipolytica XJ-TL strain is subjected to fed-batch fermentation, tyrosol can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of tyrosol can be achieved.

This invention provides a genetically engineered bacterial strain, its preparation method, and its application in the synthesis of perillaldehyde. The genetically engineered bacterial strain includes the CcLPPS gene derived from *Cistus creticus*; the strain is selected from *Yarrowia lipolytica*. This invention constructs a stable, highly efficient de novo perillaldehyde synthesis strain. The entire fermentation process requires no addition of any organic reagents or intermediate substances, significantly reducing production costs and simplifying subsequent separation and purification steps. The engineered *Yarrowia lipolytica* strain provided by this invention exhibits high production intensity and a simple fermentation process, showing promising application prospects and laying the foundation for the microbial fermentation production of perillaldehyde.

The invention belongs to the technical field of bioengineering, and relates to a yarrowia lipolytica strain with high squalene yield, a construction method of the yarrowia lipolytica strain and an application method of the yarrowia lipolytica strain in squalane synthesis. Specifically, a DGA1 gene is knocked out on the basis of constructed recombinant yarrowia lipolytica by utilizing a gene editing means, and then a squalene synthetase gene is integrated on a genome, so that a series of recombinant yarrowia lipolytica strains with high squalene yield are constructed; the high-yield strain is subjected to fermentation culture optimization, tricarboxylic acid circulation metabolism is strengthened, alkane is added into a culture solution for extraction to promote discharge of products, and the final squalene yield reaches 42.1 g / L; the squalene extracted and separated from the fermentation broth is subjected to hydrogenation reduction by taking Reshaki nickel as a catalyst to obtain squalene. The constructed recombinant yarrowia lipolytica strain has the advantages of high yield, simple product extraction, separation and conversion, low production cost, and good industrial application prospects.

The invention provides a method for biosynthesizing menadione by using yarrowia lipolytica. The method is realized by constructing recombinant yarrowia lipolytica, and the construction method of the recombinant yarrowia lipolytica for synthesizing menadione comprises the following steps: intensively expressing a mevalonic acid pathway in the yarrowia lipolytica, and synthesizing menadione by introducing a synthesis pathway of heterologous geranyl and geranyl pyrophosphate. A sufficient isoprene side chain donor is provided for biosynthesis of menadione; on the basis, an aromatic isopentenyl transferase coding gene which is optimized by codons and has menadione substrate specificity is over-expressed in yarrowia lipolytica. The construction method of the yarrowia lipolytica for biosynthesis of menadione provided by the invention is simple to operate, the constructed yarrowia lipolytica can obtain a menadione product with relatively high concentration by taking commercially available menadione as a substrate, the biosynthesis steps are relatively short, and the yarrowia lipolytica has relatively high production and application values.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The invention discloses a method for improving synthesis of sakuranetin from yarrowia lipolytica based on transcriptome analysis and application of the method, and belongs to the technical field of gene engineering. Target genes are analyzed and screened through transcriptome sequencing so as to find a method capable of improving sakuranetin synthesis, and the method specifically comprises the steps that an Illumina HiseqTM platform is used for conducting comprehensive transcriptome analysis on an original strain in the logarithmic phase by applying an RNA-seq technology, then transcriptome data are deeply analyzed through KEGG pathway analysis, and the sakuranetin synthesis can be improved. Therefore, the gene showing significant differential expression in the key path of sakuranetin synthesis is identified. And finally, carrying out free expression and integrated expression on the screened key target gene, and verifying the influence on the sakuranetin yield. Finally, a recombinant yarrowia lipolytica strain which is integrated with the YALI0C01859g gene and has high sakuranetin yield is obtained, and the titer of sakuranetin is increased to 344.0 mg / L.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention relates to the technical field of resource utilization of traditional Chinese medicine wastes, in particular to a fermentation method of traditional Chinese medicine residues. The method comprises the following steps: ultrasonically crushing the traditional Chinese medicine residue to obtain a crushed material, carrying out microwave vacuum drying on the crushed material to obtain a dried material, carrying out low-temperature plasma sterilization on the dried material to obtain a sterilized material, mixing the sterilized material with trichoderma reesei, carrying out aerobic fermentation to obtain an aerobic fermented material, mixing the aerobic fermented material with yarrowia lipolytica, and carrying out anaerobic fermentation. By adopting the method provided by the invention, the flavone retention rate of the traditional Chinese medicine residues is increased, and meanwhile, the grease yield is also increased.

The invention discloses recombinant yarrowia lipolytica with high yield of 3-fucosyllactose as well as a construction method and application of the recombinant yarrowia lipolytica. According to the invention, a reinforced lactose transporter lac12 gene, a reinforced GDP-mannose-4, 6-dehydratase gmd gene, a reinforced GDP-L-fucose synthase gmer gene and a 3-fucosyltransferase SUMO-fut3Bc gene with a reinforced SUMO tag are subjected to integrated expression on a recombinant yarrowia lipolytica chromosome, and a 6-phosphogluconolactonase pgl gene is subjected to overexpression, so that a recombinant yarrowia lipolytica strain is obtained. The constructed recombinant yarrowia lipolytica can maintain the genetic stability of a target gene, the yield and production efficiency of 3-fucosyllactose are remarkably improved, and the requirements of industrial production can be met.

The invention relates to the technical field of genetic engineering, in particular to application of a knockout Fum gene in increasing the yield of erythritol of a strain. The invention provides application of knockout of a Fum gene in a strain in increasing the erythritol yield of the strain, the amino acid sequence of protein coded by the Fum gene is shown as SEQ ID NO: 1, and the strain comprises yarrowia lipolytica. A Fum gene for coding fumarase in a yarrowia lipolytica genome is knocked out by utilizing a CRISPR (clustered regularly interspaced short palindromic repeats) technology, a recombinant yarrowia lipolytica CICC1457-delta Fum with obviously improved erythritol yield is obtained, the erythritol yield is improved by 75.7% and reaches 4.71 g / L compared with that of the original yarrowia lipolytica CICC1457, and in the fermentation culture process, the yield is increased by 75.7% and reaches 4.71 g / L; the thallus growth speed and thallus biomass are not obviously different from those of an original strain.

The invention discloses a high-industrial-property yarrowia lipolytica strain and application thereof, and belongs to the technical field of microbial engineering and fermentation. The mutant is derived from a natural bacterial strain of fermented vegetables, is obtained through high-throughput cell screening, has the advantages and characteristics of high salt resistance, low pH resistance, efficient utilization of an acetic acid substrate, high protein content, no need of vitamins for growth and the like, and is suitable for industrial production of microorganisms; the method can support efficient production of bio-manufactured products under strict conditions of acetic acid carbon source, no sterilization, no seawater, no vitamin and the like.

The present invention belongs to the technical field of microbial synthesis of astaxanthin, and discloses an astaxanthin-producing Yarrowia lipolytica engineered strain, a construction method and application thereof, and a method for preparing astaxanthin. The Yarrowia lipolytica engineered strain provided by the present invention overexpresses peroxisomal membrane protein PEX11 and peroxisomal targeting signal type I receptor PEX5, and can simultaneously synthesize and express β-carotene hydroxylase and β-carotene ketolase. The Yarrowia lipolytica engineered strain has excellent astaxanthin synthesis ability and astaxanthin cell volume, can achieve high-yield synthesis of astaxanthin, provides a basis for large-scale industrial production of astaxanthin, and has good application prospects.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The invention belongs to the technical field of genetic engineering, and particularly relates to application of a Fads gene in regulating and controlling the yield of erythritol produced by fermenting yarrowia lipolytica. The amino acid sequence of the protein coded by the Fads gene is as shown in SEQ ID NO.1, and the yield of the erythritol in the yeast can be remarkably increased by knocking out or knocking down the Fads gene. The result of the embodiment shows that compared with the original strain CICC1457, the growth speed of the Yarrowia lipolytica engineering strain 1k-CICC1457 with the Fads gene knocked out, which is constructed by the invention, is not changed; in addition, the total erythritol yield of the Yarrowia lipolytica engineering strain 1k-CICC1457 strain is obviously increased, and is increased by 1.67 times compared with that of the original strain.

Disclosed in the present invention is a genetically-engineered Yarrowia lipolytica strain highly producing carotenoids, which is obtained by constructing a xylose-induced activation system in the genetically-engineered Yarrowia lipolytica strain XK17 to control the induced expression of critical enzymes for synthesis of astaxanthin, wherein the critical enzymes for synthesis of astaxanthin comprise β-carotene hydroxylase CrtZ and β-carotene ketonase CrtW. Further disclosed in the present invention is the use of the genetically-engineered Yarrowia lipolytica strain in fermentation production of carotenoids. The present invention starts from the Yarrowia lipolytica strain XK17 highly producing β-carotene, and uses the xylose-induced activation system to separately control the expressions of the β-carotene hydroxylase (CrtZ) and β-carotene ketonase (CrtW) or only control the expression of the CrtW or the CrtZ, thereby separately achieving highly producing astaxanthin, or canthaxanthin, or zeaxanthin under the functions of a proper xylose induction concentration and induction opportunity.