254 results about "Yarrowia sp." patented technology

The invention discloses a mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as a construction method and application thereof, the recombinant strain exogenously expresses mesophilic original fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from phaffia rhodozyma, and efficient synthesis under the condition of 30 DEG C is realized; secondly, a metabolic pathway of the recombinant strain is optimized based on a metabolic flow push-pull strategy, and isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), plant alkene dehydrogenase (CarB) and bifunctional plant alkene synthase (CarRP) are overexpressed, so that the flux of a precursor is increased; finally, gene multi-copy of astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from phaffia rhodozyma is carried out, and the carotenoid production capacity of yarrowia lipolytica is further improved. The construction method of the mesophilic yarrowia lipolytica is simple, efficient synthesis of carotenoid can be better achieved at the higher temperature, the fermentation cost is greatly reduced, and the industrial production potential is achieved.

The invention relates to the technical field of microbial fermentation, in particular to a Jianliermondii YT-60 strain and application of the Jianliermondii YT-60 strain in lemon wine, the Jianliermondii YT-60 strain is preserved in Guangdong Microbial Culture Collection Center on April 3, 2025, the preservation number is GDMCC No: 66091, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province, and the preservation number is CGMCC No: 66091. The preservation address of Guangdong Institute of Microbiology is 510070. The invention provides a novel fruit wine fermentation strain, the strain has better tolerance to pH, sugar degree, ethanol and SO2, and the total ester yield is as high as 2.60 g / L. The lemon wine fermented by combining the strain provided by the invention with saccharomyces cerevisiae has more unique flavor than the lemon wine fermented by single saccharomyces cerevisiae.

The invention relates to the technical field of functional lipid preparation, in particular to high-purity nannochloropsis oculata polar lipid enriched by an enzymic method and a preparation method of the high-purity nannochloropsis oculata polar lipid. The method comprises the following steps: specifically and selectively hydrolyzing triglyceride by using immobilized Lip2 lipase to generate glycerol and free fatty acid, retaining and enriching polar lipid components, and carrying out centrifugal filtration and molecular distillation to obtain the high-purity nannochloropsis oculata polar lipid. The invention provides a method for preparing high-purity polar lipid by performing enzymolysis on nannochloropsis oculata oil by using lipase Lip2 produced by yarrowia lipolytica, which maximally retains the polar lipid and obtains a high-purity polar lipid product through the specific catalytic action of Lip2 lipase. The method which is mild in reaction condition, environment-friendly, simple in step and high in purity is provided for efficient enrichment of the nannochloropsis oculata polar lipid, the prepared polar lipid product is extremely high in purity, and the blank of efficient preparation of the nannochloropsis oculata polar lipid in the prior art is filled.

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention belongs to the technical field of biology, and discloses an exogenous cofactor enhanced yarrowia lipolytica strain, a method and application in efficient synthesis of EPA (eicosapentaenoic acid) and lipids.The chassis strain of the yarrowia lipolytica strain is yarrowia lipolytica Po1f, desaturase is partially prolonged in an overexpression fatty acid synthesis route in the strain, and the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids. The desaturase gene mainly comprises a delta 9 elongase gene, a delta 8 desaturase gene, a delta 5 desaturase gene and a delta 17 desaturase gene, and G6PDH and malic enzyme are overexpressed at the same time on the basis. The strain provided by the invention improves the EPA and lipid yield of yarrowia lipolytica; the method is suitable for constructing a genetic engineering strain with high yield of EPA and lipid, provides a basis for producing grease rich in EPA from yarrowia lipolytica, and solves the problems of non-sustainability, high cost and the like caused by obtaining EPA from fish.

The invention belongs to the field of polypeptides, and particularly relates to an antibacterial peptide derived from marine microorganisms, an antibacterial composition and application. Based on a deep learning algorithm, five potential antibacterial peptides are mined from a global marine microbiome database, and the five antibacterial peptides are expressed in yarrowia lipolytica. Experimental tests show that the five antibacterial peptides have differentiated antibacterial effects on different bacteria, and the antibacterial peptide shown in SEQ ID NO: 3 can have an obvious inhibition effect on escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, has low hemolysis and has a positive application prospect. The antibacterial peptide disclosed by the invention has the potential of further developing novel antibacterial drugs or antibacterial additives.

The invention discloses a high-yield delta-tocotrienol genetically engineered bacterium and application thereof, and belongs to the technical field of gene recombination. The invention discloses a genetically engineered bacterium for high yield of delta-tocotrienols, which is characterized in that yarrowia lipolytica ATCC MYA-2613 is used as an original strain, and a ku70 gene is knocked out; the gene is used for expressing and coding 4-hydroxyphenylpyruvate dioxygenase, the gene is used for coding uromelanic acid phytate transferase and the gene is used for coding tocopherol cyclase; intensifying a shikimic acid pathway; a mevalonic acid pathway is strengthened; an isopentenol path is introduced; carrying out protein fusion on the HPD and the HPT; truncating a signal peptide of a gene encoding a tocopherol cyclase and back-filling a leucine defect. The genetically engineered bacterium constructed by the invention has industrial stability, can be amplified by more than 10 times in a 2L fermentation tank level compared with a shake flask level, can produce 616.45 mg / L of delta-tocotrienol after being cultured for 240 hours, and has industrial application prospects.

The invention discloses a yarrowia lipolytica engineering strain with high yield of resveratrol as well as construction and application of the yarrowia lipolytica engineering strain, and belongs to the technical field of synthetic biology and metabolic engineering. The invention provides a yarrowia lipolytica engineering strain. A 4-coumaric acid-CoA ligase gene, a multi-copy resveratrol synthase gene, a transketolase 1 gene, a fusion gene of a histidine phosphate aminotransferase gene and a tyrosine ammonia lyase gene, a glycolytic pathway hexokinase gene, a 6-phosphofructokinase-1 gene and a phosphoglycerate kinase gene are integrated, so that the recombinant protein is obtained. According to the present invention, by using glucose as the substrate, the high yield of resveratrol can be achieved by using glucose as the substrate, the resveratrol yield during shake-flask culture fermentation can achieve 3.157 g / L, the resveratrol yield can be increased to 30.7 g / L during 5L-scale fed-batch fermentation, and the resveratrol yield can be increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L; the maximum value is reported by the yarrowia lipolytica system at present.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of tyrosol, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain, and the recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain. The recombinant yarrowia lipolytica disclosed by the invention expresses an ethanol dehydrogenase encoding gene, and simultaneously expresses any one or a combination of more of a DAHP synthase mutant encoding gene, a chorismate mutase mutant encoding gene, a glucose 6-phosphate dehydrogenase encoding gene, a transketolase encoding gene and a transaldolase encoding gene; meanwhile, a natural promoter of a pyruvate kinase coding gene is truncated. After the recombinant yarrowia lipolytica XJ-TL strain is subjected to fed-batch fermentation, tyrosol can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of tyrosol can be achieved.

This invention provides a genetically engineered bacterial strain, its preparation method, and its application in the synthesis of perillaldehyde. The genetically engineered bacterial strain includes the CcLPPS gene derived from *Cistus creticus*; the strain is selected from *Yarrowia lipolytica*. This invention constructs a stable, highly efficient de novo perillaldehyde synthesis strain. The entire fermentation process requires no addition of any organic reagents or intermediate substances, significantly reducing production costs and simplifying subsequent separation and purification steps. The engineered *Yarrowia lipolytica* strain provided by this invention exhibits high production intensity and a simple fermentation process, showing promising application prospects and laying the foundation for the microbial fermentation production of perillaldehyde.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention relates to the technical field of resource utilization of traditional Chinese medicine wastes, in particular to a fermentation method of traditional Chinese medicine residues. The method comprises the following steps: ultrasonically crushing the traditional Chinese medicine residue to obtain a crushed material, carrying out microwave vacuum drying on the crushed material to obtain a dried material, carrying out low-temperature plasma sterilization on the dried material to obtain a sterilized material, mixing the sterilized material with trichoderma reesei, carrying out aerobic fermentation to obtain an aerobic fermented material, mixing the aerobic fermented material with yarrowia lipolytica, and carrying out anaerobic fermentation. By adopting the method provided by the invention, the flavone retention rate of the traditional Chinese medicine residues is increased, and meanwhile, the grease yield is also increased.

The invention discloses recombinant yarrowia lipolytica with high yield of 3-fucosyllactose as well as a construction method and application of the recombinant yarrowia lipolytica. According to the invention, a reinforced lactose transporter lac12 gene, a reinforced GDP-mannose-4, 6-dehydratase gmd gene, a reinforced GDP-L-fucose synthase gmer gene and a 3-fucosyltransferase SUMO-fut3Bc gene with a reinforced SUMO tag are subjected to integrated expression on a recombinant yarrowia lipolytica chromosome, and a 6-phosphogluconolactonase pgl gene is subjected to overexpression, so that a recombinant yarrowia lipolytica strain is obtained. The constructed recombinant yarrowia lipolytica can maintain the genetic stability of a target gene, the yield and production efficiency of 3-fucosyllactose are remarkably improved, and the requirements of industrial production can be met.

The invention discloses a high-industrial-property yarrowia lipolytica strain and application thereof, and belongs to the technical field of microbial engineering and fermentation. The mutant is derived from a natural bacterial strain of fermented vegetables, is obtained through high-throughput cell screening, has the advantages and characteristics of high salt resistance, low pH resistance, efficient utilization of an acetic acid substrate, high protein content, no need of vitamins for growth and the like, and is suitable for industrial production of microorganisms; the method can support efficient production of bio-manufactured products under strict conditions of acetic acid carbon source, no sterilization, no seawater, no vitamin and the like.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

Disclosed in the present invention is a genetically-engineered Yarrowia lipolytica strain highly producing carotenoids, which is obtained by constructing a xylose-induced activation system in the genetically-engineered Yarrowia lipolytica strain XK17 to control the induced expression of critical enzymes for synthesis of astaxanthin, wherein the critical enzymes for synthesis of astaxanthin comprise β-carotene hydroxylase CrtZ and β-carotene ketonase CrtW. Further disclosed in the present invention is the use of the genetically-engineered Yarrowia lipolytica strain in fermentation production of carotenoids. The present invention starts from the Yarrowia lipolytica strain XK17 highly producing β-carotene, and uses the xylose-induced activation system to separately control the expressions of the β-carotene hydroxylase (CrtZ) and β-carotene ketonase (CrtW) or only control the expression of the CrtW or the CrtZ, thereby separately achieving highly producing astaxanthin, or canthaxanthin, or zeaxanthin under the functions of a proper xylose induction concentration and induction opportunity.

The invention belongs to the technical field of biological engineering, and discloses a schizochytrium limacinum engineering strain co-expressing a PDC-ACC gene, a construction method and application, and the genetic engineering strain is obtained by taking schizochytrium limacinum as an original strain and expressing a pyruvate decarboxylase compound gene PDC and an acetyl-coenzyme A carboxylase gene ACC in yarrowia lipolytica. The PDC and ACC genes in the schizochytrium limacinum genetic engineering strain disclosed by the invention are cloned to yarrowia lipolytica, namely Po1f. The yield of traditional fermented grease is 43.90 g / L. The engineering strain performs overexpression on PDC and ACC genes, so that the grease content of the schizochytrium limacinum engineering strain is increased by 53.13% and reaches 67.22 g / L, and the DHA content is increased by 36.97% and is increased from 40.71% to 55.76%.

The invention relates to the technical field of genetic engineering, in particular to recombinant yarrowia lipolytica with high yield of sclareol as well as a preparation method and application of the recombinant yarrowia lipolytica. By analyzing the metabolism principle of yarrowia lipolytica, a key metabolic pathway for producing sclareol is regulated and controlled, eight recombinant strains are designed and constructed, CJ-8 overexpresses a tPaGGPPS gene, a four-copy SsSL gene, an ERG19 gene, an ERG13 gene, an ERG12 gene, an ERG8 gene and a CAT2 gene, and a DGA2 gene is knocked out at the same time. Compared with an initial strain and other seven recombinant strains, the CJ-8 can be fermented to synthesize sclareol, the synthesis capability is remarkably improved, and the sclareol yield of the CJ-8 reaches 2747.50 mg / L. In conclusion, when the recombinant yarrowia lipolytica provided by the invention is used for fermentation production of sclareol, the yield of sclareol can be greatly improved.

The invention relates to a beta-carotene production engineering bacterium as well as a construction method and application thereof. According to the engineering bacterium, Yarrowia lipolytica is taken as an original strain, and a beta-carotene synthetic pathway related gene is integrated in a genome of the Yarrowia lipolytica; wherein the beta-carotene synthetic pathway related genes comprise MvaS, MvaE, ERG12, ERG8, ERG19, IDI, ERG20, GGPPs7, CarRP and CarB, and the beta-carotene synthetic pathway related genes comprise beta-carotene, beta-carotene, beta-carotene, beta-carotene, beta-carotene, beta-carotene, beta-carotene and beta-carotene. The beta-carotene synthetic pathway related gene is subjected to localization expression in peroxisome through the peroxisome localization signal peptide. The engineering strain provided by the invention can obviously improve the yield of beta-carotene.

The invention relates to a yarrowia lipolytica strain for producing tyrosol as well as a construction method and application of the yarrowia lipolytica strain, and belongs to the technical field of genetic engineering. According to the present invention, ScADH6, ScARO10F138L and D218G genes are expressed in the yarrowia lipolytica W29 [delta] KU70; the shikimic acid feedback inhibition is relieved by expressing the Y1ARO3K225L gene, the Y1ARO4K2214 gene and the Y1AR07G139S gene; pmLAAD, EcTyrAM53I and A354V genes are introduced, so that the conversion from FAD to FADH2 is promoted, and the supply of NADH (Nicotinamide Adenine Dinucleotide Hormone) in cytoplasm is enhanced; a 4-hydroxyphenylpyruvate dioxygenase (HPD) gene is knocked out; and overexpressing Y1DHS1, Y1DHS2 and Y1DHS3 genes of a shikimic acid pathway to obtain the yarrowia lipolytica strain HY5 for producing tyrosol. The invention lays a foundation for industrial production of tyrosol, and has important social significance and economic value.

The invention belongs to the technical field of bioengineering, and relates to a construction method and application of recombinant yarrowia lipolytica for synthesizing plant antitoxin Kauralexin A1. The method is realized by constructing recombinant yarrowia lipolytica. The construction method of the recombinant yarrowia lipolytica for synthesizing the Kauralexin A1 comprises the following steps: overexpressing a codon optimized antitoxin Kauralexin A1 biosynthetic pathway in yarrowia lipolytica, screening adaptive P450 reductase to improve the electron transfer efficiency in a P450 system, and constructing a metabolic pathway scaffold-free multi-enzyme complex to enhance cascade biological catalysis and metabolic flux, so as to obtain the recombinant yarrowia lipolytica for synthesizing the Kauralexin A1. The expression of the mevalonic acid pathway is enhanced, so that a sufficient GGPP precursor is provided for the biosynthesis of the Kauralexin A1. The construction method of the yarrowia lipolytica for biosynthesizing the antitoxin Kauralexin A1, provided by the invention, is simple to operate, and the constructed yarrowia lipolytica can be used for efficiently producing the Kauralexin A1 and has relatively high production and application values.

The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.

The invention discloses an erythritol reductase mutant and application thereof, and belongs to the technical field of variation or genetic engineering. In particular discloses an erythritol reductase mutant with at least one of the following mutations relative to an amino acid sequence shown as SEQ ID NO: 1: K42R, I49A, I49V, F75S, L107Y, Y125A, Y125L and I296A, and application of the erythritol reductase mutant in increasing the yield of erythritol. The mutant capable of promoting synthesis of erythritol is obtained through three-dimensional modeling and key catalytic pocket searching, and the yield of the erythritol of the yarrowia lipolytica is remarkably increased by using the genetic engineering strain constructed by the mutant and can be increased by 77% to the maximum. The erythritol synthesis capability of the erythritol reductase mutant is greatly improved, the erythritol reductase mutant is more suitable for industrial application, the production cost can be remarkably reduced, and the production efficiency is improved.

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The application provides a recombinant Yarrowia lipolytica capable of producing palmitoleic acid at a high yield, a construction method and application thereof, and belongs to the technical field of bioengineering. Yarrowia lipolytica The recombinant Yarrowia lipolytica is obtained by knocking out peroxisome biogenesis factor 10 and triacylglycerol lipase 4 in the genome of Yarrowia lipolytica and inserting an acetyl-CoA carboxylase, a Δ9 desaturase, an acetyl-CoA diacylglycerol acyltransferase, a glycerol-3-phosphate acyltransferase, a lysophosphatidylcholine acyltransferase and a phosphatidylcholine:diacylglycerol acyltransferase gene expression cassette. Experiment proves that the recombinant Yarrowia lipolytica can efficiently ferment and produce palmitoleic acid, and realizes efficient synthesis of the natural product palmitoleic acid from plants in Yarrowia lipolytica.

The embodiment of the invention discloses yarrowia caribeckii as well as a separation and screening method, an identification method and application of the yarrowia caribeckii. The preservation number of the yarrowia caribica is CGMCC (China General Microbiological Culture Collection Center) No.27604, and the preservation number of the yarrowia caribica is CGMCC No.27604. The Caribik Yarrowia spp. Provided by the embodiment of the invention has a good antagonistic effect on gray mold, penicilliosis, black spot and aspergillus niger after fruits are picked, and can obviously reduce the disease morbidity of the fruits of strawberries, apples, tomatoes and pears. And the antagonistic bacterium is identified to be Meyerozyma caribbica, and the antagonistic bacterium can be used as the Meyerozyma caribbica. By respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris, the disease prevention and control effects of Meyerozyma caribbica can be enhanced. The disease prevention and control effects of the Meyerozyma caribbica can be enhanced by adding the auxiliary factors of calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The invention provides a recombinant Yarrowia lipolytica. The recombinant Yarrowia lipolytica comprises a heterologous polynucleotide encoding a fusion protein. The fusion protein comprises a first amino acid sequence and a second amino acid sequence. The first amino acid may be homologous to the amino acid sequence of 3-ketoacyl CoA thiolase (3KAT), The second amino acid sequence may be homologous to the amino acid sequence of a fatty acyl-CoA reductase (FAR). Also provided is a method for producing one or more fatty alcohols by the recombinant Yarrowia lipolytica and a method for preparing the recombinant Yarrowia lipolytica.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing zeaxanthin as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, the yarrowia lipolytica is subjected to genetic engineering modification based on a genetic engineering technology, a zeaxanthin biosynthetic pathway is constructed by expressing beta-carotene hydroxylase in an original strain, and meanwhile, a zeaxanthin biosynthetic pathway is constructed through a multiple metabolic engineering modification strategy. The method comprises the steps of key enzyme PaCrtZ multi-copy integration, oxidation-reduction balance regulation and control, upstream mevalonic acid pathway enhancement through membrane-free organelles and the like, and the yield of zeaxanthin is further increased. Meanwhile, by optimizing the culture conditions and applying the optimal fermentation conditions to perform tank fermentation, the yarrowia lipolytica can continuously generate more zeaxanthin, so that the yarrowia lipolytica has excellent practical application value and application prospect.