153 results about "Yarrowia sp." patented technology

The invention relates to the technical field of functional lipid preparation, in particular to high-purity nannochloropsis oculata polar lipid enriched by an enzymic method and a preparation method of the high-purity nannochloropsis oculata polar lipid. The method comprises the following steps: specifically and selectively hydrolyzing triglyceride by using immobilized Lip2 lipase to generate glycerol and free fatty acid, retaining and enriching polar lipid components, and carrying out centrifugal filtration and molecular distillation to obtain the high-purity nannochloropsis oculata polar lipid. The invention provides a method for preparing high-purity polar lipid by performing enzymolysis on nannochloropsis oculata oil by using lipase Lip2 produced by yarrowia lipolytica, which maximally retains the polar lipid and obtains a high-purity polar lipid product through the specific catalytic action of Lip2 lipase. The method which is mild in reaction condition, environment-friendly, simple in step and high in purity is provided for efficient enrichment of the nannochloropsis oculata polar lipid, the prepared polar lipid product is extremely high in purity, and the blank of efficient preparation of the nannochloropsis oculata polar lipid in the prior art is filled.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention discloses a high-industrial-property yarrowia lipolytica strain and application thereof, and belongs to the technical field of microbial engineering and fermentation. The mutant is derived from a natural bacterial strain of fermented vegetables, is obtained through high-throughput cell screening, has the advantages and characteristics of high salt resistance, low pH resistance, efficient utilization of an acetic acid substrate, high protein content, no need of vitamins for growth and the like, and is suitable for industrial production of microorganisms; the method can support efficient production of bio-manufactured products under strict conditions of acetic acid carbon source, no sterilization, no seawater, no vitamin and the like.

The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The application provides a recombinant Yarrowia lipolytica capable of producing palmitoleic acid at a high yield, a construction method and application thereof, and belongs to the technical field of bioengineering. Yarrowia lipolytica The recombinant Yarrowia lipolytica is obtained by knocking out peroxisome biogenesis factor 10 and triacylglycerol lipase 4 in the genome of Yarrowia lipolytica and inserting an acetyl-CoA carboxylase, a Δ9 desaturase, an acetyl-CoA diacylglycerol acyltransferase, a glycerol-3-phosphate acyltransferase, a lysophosphatidylcholine acyltransferase and a phosphatidylcholine:diacylglycerol acyltransferase gene expression cassette. Experiment proves that the recombinant Yarrowia lipolytica can efficiently ferment and produce palmitoleic acid, and realizes efficient synthesis of the natural product palmitoleic acid from plants in Yarrowia lipolytica.

The embodiment of the invention discloses yarrowia caribeckii as well as a separation and screening method, an identification method and application of the yarrowia caribeckii. The preservation number of the yarrowia caribica is CGMCC (China General Microbiological Culture Collection Center) No.27604, and the preservation number of the yarrowia caribica is CGMCC No.27604. The Caribik Yarrowia spp. Provided by the embodiment of the invention has a good antagonistic effect on gray mold, penicilliosis, black spot and aspergillus niger after fruits are picked, and can obviously reduce the disease morbidity of the fruits of strawberries, apples, tomatoes and pears. And the antagonistic bacterium is identified to be Meyerozyma caribbica, and the antagonistic bacterium can be used as the Meyerozyma caribbica. By respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris, the disease prevention and control effects of Meyerozyma caribbica can be enhanced. The disease prevention and control effects of the Meyerozyma caribbica can be enhanced by adding the auxiliary factors of calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The invention provides a recombinant Yarrowia lipolytica. The recombinant Yarrowia lipolytica comprises a heterologous polynucleotide encoding a fusion protein. The fusion protein comprises a first amino acid sequence and a second amino acid sequence. The first amino acid may be homologous to the amino acid sequence of 3-ketoacyl CoA thiolase (3KAT), The second amino acid sequence may be homologous to the amino acid sequence of a fatty acyl-CoA reductase (FAR). Also provided is a method for producing one or more fatty alcohols by the recombinant Yarrowia lipolytica and a method for preparing the recombinant Yarrowia lipolytica.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing zeaxanthin as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, the yarrowia lipolytica is subjected to genetic engineering modification based on a genetic engineering technology, a zeaxanthin biosynthetic pathway is constructed by expressing beta-carotene hydroxylase in an original strain, and meanwhile, a zeaxanthin biosynthetic pathway is constructed through a multiple metabolic engineering modification strategy. The method comprises the steps of key enzyme PaCrtZ multi-copy integration, oxidation-reduction balance regulation and control, upstream mevalonic acid pathway enhancement through membrane-free organelles and the like, and the yield of zeaxanthin is further increased. Meanwhile, by optimizing the culture conditions and applying the optimal fermentation conditions to perform tank fermentation, the yarrowia lipolytica can continuously generate more zeaxanthin, so that the yarrowia lipolytica has excellent practical application value and application prospect.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

The present application relates to the field of biosynthesis and microbial fermentation engineering technology, in particular to a kind of secretion expression human lactoferrin Yarrowia lipolytica and its construction method and application.The present application provides a kind of secretion expression human lactoferrin recombinant Yarrowia lipolytica, the secretion expression of human lactoferrin in the recombinant Yarrowia lipolytica is guided by secretion signal peptide;The expression of chaperone HAC1, Cne and Pdi1 is simultaneously enhanced.The present application realizes the secretion expression of human lactoferrin in Yarrowia lipolytica, effectively promotes the folding and secretion expression of human lactoferrin by the cooperation of chaperone, significantly improves the yield of human lactoferrin, and has important application value in the industrial scale production of human lactoferrin.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.

The application belongs to the technical field of biology, and discloses a Yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application, wherein a chassis strain of the engineering strain is Yarrowia lipolytica Po1f, and genes of Δ9 elongase, Δ8 desaturase, Δ5 desaturase and Δ17 desaturase in a fatty acid synthesis pathway, a heterologous ATP-citrate lyase and a mitochondrial citrate carrier are overexpressed. ATP-citrate lyases and mitochondrial citrate carrier genes respectively derived from Schizochytrium sp. and Saccharomyces cerevisiae are combined to be expressed in the Yarrowia lipolytica engineering strain, so that the production of EPA and oil in the Yarrowia lipolytica engineering strain is significantly improved, and compared with a control strain, the production of oil and EPA is respectively increased by 243.35% and 161.85%.

The invention discloses yarrowia lipolytica and application thereof, and belongs to the technical field of microorganisms. According to the present invention, the Yarrowia lipolytica H74 strain is separated, and the preservation number of the Yarrowia lipolytica H74 strain is CGMCC (China General Microbiological Culture Collection Center) 34876; after the H74 strain is co-fermented with the figs, the aroma quality of the figs can be effectively improved. The fermentation liquor after co-fermentation is added into a cigarette product for verification, and the sensory evaluation result of the cigarette can be remarkably improved. After the H74 strain is applied to the field of cigarette perfuming, not only can the richness, the stability and the coordination of fig fragrance be improved, but also the use of chemical synthetic spices can be reduced, the development trend of naturalness, harm reduction and characterization in the tobacco industry is met, and scientific and technological innovation and industrial application of biological fragrance production are accelerated and promoted.

The invention discloses a method for promoting yarrowia lipolytica to synthesize carotenoid, which comprises the following steps of: recombining and expressing beta-carotene hydroxylase CrtZ and / or beta-carotene ketolase CrtW fused with a short peptide label in the yarrowia lipolytica producing beta-carotene; site-specific mutagenesis is carried out on the amino acid site of CrtZ; and a CrtZ coding gene is integrated in a multi-copy manner. The invention also discloses a yarrowia lipolytica genetically engineered bacterium for producing beta-carotene, wherein carotenoid is selected from zeaxanthin, canthaxanthin and astaxanthin. The yield of zeaxanthin, canthaxanthin and astaxanthin in yarrowia lipolytica is remarkably increased through enzyme label engineering, combinatorial optimization and site-specific mutagenesis strategies, and a new path is provided for efficient synthesis of carotenoids.

The invention provides a method for preparing erythritol and application thereof, and belongs to the technical field of erythritol production. The method for preparing the erythritol provided by the invention comprises the following steps: inoculating yarrowia lipolytica into a fermentation culture medium, and fermenting to obtain the erythritol, the fermentation culture medium is prepared from glucose, yeast powder, KH2PO4, NaCl, CaCl2, vitamin B1 and water. According to the method for preparing the erythritol, high-efficiency production of the erythritol is carried out by regulating and controlling the key enzyme of the bypass metabolic pathway of the hexose phosphate, the yield of the erythritol can be remarkably increased, the conversion rate of the erythritol reaches 60% or above, and the method has the advantages that the process control is simple, the fermentation time is short, and the yield of the erythritol is high.

The application relates to the technical field of genetically modified yeasts, in particular to a Yarrowia lipolytica with high homologous recombination efficiency and / or low lipid degradation capacity and a construction method thereof. The engineered Yarrowia lipolytica comprises a copper ion inducible promoter and a gene related to homologous recombination; the copper ion inducible promoter is selected from any one or more of the following: a pCTR2 promoter, a pMT2 promoter and a pUAS16MT2 constitutive promoter; wherein the gene related to homologous recombination is selected from any one or more of the following: a ylKU70 gene, a ylKU80 gene, a ylRAD51 gene, a ylRAD52 gene, a scRAD52 gene and a scRAD59 gene. The homologous recombination efficiency of the engineered Yarrowia lipolytica is significantly improved. Furthermore, the POX5 gene, the POX2 gene and / or the POX3 gene are knocked out, so that the lipid degradation capacity of the engineered Yarrowia lipolytica is significantly reduced.

The invention discloses application of yarrowia caribbica NJC36 in prevention and treatment of aspergillus flavus, and belongs to the technical field of biological prevention and treatment. Experiments prove that the yarrowia caribbica NJC36 can inhibit the growth of aspergillus flavus and the synthesis of aflatoxin, and can be used for preventing and treating kiwi fruits from being infected by the aspergillus flavus. The yarrowia caribbica NJC36 disclosed by the invention has a remarkable prevention and treatment effect on aspergillus flavus, can inhibit the synthesis of aflatoxin, and is environment-friendly.

The invention discloses application of oxygen-carrying protein VHb in construction of high-yield astaxanthin engineering bacteria, and belongs to the technical field of biology. According to the method, carRPY27R, carB, fusion genes crtW-crtZ and GGPPS are iteratively integrated onto a yarrowia lipolytica chassis genome by utilizing a synthetic biological technology, so that two recombinant strains for producing astaxanthin are obtained. On the basis, the oxygen-carrying protein VHb from vitreoscilla is expressed, and compared with the oxygen-carrying protein VHb which is not expressed, the VHb is positioned to the endoplasmic reticulum, so that the accumulation level of astaxanthin is improved by 272.99%, and the biomass is improved by 94.15%. According to the method, the oxygen-carrying protein VHb is expressed on cytoplasm and organelle, so that the synthesis efficiency and biomass of astaxanthin are remarkably improved. In addition, the intracellular dissolved oxygen level is improved, so that the cost for improving the dissolved oxygen in the fermentation process is reduced.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin based on a fusion protein method and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketoalcohol enzyme CrtW and beta-carotene hydroxylase CrtZ, and astaxanthin synthesis key enzymes CrtW and CrtZ are subjected to fusion expression by using a short peptide or Linker, so that the astaxanthin is obtained. Meanwhile, the expression of hemoglobin VHb from vitreoscilla is increased; the astaxanthin production performance of the recombinant strain is verified on the basis of protein engineering and fermentation engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention discloses a special microbial compound fertilizer for improving quality and enhancing aroma of pandan leaves and a preparation method of the special microbial compound fertilizer, and belongs to the technical field of bacterial fertilizers. The microbial compound fertilizer is obtained by utilizing compound bacteria to ferment and treat waste, the compound bacteria are formed by compounding yarrowia lipolytica, trichoderma and bacillus subtilis, and the waste is formed by compounding pineapple leaves and banana stems. In the compound bacteria, yarrowia lipolytica, trichoderma and bacillus subtilis are compounded in the form of bacterial powder, and the mass ratio is (1-5): (1-2): (1-4). The preservation number of the yarrowia lipolytica is ACCC (China General Microbiological Culture Collection Center) 21176, and the preservation number of the trichoderma sp. Is ACCC 31526; the mass ratio of pineapple leaves to banana stems in the waste is 3: 4. When the microbial fertilizer is applied to pandan leaf planting soil, the aroma components of the pandan leaves can be remarkably improved, and a novel bacterial fertilizer is provided for improving the quality and aroma of the pandan leaves.

The invention relates to the technical field of microorganisms, in particular to recombinant genetically engineered bacteria of yarrowia lipolytica as well as a construction method and application of the recombinant genetically engineered bacteria. The succinic acid production performance of the recombinant genetically engineered bacterium of yarrowia lipolytica is remarkably improved, high succinic acid yield and production intensity are achieved, the recombinant genetically engineered bacterium can be applied to stable and efficient production of succinic acid, the performance of the recombinant genetically engineered bacterium is stable, and the recombinant genetically engineered bacterium can be suitable for large-scale industrial production of succinic acid.

The present disclosure relates to yeasts, in particular Yarrowia cells, with enhanced production of heme. Also provided are methods for enhanced production of heme with Yarrowia cells.

The invention provides a recombinant yarrowia lipolytica strain with high yield of eicosapentaenoic acid as well as a construction method and application of the recombinant yarrowia lipolytica strain, and belongs to the technical field of genetic engineering. The invention provides a recombinant yarrowia lipolytica strain Po1f: Y1 capable of reconstructing an EPA (eicosapentaenoic acid) synthetic route, and the recombinant yarrowia lipolytica strain Po1f: Y2 is constructed by taking the recombinant yarrowia lipolytica strain Po1f: Y1 as a host and overexpressing an LPCAT (Low Performance Protein Amino Transferase) mutant; and knocking out encoding genes such as PEX10, GUT2, TGL4, SNF1 and the like, and combining with overexpressed DGAT1 and the like to obtain a recombinant yarrowia lipolytica strain Po1f: Y3 with high yield of EPA. The recombinant yarrowia lipolytica strain Po1f: Y3 strain EPA accounts for 50% of the relative content of total fatty acid, the oil content accounts for 48% of the weight of stem cells, and the biomass of recombinant yeast in a 7.5 L fermentation tank exceeds 100 g stem cell weight / L.

The invention relates to the technical field of strain breeding, in particular to a high-yield beta-glucan Meyrowia guilliermondii strain and application thereof. According to the present invention, the high-yield beta-glucan mutant strain obtained through ARTP mutagenesis has the name of Meyerozyma guilliermondii NCUASD7, has been preserved in Guangdong Microbial Culture Collection Center on November 28, 2025, and has the preservation number of CDMCC NO: 67365; aRTP mutagenesis is high in mutation rate, good in hereditary stability and safer to operate, a large number of mutation libraries can be obtained within a short time, high-throughput primary screening and shake flask secondary screening are conducted through a 24-deep-hole plate, a yeast mutant strain with the high beta-glucan yield capacity is obtained, then subculture is conducted on the high-yield mutant strain, the hereditary stability of the strain is verified, and the high beta-glucan yield is obtained. The mutant strain not only has the characteristic of high yield of beta-glucan, but also can maintain good genetic stability.

The present application belongs to the field of genetic engineering and microbial fermentation technology, and discloses a kind of construction cell wall formation related gene deletion Yarrowia lipolytica Recombinant strain, the recombinant strain is constructed by genetic engineering means to knock out its cell wall formation related gene.The cell wall structure of the recombinant strain of the present application changes, resulting in reduced cell wall stability, and does not affect intracellular synthesis and accumulation of oil.The fermentation broth of the modified strain is treated by conventional cell wall breaking method, and the cell wall breaking rate is obviously improved, which shows that this technical route can provide a better potential choice for improving the cell wall breaking rate of Yarrowia lipolytica.The construction method of the recombinant strain provided by the present application is simple and easy to use, and can be used as an upstream technical means for biological production of Yarrowia lipolytica.Only one modification can obtain sustained technical effect, to fundamentally solve the technical problems of thick cell wall and difficult to break, low cost, high benefit, and has good application prospect.

The application discloses sulfonated modified metal organic framework material and a preparation method thereof and application of the sulfonated modified metal organic framework material in yield increase of aryl glycoside compounds, wherein the sulfonated modified metal organic framework material is prepared by taking a zirconium-based MOF material Zr-BTB as a precursor and reacting with 1,2 ethanedithoic acid. The prepared sZr-BTB can be fixed on the surface of Yarrowia lipolytica by the principle of physical adsorption, so as to form a dense protective film, so that the relatively large molecular glycosidase is limited in the cell and cannot be secreted, the efflux of the small molecular aryl glycoside compound is not affected, the decomposition of the main biosynthesis product is avoided, meanwhile, the stable adsorption with Yarrowia lipolytica can be maintained under strong acidic conditions, the yield of the fermentation production of the aryl glycoside compound is improved, and the hindering of the non-model microorganism is solved, and the low-cost industrialization of the biosynthesis is promoted.

The invention provides a monoamine oxidase mutant, recombinant yarrowia lipolytica and application of the monoamine oxidase mutant and the recombinant yarrowia lipolytica. Monoamine oxidase is subjected to site-directed mutagenesis modification by searching a key catalytic pocket, so that the catalytic efficiency of monoamine oxidase is improved, and biosynthesis of bovine heart alkali is increased; finally, the Phe208Ala mutant is obtained, and the capacity of synthesizing bovine heart alkali by utilizing a genetic engineering strain constructed by the mutant is obviously improved; an MAO-A wild type and a MAO-A mutant are respectively expressed and designed in engineering bacteria, the ability of the mutant for catalyzing dopamine to generate bovine heart alkali in yarrowia lipolytica is compared, and the result shows that the monoamine oxidase mutant can significantly improve the yield of the yarrowia lipolytica bovine heart alkali, the yield is significantly improved by 40.05% compared with an original strain, and the monoamine oxidase mutant has a good application prospect. The method has better performance, can significantly improve the yield of biosynthesized phylline, is more suitable for industrial application in the future development process, and can significantly reduce the production cost and improve the production efficiency.

The invention discloses a method for promoting yarrowia lipolytica to synthesize carotenoid, which comprises the following steps of: recombining and expressing beta-carotene hydroxylase CrtZ and / or beta-carotene ketolase CrtW fused with a short peptide label in the yarrowia lipolytica for producing beta-carotene; wherein the CrtZ and / or CrtW fused with the oligopeptide tags are / is formed by (1) inserting CrtZ added with different oligopeptide tags into a yarrowia lipolytica genome, (2) inserting CrtW added with different oligopeptide tags into the yarrowia lipolytica genome, or (3) inserting CrtZ added with different oligopeptide tags and CrtW added with different oligopeptide tags into the yarrowia lipolytica genome; and the positions of the CrtZ and the CrtW added with the short peptide tags are N ends or C ends. According to the invention, the yield of carotenoids synthesized by yarrowia lipolytica is improved by recombinant expression of beta-carotene hydroxylase CrtZ and / or beta-carotene ketolase CrtW fused with short peptide tags in yarrowia lipolytica.