The invention particularly relates to yarrowia lipolytica with high yield of [beta]-carotene and application thereof. The strain can utilize common carbon sources and nitrogen sources, after high-density culture, the yield of [beta]-carotene reaches 7.8 g/L, the content reaches 161 mg/g DCW, and the strain is an excellent strain for high yield of [beta]-carotene. According to the engineering strain, a low-yield [beta]-carotene yarrowia lipolytica strain T1 which integrates CarRP and CarB genes to a zeta site at a fixed point is taken as a starting strain, endogenous key enzymes HMGs, HMGCR, ERG20, GGS1, ERG8, ERG12, ERG19 and IDI are subjected to multiple rounds of random integration, and exogenous GGPP synthetase GPS (which can directly catalyze IPP and DMAPP into GGPP) and hemoglobin VHb are introduced; and finally, the key gene AAL8 for reducing lipidosome degradation is knocked out to obtain the high-yield strain. As a [beta]-carotene production engineering bacterium, the strain has a good application prospect in the fields of food, feed, medicine and cosmetics.