Method for producing lipase, transformed Yarrowia lipolytica cell capable of producing lipase and their uses

A technology of Yarrowia lipolytica and production method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inappropriate lipase production, and achieve the effect of promoting application
CN102703404AActive Publication Date: 2012-10-03LABES MAYOLY SPINDLER R L
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
LABES MAYOLY SPINDLER R L
Publication Date
2012-10-03

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Abstract

A method for producing Yarrowia lipolytica acid-resistant recombinant lipase utilizes a culture medium without any products of animal origin or non-characterized mixtures such as tryptone, peptone or lactoserum. A recombinant strain of Yarrowia lipolytica capable of producing an excessive amount of the lipase Lip2 is referred to as YL-LIP2-6C and filed with the Collection Nationale de Cultures de Microorganismes (C. N. C. M.) under a number of I-3542 on December 15 2005. The invention further relates to uses of the recombinant strain.
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Description

[0001] This application is a divisional application of the invention patent application with the filing date of June 15, 2006 and the application number of 200680054940.2 (PCT / FR2006 / 001352). technical field

[0002] The present invention relates to a method for the production of lipase by using Yarrowia lipolytica cells producing an acid-resistant recombinant lipase, which method allows the production of lipase which can be used as a medicine; the present invention also relates to a A strain of Yarrowia lipolytica that overproduces an acid-tolerant recombinant lipase and its use. Background technique

[0003] The daily food intake of human beings is mainly composed of lipids, proteins and sugars. Before they are absorbed, all of these components are subjected to enzyme-catalyzed hydrolysis in the digestive tract. Deficiency in any of these enzymes can thus cause digestive disturbances and lead to considerable malnutrition. That is, for example, pancreatic lipase deficienc...

Claims

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