Construction method of recombinant Yarrowia lipolytica for synthesis of xylitol and strain thereof

A technology of Yarrowia lipolytica and yeast strains, which is applied in the field of fermentative synthesis of xylitol, construction method of recombinant Yarrowia lipolytica and its strains, which can solve the problems of increasing synthesis steps and consuming microbial cell resources
CN110878261AActive Publication Date: 2020-03-13SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2020-03-13

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Abstract

The invention discloses a construction method of recombinant Yarrowia lipolytica for synthesis of xylitol and a strain thereof. Yarrowia lipolytica is used as a synthetic chassis. Through the improvement means of metabolic engineering, the yeast undergoes gene splicing, genes for synthesizing xylitol from glucose, fructose, glycerol and starch as carbon sources are introduced, and metabolic pathways of synthesis by-products are blocked, so that recombinant Yarrowia lipolytica can synthesize xylitol from glucose, fructose, glycerol and starch as carbon sources by fermentation, and an engineering strain for synthesizing xylitol from carbon sources such as glucose and the like is obtained. After fermentation, xylitol crystals are obtained by separation of a bacterial liquid, ion exchange of aclarified fermentation liquid, decolorization, concentration, crystallization and other processes. The construction method of the engineering yeast for synthesizing xylitol from carbon sources such as glucose and the Yarrowia lipolytica strain obtained by the method and for synthesizing xylitol from carbon sources such as glucose can simplify existing methods for chemically synthesizing xylitol and have better application value.
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Description

technical field

[0001] The invention belongs to the field of food biotechnology, and relates to a method for constructing recombinant Yarrowia lipolytica for synthesizing xylitol and strains thereof; more specifically, it relates to using Yarrowia lipolytica as a chassis microorganism through metabolic engineering, genetic engineering and Synthetic biology means to construct a method for fermentatively synthesizing xylitol, using this method to obtain a recombinant Yarrowia lipolytica that can ferment and synthesize xylitol from glucose and other carbon sources, and using the recombinant strain to ferment and synthesize xylitol Methods. Background technique

[0002] Xylitol (xylitol) is a pentahydric alcohol with a CAS number of 87-99-0 and a molecular weight of 152.15 Daltons. It is a common food additive and is often used in the preparation of chewing gum, dairy products, candies and other foods. Reducing the use of sucrose has good effects on preventing oral diseases, re...

Claims

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