Preparation method and application of fermented Chinese herb biological fodder for ruminant animals
A technology for fermenting Chinese herbal medicine and ruminants, applied in animal feed, animal feed, application, etc., can solve the problem that the type and quantity cannot be precisely controlled, prevented and controlled by the feeding environment and rumen system. There is no report on this aspect, and the active ingredients of Chinese herbal medicine Dissolution and other problems can be achieved to enhance the body's adaptability, reduce the use of drugs, and reduce the cost of feeding
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Embodiment 1
[0071] A method for preparing fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:
[0072] Step (1): Aspergillus oryzae, Bacillus natto, Phaffia rhodozyme, Enterococcus faecium and Pediococcus pentosaceae were inoculated in separate sterilized medium for culture, and separate rice Aspergillus, Bacillus natto, Phaffia rhodozyme, Enterococcus faecium and Pediococcus pentosaceae;
[0073] Both the Aspergillus oryzae and Bacillus natto culture medium are LB medium, the Phaffia rhodozyme medium is PDA medium, and the Enterococcus faecium and Pediococcus pentosacea medium are both MRS medium.
[0074] The culture temperature of said Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceae bacteria is 37°C, and the culture temperature of Phaffia rhodozyme is 32°C;
[0075] The culture time of described Aspergillus oryzae, Bacillus natto, Enterococcus faecium, Pediococcus pentosaceae and Phaffia rhodozyme were a...
Embodiment 2
[0092] A method for preparing fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:
[0093] Step (1): Aspergillus oryzae, Bacillus natto, Phaffia rhodozyme, Enterococcus faecium and Pediococcus pentosaceae were inoculated in separate sterilized medium for culture, and separate rice Aspergillus, Bacillus natto, Phaffia rhodozyme, Enterococcus faecium and Pediococcus pentosaceae;
[0094] Both the Aspergillus oryzae and Bacillus natto culture medium are LB medium, the Phaffia rhodozyme medium is PDA medium, and the Enterococcus faecium and Pediococcus pentosacea medium are both MRS medium.
[0095] The culture temperature of said Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceae bacteria is 37°C, and the culture temperature of Phaffia rhodozyme is 25°C;
[0096] The culture time of described Aspergillus oryzae, Bacillus natto, Enterococcus faecium, Pediococcus pentosaceae and Phaffia rhodozyme were a...
Embodiment 3
[0113] A method for preparing fermented Chinese herbal medicine biological feed for ruminants, comprising the following steps:
[0114] Step (1): Aspergillus oryzae, Bacillus natto, Phaffia rhodozyme, Enterococcus faecium and Pediococcus pentosaceae were inoculated in separate sterilized medium for culture, and separate rice Aspergillus, Bacillus natto, Phaffia rhodozyme, Enterococcus faecium and Pediococcus pentosaceae;
[0115] Both the Aspergillus oryzae and Bacillus natto culture medium are LB medium, the Phaffia rhodozyme medium is PDA medium, and the Enterococcus faecium and Pediococcus pentosacea medium are both MRS medium.
[0116] The culture temperature of said Aspergillus oryzae, Bacillus natto, Enterococcus faecium and Pediococcus pentosaceae bacteria is 37°C, and the culture temperature of Phaffia rhodozyme is 27°C;
[0117] The culture time of described Aspergillus oryzae, Bacillus natto, Enterococcus faecium, Pediococcus pentosaceae and Phaffia rhodozyme were a...
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