Novel guide rna/cas endonuclease systems

a technology of endonuclease and guide, which is applied in the field of plant molecular biology, can solve the problems of low specificity, costly and time-consuming preparation, and achieve the effect of reducing the number of endonuclease residues, and improving the specificity
US20190136248A1Inactive Publication Date: 2019-05-09PIONEER HI BRED INT INC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
PIONEER HI BRED INT INC
Publication Date
2019-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

Compositions and methods are provided for novel guide RNA / Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is the 371 national stage entry of International Application Number PCT / US2016 / 032073, filed on 12 May 2016, which claims the benefit of U.S. Provisional Application No. 62 / 162,377, filed May 15, 2015, U.S. Provisional Application No. 62 / 162,353, filed May 15, 2015 and U.S. Provisional Application No. 62 / 196,535, filed Jul. 24, 2015, each of which is incorporated herein in their entirety by reference.FIELD

[0002] The disclosure relates to the field of plant molecular biology, in particular, to compositions for novel guide RNA / Cas endonuclease systems and compositions and methods for altering the genome of a cell.REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY

[0003] The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named 20160502_BB2539PCT_SequenceListing.txt created on May 2, 2016 and having a size 236 kilobytes and is filed concurrentl...

Claims

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